3,153 research outputs found
Tumor bed brachytherapy for locally advanced laryngeal cancer: a feasibility assessment of combination with ferromagnetic hyperthermia
Purpose. To assess the feasibility of adding hyperthermia to an original method of organ-preserving brachytherapy treatment for locally advanced head and neck tumors. Methods and materials. The method involves organ-preserving tumor resection and adjunctive high-dose-rate (HDR) brachytherapy delivered via afterloading catheters. These catheters are embedded in a polymeric implant prepared intraoperatively to fill the resection cavity, allowing precise computer planning of dose distribution in the surrounding at-risk tumor bed tissue. Theoretical and experimental analyzes address the feasibility of heating the tumor bed implant by coupling energy from a 100 kHz magnetic field applied externally into ferromagnetic particles, which are uniformly distributed within the implant. The goal is to combine adjuvant hyperthermia (40 °C–45 °C) to at-risk tissue within 5 mm of the resection cavity for thermal enhancement of radiation and chemotherapy response. Results. A five-year relapse free survival rate of 95.8% was obtained for a select group of 48 male patients with T3N0M0 larynx tumors, when combining organ-preserving surgery with HDR brachytherapy from a tumor bed implant. Anticipating the need for additional treatment in patients with more advanced disease, a theoretical analysis demonstrates the ability to heat at-risk tissue up to 10 mm from the surface of an implant filled with magnetically coupled ferromagnetic balls. Using a laboratory induction heating system, it takes just over 2 min to increase the target tissue temperature by 10 °C using a 19% volume fraction of ferromagnetic spheres in a 2 cm diameter silicone implant. Conclusion. The promising clinical results of a 48 patient pilot study demonstrate the feasibility of a new organ sparing treatment for laryngeal cancer. Anticipating the need for additional therapy, theoretical estimations of potential implant heating are confirmed with laboratory experiments, preparing the way for future implementation of a thermobrachytherapy implant approach for organ-sparing treatment of locally advanced laryngeal cancer
Enhancement of Cavity Cooling of a Micromechanical Mirror Using Parametric Interactions
It is shown that an optical parametric amplifier inside a cavity can
considerably improve the cooling of the micromechanical mirror by radiation
pressure. The micromechanical mirror can be cooled from room temperature 300 K
to sub-Kelvin temperatures, which is much lower than what is achievable in the
absence of the parametric amplifier. Further if in case of a precooled mirror
one can reach millikelvin temperatures starting with about 1 K. Our work
demonstrates the fundamental dependence of radiation pressure effects on photon
statistics.Comment: 14 pages, 7 figure
Species Trees from Highly Incongruent Gene Trees in Rice
Several methods have recently been developed to infer multilocus phylogenies by incorporating information from topological incongruence of the individual genes. In this study, we investigate 2 such methods, Bayesian concordance analysis and Bayesian estimation of species trees. Our test data are a collection of genes from cultivated rice (genus Oryza) and the most closely related wild species, generated using a high-throughput sequencing protocol and bioinformatics pipeline. Trees inferred from independent genes display levels of topological incongruence that far exceed that seen in previous data sets analyzed with these species tree methods. We identify differences in phylogenetic results between inference methods that incorporate gene tree incongruence. Finally, we discuss the challenges of scaling these analyses for data sets with thousands of gene trees and extensive levels of missing data
Science Diplomacy Through Cities: Applying NASA Earth Observations at the Urban Scale
NASA's scientific expertise and data products are enhancing cities' environmental monitoring activities by pioneering applications of remote sensing and model-based Earth Observations at the urban scale. The above activities have greatly benefitted from engaging stakeholders and city practitioners from the start. Further, NASA's collaborations with cities have: Advanced NASA science, in testing new products and validating of satellite datasets, while meeting the needs of city governments. Broadened Rio de Janeiro's regional viewpoint and strengthened its relationships with neighboring cities. Scientific collaborations with cities benefit from: Selecting city partners with a high level of technical capacity and willing to make strong investments in joint projects. Sustained communication and face-to-face interactions. Well-defined deliverables, with dedicated resources and personnel. Pairing global datasets and projections with in situ measurements and local knowledgeSensitivity to local working culture and politics
Structured matrices, continued fractions, and root localization of polynomials
We give a detailed account of various connections between several classes of
objects: Hankel, Hurwitz, Toeplitz, Vandermonde and other structured matrices,
Stietjes and Jacobi-type continued fractions, Cauchy indices, moment problems,
total positivity, and root localization of univariate polynomials. Along with a
survey of many classical facts, we provide a number of new results.Comment: 79 pages; new material added to the Introductio
Local Pheromone Release from Dynamic Polarity Sites Underlies Cell-Cell Pairing during Yeast Mating.
