Milieu-adopted in vitro and in vivo differentiation of mesenchymal tissues derived from different adult human CD34-negative progenitor cell clones

Adult mesenchymal stem cells with multilineage differentiation potentially exist in the bone marrow, but have also been isolated from the peripheral blood. The differentiation of stem cells after leaving their niches depends predominately on the local milieu and its new microenvironment, and is facilitated by soluble factors but also by the close cell-cell interaction in a three-dimensional tissue or organ system. We have isolated CD34-negative, mesenchymal stem cell lines from human bone marrow and peripheral blood and generated monoclonal cell populations after immortalization with the SV40 large T-antigen. The cultivation of those adult stem cell clones in an especially designed in vitro environment, including self-constructed glass capillaries with defined growth conditions, leads to the spontaneous establishment of pleomorphic three-dimensional cell aggregates ( spheroids) from the monoclonal cell population, which consist of cells with an osteoblast phenotype and areas of mineralization along with well-vascularized tissue areas. Modifications of the culture conditions favored areas of bone-like calcifications. After the transplantation of the at least partly mineralized human spheroids into different murine soft tissue sites but also a dorsal skinfold chamber, no further bone formation could be observed, but angiogenesis and neovessel formation prevailed instead, enabling the transplanted cells and cell aggregates to survive. This study provides evidence that even monoclonal adult human CD34-negative stem cells from the bone marrow as well as peripheral blood can potentially differentiate into different mesenchymal tissues depending on the local milieu and responding to the needs within the microenvironment. Copyright (C) 2005 S. Karger AG, Basel

Induced Abortion and Life Satisfaction in Germany: The Role of Selection Processes and Short-Term Effects

Unintended pregnancies resulting in induced abortion are occasionally associated with poor psychological well-being. In the literature, this is attributed to either (1) the consequences of abortion, (2) the consequences of unintended pregnancy, or (3) specific selection processes. This longitudinal study addresses these explanations based on data from the German family panel “pairfam” (n = 3,604 women). It compares changes in life satisfaction among different groups of women: Those who had an abortion, those who had a live birth, and those who were not pregnant. A matching procedure ensures the comparability of the groups. The results show that women reported temporarily lower life satisfaction immediately after abortion than similar women following live birth or in absence of pregnancy, while no significant group differences were found in the long run. However, abortion is preceded by significantly lower pre-event life satisfaction than live birth or absence of pregnancy. Persistent poor well-being should therefore primarily be considered a selection criterion for abortions resulting from unintended pregnancies rather than as their consequence

Differential updating and morality: Is the way offenders learn from police detection associated with their personal morals?

The majority of differential deterrability research has investigated whether people differ in the extent to which a perceived threat of sanctions deters them from committing a crime. Less is known about the differential influence of criminal justice intervention on sanction threat perceptions. According to deterrence theory, however, for justice intervention to successfully deter crime, a process of perceptual updating is required. In the current study, we used panel data from German adolescents to supplement the research on differential updating. We applied fixed effects regressions to analyze whether people with weaker or stronger morals update their perceptions of detection risk differently following experiences of police detection. Our findings suggest that they do: risk perceptions increased more in adolescents with weak morals than in adolescents with strong morals when they experienced a higher certainty of detection (a higher detection rate). Combined with previous findings on differential deterrence (by personal morality), our results indicate that deterrence processes may—for individuals with weak morals—play a more critical role in the prevention of crime than previous nondifferential research has suggested

Concurrent TNFRSF1A R92Q and pyrin E230K mutations in a child with multiple sclerosis

We report a 16-year-old female patient with a severe course of multiple sclerosis and concomitant symptoms suggestive of a hereditary autoinflammatory disease. Genetic analyses revealed that she inherited a TNFRSF1A R92Q mutation from her mother and a pyrin E230K mutation from her father. To our knowledge, this is the first report of a patient with severe childhood multiple sclerosis and mutations in two genes which predispose to hereditary autoinflammatory disorders. We speculate that these mutations contribute to early multiple sclerosis manifestation and enhance the inflammatory damage inflicted by the autoimmune response

The impact of Saharan dust and black carbon on albedo and long-term mass balance of an Alpine glacier

Light-absorbing impurities in snow and ice control glacier melt as shortwave radiation represents the main component of the surface energy balance. Here, we investigate the long-term effect of snow impurities, i.e., mineral dust and black carbon (BC), on albedo and glacier mass balance. The analysis was performed over the period 1914–2014 for two sites on Claridenfirn, Swiss Alps, where an outstanding 100-year record of seasonal mass balance measurements is available. Information on atmospheric deposition of mineral dust and BC over the last century was retrieved from two firn/ice cores of high-alpine sites. A combined mass balance and snow/firn layer model was employed to assess the effects of melt and accumulation processes on the impurity concentration at the surface and thus on albedo and glacier mass balance. Compared to pure snow conditions, the presence of Saharan dust and BC lowered the mean annual albedo by 0.04–0.06 depending on the location on the glacier. Consequently, annual melt was increased by 15–19 %, and the mean annual mass balance was reduced by about 280–490 mm w.e. BC clearly dominated absorption which is about 3 times higher than that of mineral dust. The upper site has experienced mainly positive mass balances and impurity layers were continuously buried whereas at the lower site, surface albedo was more strongly influenced by re-exposure of dust and BC-enriched layers due to frequent years with negative mass balances

