The effect of grass genotype and spring management on the nutritive value of mid-summer ryegrass swards

End of project reportThe objective of this project was to investigate the environmental, morphological and management factors that control reproductive initiation and development in Lolium perenne L. (perennial ryegrass) and their influence on mid-season sward quality. These factors were assessed on eight perennial ryegrass cultivars through spaced plant and plot studies. The first part of this project determined the effects of meteorological conditions and latitude on reproductive initiation and ear emergence of cultivars over two consecutive years. It was concluded that the critical day length requirement for reproductive initiation varies between perennial ryegrass cultivars and is independent of latitude and the normal range of conditions. Using this information a strong correlation (r2 = 0.94) was found between the critical day length for ear initiation and the ten year standardised ear emergence dates of the cultivars. This correlation was sufficiently robust to predict the critical initiation date for any perennial ryegrass cultivar on a UK recommended list or on the EU common catalogue by using their heading dates from the UK Plant Breeders Rights trials at Crossnacreevy. Large variation was observed for secondary initiation and re-heading between cultivars of similar and varying maturity, which is a major factor reducing mid-season sward quality. The propensity for initiation of re-heading was strongly influenced by the severity of defoliation (intense to very lax), but there was also evidence to suggest that critical day length post-solstice, may determine the latest date when further reproductive initiation could occur. Differences in plant growth modes were clearly evident as the sward structure, plant morphology and nutritive compositions differed significantly between cultivars during the mid-season. Defoliation management also significantly affected mid-season sward structure, morphology and nutritive composition. While the effect of defoliation height on the sward physical and chemical compositions was inconclusive, an intensive (30 mm) defoliation resulted in plants returning to a vegetative growth mode earlier compared to a lax (60 mm) defoliation treatment. It was observed that defoliation at a critical growth stage can significantly affect subsequent sward structures. Delaying initial spring defoliation resulted in a greater leaf proportion and swards of greater herbage quality in the plot study. This study, therefore, established the need for more detailed evaluation of cultivars by national testing authorities to allow farmers to select cultivars for grazing use that will optimise animal intake and performance

The spingosine-1-phosphate analogue FTY720 impairs mucosal immunity and clearance of the enteric pathogen Citrobacter rodentium

The sphingosine-1-phosphate (S1P) analogue, FTY720, is therapeutically efficacious in multiple sclerosis and in the prevention of transplant rejection. It prevents migration of lymphocytes to sites of pathology by trapping them within the peripheral lymph nodes, the mesenteric lymph nodes (MLNs) and Peyer's patches. However, evidence suggests that its clinical use may increase the risk of mucosal infections. We investigated the impact of FTY720 treatment on susceptibility to gastrointestinal infection with the mouse enteric pathogen, Citrobacter rodentium (C. rodentium). This attaching and effacing bacterium induces a transient bacterial colitis in immunocompetent mice, which resembles human infection with pathogenic Escherichia coli. FTY720 treatment induced peripheral blood lymphopenia, trapped lymphocytes in the MLNs and prevented clearance of bacteria when mice were infected with luciferase-tagged C. rodentium. FTY720-treated C. rodentium-infected mice had enhanced colonic inflammation, with significantly higher colon mass, colonhistopathology and neutrophil infiltration, when compared with vehicle-infected animals. In addition, FTY720-treated infected mice had significantly lower numbers of colonic dendritic cells, macrophages and T cells. Gene expression analysis demonstrated that FTY720-treated infected mice had an impaired innate immuneresponse and a blunted mucosal adaptive immune response including Th1 cytokines. The data demonstrate that the S1P analogue, FTY720, adversely affects the immune response and clearance of C. rodentium

Physiotherapy for sleep disturbance in chronic low back pain: a feasibility randomised controlled trial

