Episodic synchronization in dynamically driven neurons

We examine the response of type II excitable neurons to trains of synaptic pulses, as a function of the pulse frequency and amplitude. We show that the resonant behavior characteristic of type II excitability, already described for harmonic inputs, is also present for pulsed inputs. With this in mind, we study the response of neurons to pulsed input trains whose frequency varies continuously in time, and observe that the receiving neuron synchronizes episodically to the input pulses, whenever the pulse frequency lies within the neuron's locking range. We propose this behavior as a mechanism of rate-code detection in neuronal populations. The results are obtained both in numerical simulations of the Morris-Lecar model and in an electronic implementation of the FitzHugh-Nagumo system, evidencing the robustness of the phenomenon.Comment: 7 pages, 8 figure

Influence of soil moisture, nitrogen fertilization, and temperature on quality and amino acid composition of Thatcher wheat, The

Includes bibliographical references (pages 227-228).The protein content of Thatcher wheat grown in the growth chamber was increased by reduced water supply, nitrogen fertilization, and higher air temperatures. Soil moisture conditions had a greater influence on protein content at higher temperatures, while the largest responses to nitrogen fertilization were obtained at the medium moisture level. Changes in sedimentation value and mixing time were associated with protein content except for plants grown at 62°F. Apparently the low temperature had an adverse effect on gluten quality. The relative distribution of 9 amino acids was significantly correlated with changes in grain protein content. Only 6 amino acids gave similar high correlations with flour protein content and sedimentation value. These differences are explained on the basis of changes in the morphology of the wheat grain and the proportion of flour proteins

Adverse and Positive Childhood Experiences of Clinical Mental Health Counselors as Predictors of Compassion Satisfaction, Burnout, and Secondary Traumatic Stress

Despite an emphasis on self-care to avoid burnout and increase compassion satisfaction within the counseling profession, there is a dearth of research on the developmental experiences of counselors that may increase the likelihood of burnout. We examined the impact of mental health counselors’ (N = 140) experiences of adverse childhood experiences and positive childhood experiences on their present rates of compassion satisfaction, burnout, and secondary traumatic stress. We used a cross-sectional, non-experimental correlational design and reported descriptive statistics as well as results of multiple regression models. Results indicated significant relationships among counselors’ rates of adverse childhood experiences, positive childhood experiences, and compassion satisfaction and burnout. We include implications for the use of both the adverse and positive childhood experiences assessments in the training of counseling students and supervisees

Absolute Determination of the 22Na(p,g) Reaction Rate in Novae

Gamma-ray telescopes in orbit around the Earth are searching for evidence of the elusive radionuclide 22Na produced in novae. Previously published uncertainties in the dominant destructive reaction, 22Na(p,g)23Mg, indicated new measurements in the proton energy range of 150 to 300 keV were needed to constrain predictions. We have measured the resonance strengths, energies, and branches directly and absolutely by using protons from the University of Washington accelerator with a specially designed beamline, which included beam rastering and cold vacuum protection of the 22Na implanted targets. The targets, fabricated at TRIUMF-ISAC, displayed minimal degradation over a ~ 20 C bombardment as a result of protective layers. We avoided the need to know the stopping power, and hence the target composition, by extracting resonance strengths from excitation functions integrated over proton energy. Our measurements revealed that resonance strengths for E_p = 213, 288, 454, and 610 keV are stronger by factors of 2.4 to 3.2 than previously reported. Upper limits have been placed on proposed resonances at 198-, 209-, and 232-keV. We have re-evaluated the 22Na(p,g) reaction rate, and our measurements indicate the resonance at 213 keV makes the most significant contribution to 22Na destruction in novae. Hydrodynamic simulations including our rate indicate that the expected abundance of 22Na ejecta from a classical nova is reduced by factors between 1.5 and 2, depending on the mass of the white-dwarf star hosting the nova explosion.Comment: 20 pages, 18 figures; shortened paper, accepted in Phys. Rev.

A Role for Topographic Cues in the Organization of Collagenous Matrix by Corneal Fibroblasts and Stem Cells

Human corneal fibroblasts (HCF) and corneal stromal stem cells (CSSC) each secrete and organize a thick stroma-like extracellular matrix in response to different substrata, but neither cell type organizes matrix on tissue-culture polystyrene. This study compared cell differentiation and extracellular matrix secreted by these two cell types when they were cultured on identical substrata, polycarbonate Transwell filters. After 4 weeks in culture, both cell types upregulated expression of genes marking differentiated keratocytes (KERA, CHST6, AQP1, B3GNT7). Absolute expression levels of these genes and secretion of keratan sulfate proteoglycans were significantly greater in CSSC than HCF. Both cultures produced extensive extracellular matrix of aligned collagen fibrils types I and V, exhibiting cornea-like lamellar structure. Unlike HCF, CSSC produced little matrix in the presence of serum. Construct thickness and collagen organization was enhanced by TGF-ß3. Scanning electron microscopic examination of the polycarbonate membrane revealed shallow parallel grooves with spacing of 200–300 nm, similar to the topography of aligned nanofiber substratum which we previously showed to induce matrix organization by CSSC. These results demonstrate that both corneal fibroblasts and stromal stem cells respond to a specific pattern of topographical cues by secreting highly organized extracellular matrix typical of corneal stroma. The data also suggest that the potential for matrix secretion and organization may not be directly related to the expression of molecular markers used to identify differentiated keratocytes