185 research outputs found
Cell Autonomous Expression of Perlecan and Plasticity of Cell Shape in Embryonic Muscle ofCaenorhabditis elegans
AbstractPerlecan, a component of the extracellular matrix (ECM), is essential for myofilament formation and muscle attachment inCaenorhabditis elegans.We show here that perlecan is a product of muscle and that it behaves in a cell autonomous fashion. That is, perlecan expressed in an individual muscle cell does not spread beyond the borders of the ECM underlying that cell. Using a polyclonal antibody that recognizes all isoforms of perlecan, we demonstrate that this protein first appears extracellularly at the comma stage (approx. 350 min) of development. We also show that during morphogenesis muscle cells have a heretofore undescribed plasticity of shape. This ability to regulate cell shape allows cells within a muscle quadrant to compensate for missing cells and to form a functional quadrant. A dramatic example of this morphological flexibility can be observed in animals in which the D blastomere has been removed by laser ablation. Such animals, lacking 20 of the 81 embryonic body wall muscle cells, can survive to become viable adult animals indistinguishable from wildtype animals. This demonstrates that the assembly of an embryo via a stereotypic lineage does not preclude a more general regulation during morphogenesis. It appears that embryos are flexible enough to immediately compensate for drastic alterations in tissue composition, a feature of development that may be of general importance during evolution
Cell Autonomous Expression of Perlecan and Plasticity of Cell Shape in Embryonic Muscle ofCaenorhabditis elegans
AbstractPerlecan, a component of the extracellular matrix (ECM), is essential for myofilament formation and muscle attachment inCaenorhabditis elegans.We show here that perlecan is a product of muscle and that it behaves in a cell autonomous fashion. That is, perlecan expressed in an individual muscle cell does not spread beyond the borders of the ECM underlying that cell. Using a polyclonal antibody that recognizes all isoforms of perlecan, we demonstrate that this protein first appears extracellularly at the comma stage (approx. 350 min) of development. We also show that during morphogenesis muscle cells have a heretofore undescribed plasticity of shape. This ability to regulate cell shape allows cells within a muscle quadrant to compensate for missing cells and to form a functional quadrant. A dramatic example of this morphological flexibility can be observed in animals in which the D blastomere has been removed by laser ablation. Such animals, lacking 20 of the 81 embryonic body wall muscle cells, can survive to become viable adult animals indistinguishable from wildtype animals. This demonstrates that the assembly of an embryo via a stereotypic lineage does not preclude a more general regulation during morphogenesis. It appears that embryos are flexible enough to immediately compensate for drastic alterations in tissue composition, a feature of development that may be of general importance during evolution
Interferometry of Aurigae: Characterization of the asymmetric eclipsing disk
We report on a total of 106 nights of optical interferometric observations of
the Aurigae system taken during the last 14 years by four beam
combiners at three different interferometric facilities. This long sequence of
data provides an ideal assessment of the system prior to, during, and after the
recent 2009-2011 eclipse. We have reconstructed model-independent images from
the 10 in-eclipse epochs which show that a disk-like object is indeed
responsible for the eclipse. Using new 3D, time-dependent modeling software, we
derive the properties of the F-star (diameter, limb darkening), determine
previously unknown orbital elements (, ), and access the global
structures of the optically thick portion of the eclipsing disk using both
geometric models and approximations of astrophysically relevant density
distributions. These models may be useful in future hydrodynamical modeling of
the system. Lastly, we address several outstanding research questions including
mid-eclipse brightening, possible shrinking of the F-type primary, and any
warps or sub-features within the disk.Comment: 105 pages, 57 figures. This is an author-created, un-copyedited
version of an article accepted for publication in Astrophysical Journal
Supplement Series. IOP Publishing Ltd is not responsible for any errors or
omissions in this version of the manuscript or any version derived from i
All Six Planets Known to Orbit Kepler-11 Have Low Densities
The Kepler-11 planetary system contains six transiting planets ranging in
size from 1.8 to 4.2 times the radius of Earth. Five of these planets orbit in
a tightly-packed configuration with periods between 10 and 47 days. We perform
a dynamical analysis of the system based upon transit timing variations
observed in more than three years of \ik photometric data. Stellar parameters
are derived using a combination of spectral classification and constraints on
the star's density derived from transit profiles together with planetary
eccentricity vectors provided by our dynamical study. Combining masses of the
planets relative to the star from our dynamical study and radii of the planets
relative to the star from transit depths together with deduced stellar
properties yields measurements of the radii of all six planets, masses of the
five inner planets, and an upper bound to the mass of the outermost planet,
whose orbital period is 118 days. We find mass-radius combinations for all six