<p>Abstract</p> <p>Background</p> <p>Sleep disturbance is becoming increasingly recognised as a clinically important symptom in people with chronic low back pain (CLBP, low back pain >12 weeks), associated with physical inactivity and depression. Current research and international clinical guidelines recommend people with CLBP assume a physically active role in their recovery to prevent chronicity, but the high prevalence of sleep disturbance in this population may be unknowingly limiting their ability to participate in exercise-based rehabilitation programmes and contributing to poor outcomes. There is currently no knowledge concerning the effectiveness of physiotherapy on sleep disturbance in people with chronic low back pain and no evidence of the feasibility of conducting randomized controlled trials that comprehensively evaluate sleep as an outcome measure in this population.</p> <p>Methods/Design</p> <p>This study will evaluate the feasibility of a randomised controlled trial (RCT), exploring the effects of three forms of physiotherapy (supervised general exercise programme, individualized walking programme and usual physiotherapy, which will serve as the control group) on sleep quality in people with chronic low back pain. A presenting sample of 60 consenting patients will be recruited in the physiotherapy department of Beaumont Hospital, Dublin, Ireland, and randomly allocated to one of the three groups in a concealed manner. The main outcomes will be sleep quality (self-report and objective measurement), and self-reported functional disability, pain, quality of life, fear avoidance, anxiety and depression, physical activity, and patient satisfaction. Outcome will be evaluated at baseline, 3 months and 6 months. Qualitative telephone interviews will be embedded in the research design to obtain feedback from a sample of participants' about their experiences of sleep monitoring, trial participation and interventions, and to inform the design of a fully powered future RCT. Planned analysis will explore trends in the data, effect sizes and clinically important effects (quantitative data), and thematic analysis (qualitative data).</p> <p>Discussion</p> <p>This study will evaluate the feasibility of a randomised controlled trial exploring the effects of three forms of physiotherapy (supervised general exercise programme, individualized walking programme and usual physiotherapy, which will serve as the control group) on sleep quality in people with chronic low back pain.</p> <p>Trial Registration</p> <p>Current controlled trial ISRCTN54009836</p

Objective and subjective assessment of sleep in chronic low back pain patients compared with healthy age and gender matched controls: a pilot study

<p>Abstract</p> <p>Background</p> <p>While approximately 70% of chronic low back pain (CLBP) sufferers complain of sleep disturbance, current literature is based on self report measures which can be prone to bias and no objective data of sleep quality, based exclusively on CLBP are available. In accordance with the recommendations of The American Sleep Academy, when measuring sleep, both subjective and objective assessments should be considered as the two are only modestly correlated, suggesting that each modality assesses different aspects of an individual's sleep experience. Therefore, the purpose of this study was to expand previous research into sleep disturbance in CLBP by comparing objective and subjective sleep quality in participants with CLBP and healthy age and gender matched controls, to identify correlates of poor sleep and to test logistics and gather information prior to a larger study.</p> <p>Methods</p> <p>15 CLBP participants (mean age = 43.8 years (SD = 11.5), 53% female) and 15 healthy controls (mean age = 41.5 years (SD = 10.6), 53% female) consented. All participants completed the Pittsburgh Sleep Quality Index, Insomnia Severity Index, Pittsburgh Sleep Diary and the SF36v2. CLBP participants also completed the Oswestry Disability Index. Sleep patterns were assessed over three consecutive nights using actigraphy. Total sleep time (TST), sleep efficiency (SE), sleep latency onset (SL) and number of awakenings after sleep onset (WASO) were derived. Statistical analysis was conducted using unrelated t-tests and Pearson's product moment correlation co-efficients.</p> <p>Results</p> <p>CLBP participants demonstrated significantly poorer overall sleep both objectively and subjectively. They demonstrated lower actigraphic SE (p = .002) and increased WASO (p = .027) but no significant differences were found in TST (p = .43) or SL (p = .97). Subjectively, they reported increased insomnia (p =< .001), lower SE (p =< .001) and increased SL (p =< .001) but no difference between TST (p = .827) and WASO (p = .055). Statistically significant associations were found between low back pain (p = .021, r = -.589), physical health (p = .003, r = -.713), disability levels (p = .025, r = .576), and subjective sleep quality in the CLBP participants but not with actigraphy.</p> <p>Conclusion</p> <p>CLBP participants demonstrated poorer overall sleep, increased insomnia symptoms and less efficient sleep. Further investigation using a larger sample size and a longer period of sleep monitoring is ongoing.</p

A walking programme and a supervised exercise class versus usual physiotherapy for chronic low back pain: a single-blinded randomised controlled trial. (The Supervised Walking In comparison to Fitness Training for Back Pain (SWIFT) Trial)