Incorporating Distributed Debris Thickness in a Glacio-Hydrological Model: Khumbu Himalaya, Nepal

Understanding the future evolution of Himalayan glaciers is important in terms of runoff that provides an essential water source to local populations and has far-reaching downstream impacts. However, the climatic response of glaciers in High-Mountain Asia is complicated by ice stagnation and considerable supraglacial debris coverage, which insulates the ice from warming. Typical runoff modelling only crudely incorporates debris cover and there is currently no consensus on how significantly this may impact future glacier and runoff evolution. Here, a glacio-hydrological model is modified to incorporate fully distributed debris cover, using melt reduction factors that vary depending on debris thickness, and to redistribute mass losses according to observed surface elevation changes. A range of debris thickness data are implemented, including a remote-sensing survey and a modelled debris surface, to analyse the sensitivity of glacier evolution and runoff to possible future debris-cover changes in a series of experiments in the upper Khumbu catchment, Nepal. Simulations are undertaken using climate input data from Regional Climate Model simulations from CORDEX (Coordinated Regional Downscaling Experiment) which are further statistically downscaled using data from the Pyramid meteorological station. Results suggest that the accurate calibration of the model to volume change compensates for the inclusion of distributed debris cover but only if the climatic sensitivity of the calibration period (1999–2010) and the nature of the debris-covered surface remain constant during future simulations. Altering the nature of the debris surface has a significant impact on simulated ice volume, with melt rates under debris suppressed by up to 85 %. The sensitivity of runoff ranges from 60 to 140 million m3 yr-1, although there are considerable uncertainties relating to non-glacial snow melt. Moreover, incorporating locally enhanced melt at ice cliffs into the model also impacts upon volume loss and discharge, with a greater proportion of ice cliffs leading to enhanced volume losses compared to a homogeneous debris surface. Finally, using the most representative model configuration, the future evolution of Khumbu Glacier under various climate scenarios shows continued mass losses with a reduction in volume ranging from 60 % to 97 % by 2100. Runoff trends show an initial increase followed by an eventual decrease, with runoff in 2100 predicted to be 8 % lower than current levels

128Xe and 130Xe: Testing He-shell burning in AGB stars

The s-process branching at 128I has been investigated on the basis of new, precise experimental (n,g) cross sections for the s-only isotopes 128Xe and 130Xe. This branching is unique, since it is essentially determined by the temperature- and density-sensitive stellar decay rates of 128I and only marginally affected by the specific stellar neutron flux. For this reason it represents an important test for He-shell burning in AGB stars. The description of the branching by means of the complex stellar scenario reveals a significant sensitivity to the time scales for convection during He shell flashes, thus providing constraints for this phenomenon. The s-process ratio 128Xe/130Xe deduced from stellar models allows for a (9+-3)% p-process contribution to solar 128Xe, in agreement with the Xe-S component found in meteoritic presolar SiC grains.Comment: 24 pages, 9 figures, accepted for publication in Astophysical Journa

Automated Correctness Proof of Algorithm Variants in Elliptic Curve Cryptography

The Elliptic Curve Cryptography (ECC) is widely known as secure and reliable cryptographic scheme. In many situations the original cryptographic algorithm is modified to improve its efficiency in terms like power consumption or memory consumption which were not in the focus of the original algorithm. For all this modification it is crucial that the functionality and correctness of the original algorithm is preserved. In particular, various projective coordinate systems are applied in order to reduce the computational complexity of elliptic curve encryption by avoiding division in finite fields. This work investigates the possibilities of automated proofs on the correctness of different algorithmic variants. We introduce the theorems which are required to prove the correctness of a modified algorithm variant and the lemmas and definitions which are necessary to prove these goals. The correctness proof of the projective coordinate system transformation has practically been performed with the help of the an interactive formal verification system XeriFun

Phase correlation of laser waves with arbitrary frequency spacing

The theoretically predicted correlation of laser phase fluctuations in Lambda-type interaction schemes is experimentally demonstrated. We show, that the mechanism of correlation in a Lambda scheme is restricted to high frequency noise components, whereas in a double-$\Lambda$ scheme, due to the laser phase locking in closed-loop interaction, it extends to all noise frequencies. In this case the correlation is weakly sensitive to coherence losses. Thus the double-Lambda scheme can be used to correlate e.m. fields with carrier frequency differences beyond the GHz regime.Comment: 4 pages, 4 figure

First experimental demonstration of temporal hypertelescope operation with a laboratory prototype

In this paper, we report the first experimental demonstration of a Temporal HyperTelescope (THT). Our breadboard including 8 telescopes is firstly tested in a manual cophasing configuration on a 1D object. The Point Spread Function (PSF) is measured and exhibits a dynamics in the range of 300. A quantitative analysis of the potential biases demonstrates that this limitation is related to the residual phase fluctuation on each interferometric arm. Secondly, an unbalanced binary star is imaged demonstrating the imaging capability of THT. In addition, 2D PSF is recorded even if the telescope array is not optimized for this purpose.Comment: Accepted for publication in MNRAS. 11 pages, 25 figure