planets that imply that substantial fractions of their volumes are occupied by
constituents that are less dense than rock. The Kepler-11 system contains the
lowest mass exoplanets for which both mass and radius have been measured.Comment: 39 pages, 10 figure
Discovery of a Third Transiting Planet in the Kepler-47 Circumbinary System
Of the nine confirmed transiting circumbinary planet systems, only Kepler-47 is known to contain more than one planet. Kepler-47 b (the "inner planet") has an orbital period of 49.5 days and a radius of about 3 Râ. Kepler-47 c (the "outer planet") has an orbital period of 303.2 days and a radius of about 4.7 Râ. Here we report the discovery of a third planet, Kepler-47 d (the "middle planet"), which has an orbital period of 187.4 days and a radius of about 7 Râ. The presence of the middle planet allows us to place much better constraints on the masses of all three planets, where the 1Ïranges are less than 26 Mâ, between 7â43 Mâ, and between 2â5 Mâ for the inner, middle, and outer planets, respectively. The middle and outer planets have low bulk densities, with Ï_(middle) < 0.68 g cm^(â3) and Ï_(outer) < 0.26 g cm^(â3) at the 1Ï level. The two outer planets are "tightly packed," assuming the nominal masses, meaning no other planet could stably orbit between them. All of the orbits have low eccentricities and are nearly coplanar, disfavoring violent scattering scenarios and suggesting gentle migration in the protoplanetary disk
Slice-level diffusion encoding for motion and distortion correction
Advances in microstructural modelling are leading to growing requirements on diffusion MRI acquisitions, namely sensitivity to smaller structures and better resolution of the geometric orientations. The resulting acquisitions contain highly attenuated images that present particular challenges when there is motion and geometric distortion. This study proposes to address these challenges by breaking with the conventional one-volume-one-encoding paradigm employed in conventional diffusion imaging using single-shot Echo Planar Imaging. By enabling free choice of the diffusion encoding on the slice level, a higher temporal sampling of slices with low b-value can be achieved. These allow more robust motion correction, and in combination with a second reversed phase-encoded echo, also dynamic distortion correction. These proposed advances are validated on phantom and adult experiments and employed in a study of eight foetal subjects. Equivalence in obtained diffusion quantities with the conventional method is demonstrated as well as benefits in distortion and motion correction. The resulting capability can be combined with any acquisition parameters including multiband imaging and allows application to diffusion MRI studies in general
A data-driven approach to optimising the encoding for multi-shell diffusion MRI with application to neonatal imaging
Diffusion MRI has the potential to provide important information about the connectivity and microstructure of the human brain during normal and abnormal development, non-invasively and in vivo. Recent developments in MRI hardware and reconstruction methods now permit the acquisition of large amounts of data within relatively short scan times. This makes it possible to acquire more informative multi-shell data, with diffusion-sensitisation applied along many directions over multiple b-value shells. Such schemes are characterised by the number of shells acquired, and the specific b-value and number of directions sampled for each shell. However, there is currently no clear consensus as to how to optimise these parameters. In this work, we propose a means of optimising multi-shell acquisition schemes by estimating the information content of the diffusion MRI signal, and optimising the acquisition parameters for sensitivity to the observed effects, in a manner agnostic to any particular diffusion analysis method that might subsequently be applied to the data. This method was used to design the acquisition scheme for the neonatal diffusion MRI sequence used in the developing Human Connectome Project, which aims to acquire high quality data and make it freely available to the research community. The final protocol selected by the algorithm, and currently in use within the dHCP, consists of 20 b = 0 images and DW images at b = 400, 1000, 2600 s/mm2 with 64, 88, and 128 directions per shell respectively
Pan-cancer Alterations of the MYC Oncogene and Its Proximal Network across the Cancer Genome Atlas
Although theMYConcogene has been implicated incancer, a systematic assessment of alterations ofMYC, related transcription factors, and co-regulatoryproteins, forming the proximal MYC network (PMN),across human cancers is lacking. Using computa-tional approaches, we define genomic and proteo-mic features associated with MYC and the PMNacross the 33 cancers of The Cancer Genome Atlas.Pan-cancer, 28% of all samples had at least one ofthe MYC paralogs amplified. In contrast, the MYCantagonists MGA and MNT were the most frequentlymutated or deleted members, proposing a roleas tumor suppressors.MYCalterations were mutu-ally exclusive withPIK3CA,PTEN,APC,orBRAFalterations, suggesting that MYC is a distinct onco-genic driver. Expression analysis revealed MYC-associated pathways in tumor subtypes, such asimmune response and growth factor signaling; chro-matin, translation, and DNA replication/repair wereconserved pan-cancer. This analysis reveals insightsinto MYC biology and is a reference for biomarkersand therapeutics for cancers with alterations ofMYC or the PMN
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