190 research outputs found
Burnout Assessment Tool (BAT): Validity evidence from Brazil and Portugal
The Burnout Assessment Tool (BAT) is gaining increased attention as a sound and innovative instrument in its conceptualization of burnout. BAT has been adapted by several countries, revealing promising validity evidence. This paper aims to present the psychometric properties of the Brazilian and Portuguese versions of the BAT in both the 23-item and 12-item versions. BAT’s validity evidence based on the internal structure (dimensionality, reliability, and measurement invariance) and validity evidence based on the relations to other variables are the focus of research. A cross-sectional study was conducted with two non-probabilistic convenience samples from two countries (N = 3103) one from Brazil (nBrazil = 2217) and one from Portugal (nPortugal = 886). BAT’s original structure was confirmed, and it achieved measurement invariance across countries. Using both classic test theory and item response theory as frameworks, the BAT presented good validity evidence based on the internal structure. Furthermore, the BAT showed good convergent evidence (i.e., work engagement, co-worker support, role clarity, work overload, and negative change). In conclusion, the psychometric properties of the BAT make this freely available instrument a promising way to measure and compare burnout levels of Portuguese and Brazilian workers.info:eu-repo/semantics/publishedVersio
Interação entre o consumo de frutas, verduras e doces e a variação do gene da apolipoproteína AV: a nutrigenética e os níveis de triglicerídeos
Interação entre o consumo de embutidos e a variação no gene da apolipoproteína AV: a nutrigenética e os níveis de triglicerídeos
Perspectivas e desafios para a disseminação em nível internacional para a produção brasileira
PINTA: a web server for network-based gene prioritization from expression data
PINTA (available at http://www.esat.kuleuven.be/pinta/; this web site is free and open to all users and there is no login requirement) is a web resource for the prioritization of candidate genes based on the differential expression of their neighborhood in a genome-wide protein–protein interaction network. Our strategy is meant for biological and medical researchers aiming at identifying novel disease genes using disease specific expression data. PINTA supports both candidate gene prioritization (starting from a user defined set of candidate genes) as well as genome-wide gene prioritization and is available for five species (human, mouse, rat, worm and yeast). As input data, PINTA only requires disease specific expression data, whereas various platforms (e.g. Affymetrix) are supported. As a result, PINTA computes a gene ranking and presents the results as a table that can easily be browsed and downloaded by the user
Genomewide Analysis of Inherited Variation Associated with Phosphorylation of PI3K/AKT/mTOR Signaling Proteins
While there exists a wealth of information about genetic influences on gene expression, less is known about how inherited variation influences the expression and post-translational modifications of proteins, especially those involved in intracellular signaling. The PI3K/AKT/mTOR signaling pathway contains several such proteins that have been implicated in a number of diseases, including a variety of cancers and some psychiatric disorders. To assess whether the activation of this pathway is influenced by genetic factors, we measured phosphorylated and total levels of three key proteins in the pathway (AKT1, p70S6K, 4E-BP1) by ELISA in 122 lymphoblastoid cell lines from 14 families. Interestingly, the phenotypes with the highest proportion of genetic influence were the ratios of phosphorylated to total protein for two of the pathway members: AKT1 and p70S6K. Genomewide linkage analysis suggested several loci of interest for these phenotypes, including a linkage peak for the AKT1 phenotype that contained the AKT1 gene on chromosome 14. Linkage peaks for the phosphorylated:total protein ratios of AKT1 and p70S6K also overlapped on chromosome 3. We selected and genotyped candidate genes from under the linkage peaks, and several statistically significant associations were found. One polymorphism in HSP90AA1 was associated with the ratio of phosphorylated to total AKT1, and polymorphisms in RAF1 and GRM7 were associated with the ratio of phosphorylated to total p70S6K. These findings, representing the first genomewide search for variants influencing human protein phosphorylation, provide useful information about the PI3K/AKT/mTOR pathway and serve as a valuable proof of concept for studies integrating human genomics and proteomics
A construção de uma metodologia observacional para o estudo de crianças em situação de rua: criando um manual de codificação de atividades cotidianas
TargetMine, an Integrated Data Warehouse for Candidate Gene Prioritisation and Target Discovery
Prioritising candidate genes for further experimental characterisation is a
non-trivial challenge in drug discovery and biomedical research in general. An
integrated approach that combines results from multiple data types is best
suited for optimal target selection. We developed TargetMine, a data warehouse
for efficient target prioritisation. TargetMine utilises the InterMine
framework, with new data models such as protein-DNA interactions integrated in a
novel way. It enables complicated searches that are difficult to perform with
existing tools and it also offers integration of custom annotations and in-house
experimental data. We proposed an objective protocol for target prioritisation
using TargetMine and set up a benchmarking procedure to evaluate its
performance. The results show that the protocol can identify known
disease-associated genes with high precision and coverage. A demonstration
version of TargetMine is available at http://targetmine.nibio.go.jp/
Recuperando leituras críticas sobre a avaliação na pós-graduação: dando continuidade à discussão e ao debate
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