Cell pairing is central for many processes, including immune defense, neuronal connection, hyphal fusion, and sexual reproduction. How does a cell orient toward a partner, especially when faced with multiple choices? Fission yeast Schizosaccharomyces pombe P and M cells, which respectively express P and M factor pheromones [1, 2], pair during the mating process induced by nitrogen starvation. Engagement of pheromone receptors Map3 and Mam2 [3, 4] with their cognate pheromone ligands leads to activation of the Gα protein Gpa1 to signal sexual differentiation [3, 5, 6]. Prior to cell pairing, the Cdc42 GTPase, a central regulator of cell polarization, forms dynamic zones of activity at the cell periphery at distinct locations over time [7]. Here we show that Cdc42-GTP polarization sites contain the M factor transporter Mam1, the general secretion machinery, which underlies P factor secretion, and Gpa1, suggesting that these are sub-cellular zones of pheromone secretion and signaling. Zone lifetimes scale with pheromone concentration. Computational simulations of pair formation through a fluctuating zone show that the combination of local pheromone release and sensing, short pheromone decay length, and pheromone-dependent zone stabilization leads to efficient pair formation. Consistently, pairing efficiency is reduced in the absence of the P factor protease. Similarly, zone stabilization at reduced pheromone levels, which occurs in the absence of the predicted GTPase-activating protein for Ras, leads to reduction in pairing efficiency. We propose that efficient cell pairing relies on fluctuating local signal emission and perception, which become locked into place through stimulation
GHRS and ORFEUS-II Observations of the Highly Ionized Interstellar Medium Toward ESO141-055
We present Goddard High Resolution Spectrograph and ORFEUS-II measurements of
Si IV, CIV, N V, and O VI absorption in the interstellar medium of the Galactic
disk and halo toward the nucleus of the Seyfert galaxy ESO141-055. The high
ionization absorption is strong, with line strengths consistent with the
spectral signature expected for hot (log T = 5-6) collisionally ionized gas in
either a ``Galactic fountain'' or an inhomogeneous medium containing a mixture
of conductive interfaces and turbulent mixing layers. The total O VI column
density of log N ~ 15 suggests that the scale height of O VI is large (>3 kpc)
in this direction. Comparison of the high ion column densities with
measurements for other sight lines indicates that the highly ionized gas
distribution is patchy. The amount of O VI perpendicular to the Galactic plane
varies by at least a factor of ~4 among the complete halo sight lines thus far
studied. In addition to the high ion absorption, lines of low ionization
species are also present in the spectra. With the possible exception of Ar I,
which may have a lower than expected abundance resulting from partial
photoionization of gas along the sight line, the absorption strengths are
typical of those expected for the warm, neutral interstellar medium. The sight
line intercepts a cold molecular cloud with log N(H2) ~ 19. The cloud has an
identifiable counterpart in IRAS 100-micron emission maps of this region of the
sky. We detect a Ly-alpha absorber associated with ESO141-055 at z = 0.03492.
This study presents an enticing glimpse into the interstellar and intergalactic
absorption patterns that will be observed at high spectral resolution by the
Far Ultraviolet Spectroscopic Explorer.Comment: 24 pages + 8 figures, uses aaspp4.sty. Accepted for publication in
Ap
Use of Nanofibers to Strengthen Hydrogels of Silica, Other Oxides, and Aerogels
Research has shown that including up to 5 percent w/w carbon nanofibers in a silica backbone of polymer crosslinked aerogels improves its strength, tripling compressive modulus and increasing tensile stress-at-break five-fold with no increase in density or decrease in porosity. In addition, the initial silica hydrogels, which are produced as a first step in manufacturing the aerogels, can be quite fragile and difficult to handle before cross-linking. The addition of the carbon nanofiber also improves the strength of the initial hydrogels before cross-linking, improving the manufacturing process. This can also be extended to other oxide aerogels, such as alumina or aluminosilicates, and other nanofiber types, such as silicon carbide
Large scale correlations in normal and general non-Hermitian matrix ensembles
We compute the large scale (macroscopic) correlations in ensembles of normal
random matrices with an arbitrary measure and in ensembles of general
non-Hermition matrices with a class of non-Gaussian measures. In both cases the
eigenvalues are complex and in the large limit they occupy a domain in the
complex plane. For the case when the support of eigenvalues is a connected
compact domain, we compute two-, three- and four-point connected correlation
functions in the first non-vanishing order in 1/N in a manner that the
algorithm of computing higher correlations becomes clear. The correlation
functions are expressed through the solution of the Dirichlet boundary problem
in the domain complementary to the support of eigenvalues. The two-point
correlation functions are shown to be universal in the sense that they depend
only on the support of eigenvalues and are expressed through the Dirichlet
Green function of its complement.Comment: 16 pages, 1 figure, LaTeX, submitted to J. Phys. A special issue on
random matrices, minor corrections, references adde
Rapid Genomic Characterization of the Genus Vitis
Next-generation sequencing technologies promise to dramatically accelerate the use of genetic information for crop improvement by facilitating the genetic mapping of agriculturally important phenotypes. The first step in optimizing the design of genetic mapping studies involves large-scale polymorphism discovery and a subsequent genome-wide assessment of the population structure and pattern of linkage disequilibrium (LD) in the species of interest. In the present study, we provide such an assessment for the grapevine (genus Vitis), the world's most economically important fruit crop. Reduced representation libraries (RRLs) from 17 grape DNA samples (10 cultivated V. vinifera and 7 wild Vitis species) were sequenced with sequencing-by-synthesis technology. We developed heuristic approaches for SNP calling, identified hundreds of thousands of SNPs and validated a subset of these SNPs on a 9K genotyping array. We demonstrate that the 9K SNP array provides sufficient resolution to distinguish among V. vinifera cultivars, between V. vinifera and wild Vitis species, and even among diverse wild Vitis species. We show that there is substantial sharing of polymorphism between V. vinifera and wild Vitis species and find that genetic relationships among V. vinifera cultivars agree well with their proposed geographic origins using principal components analysis (PCA). Levels of LD in the domesticated grapevine are low even at short ranges, but LD persists above background levels to 3 kb. While genotyping arrays are useful for assessing population structure and the decay of LD across large numbers of samples, we suggest that whole-genome sequencing will become the genotyping method of choice for genome-wide genetic mapping studies in high-diversity plant species. This study demonstrates that we can move quickly towards genome-wide studies of crop species using next-generation sequencing. Our study sets the stage for future work in other high diversity crop species, and provides a significant enhancement to current genetic resources available to the grapevine genetic community
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