BACKGROUND: Chronic low back pain (CLBP) is a persistent disabling condition with rising significant healthcare, social and economic costs. Current research supports the use of exercise-based treatment approaches that encourage people with CLBP to assume a physically active role in their recovery. While international clinical guidelines and systematic reviews for CLBP support supervised group exercise as an attractive first-line option for treating large numbers of CLBP patients at low cost, barriers to their delivery include space and time restrictions in healthcare settings and poor patient attendance. The European Clinical Guidelines have identified the need for research in the use of brief/minimal contact self-activation interventions that encourage participation in physical activity for CLBP. Walking may be an ideally suited form of individualized exercise prescription as it is easy to do, requires no special skills or facilities, and is achievable by virtually all ages with little risk of injury, but its effectiveness for LBP is unproven. METHODS AND DESIGN: This study will be an assessor-blinded randomized controlled trial that will investigate the difference in clinical effectiveness and costs of an individualized walking programme and a supervised general exercise programme compared to usual physiotherapy, which will act as the control group, in people with chronic low back pain. A sample of 246 patients will be recruited in Dublin, Ireland through acute general hospital outpatient physiotherapy departments that provide treatment for people with CLBP. Patients will be randomly allocated to one of the three groups in a concealed manner. The main outcomes will be functional disability, pain, quality of life, fear avoidance, back beliefs, physical activity, satisfaction and costs, which will be evaluated at baseline, and 3, 6 and 12 months [follow-up by pre-paid postage]. Qualitative telephone interviews and focus groups will be embedded in the research design to obtain feedback about participants' experiences of the interventions and trial participation, and to inform interpretation of the quantitative data. Planned analysis will be by intention to treat (quantitative data) and thematic analysis (qualitative data) DISCUSSION: The trial will evaluate the effectiveness of a walking programme and a supervised general exercise programme compared to usual physiotherapy in people with CLBP. TRIAL REGISTRATION: Current controlled trial ISRCTN1759209

The effect of grass genotype and spring management on the nutritive value of mid-summer ryegrass swards

End of project reportThe objective of this project was to investigate the environmental, morphological and management factors that control reproductive initiation and development in Lolium perenne L. (perennial ryegrass) and their influence on mid-season sward quality. These factors were assessed on eight perennial ryegrass cultivars through spaced plant and plot studies. The first part of this project determined the effects of meteorological conditions and latitude on reproductive initiation and ear emergence of cultivars over two consecutive years. It was concluded that the critical day length requirement for reproductive initiation varies between perennial ryegrass cultivars and is independent of latitude and the normal range of conditions. Using this information a strong correlation (r2 = 0.94) was found between the critical day length for ear initiation and the ten year standardised ear emergence dates of the cultivars. This correlation was sufficiently robust to predict the critical initiation date for any perennial ryegrass cultivar on a UK recommended list or on the EU common catalogue by using their heading dates from the UK Plant Breeders Rights trials at Crossnacreevy. Large variation was observed for secondary initiation and re-heading between cultivars of similar and varying maturity, which is a major factor reducing mid-season sward quality. The propensity for initiation of re-heading was strongly influenced by the severity of defoliation (intense to very lax), but there was also evidence to suggest that critical day length post-solstice, may determine the latest date when further reproductive initiation could occur. Differences in plant growth modes were clearly evident as the sward structure, plant morphology and nutritive compositions differed significantly between cultivars during the mid-season. Defoliation management also significantly affected mid-season sward structure, morphology and nutritive composition. While the effect of defoliation height on the sward physical and chemical compositions was inconclusive, an intensive (30 mm) defoliation resulted in plants returning to a vegetative growth mode earlier compared to a lax (60 mm) defoliation treatment. It was observed that defoliation at a critical growth stage can significantly affect subsequent sward structures. Delaying initial spring defoliation resulted in a greater leaf proportion and swards of greater herbage quality in the plot study. This study, therefore, established the need for more detailed evaluation of cultivars by national testing authorities to allow farmers to select cultivars for grazing use that will optimise animal intake and performance

Proteomic Characterization of Armillaria mellea Reveals Oxidative Stress Response Mechanisms and Altered Secondary Metabolism Profiles

Armillaria mellea is a major plant pathogen. Yet, the strategies the organism uses to infect susceptible species, degrade lignocellulose and other plant material and protect itself against plant defences and its own glycodegradative arsenal are largely unknown. Here, we use a combination of gel and MS-based proteomics to profile A. mellea under conditions of oxidative stress and changes in growth matrix. 2-DE and LC-MS/MS were used to investigate the response of A. mellea to H2O2 and menadione/FeCl3 exposure, respectively. Several proteins were detected with altered abundance in response to H2O2, but not menadione/FeCl3 (i.e., valosin-containing protein), indicating distinct responses to these different forms of oxidative stress. One protein, cobalamin-independent methionine synthase, demonstrated a common response in both conditions, which may be a marker for a more general stress response mechanism. Further changes to the A. mellea proteome were investigated using MS-based proteomics, which identified changes to putative secondary metabolism (SM) enzymes upon growth in agar compared to liquid cultures. Metabolomic analyses revealed distinct profiles, highlighting the effect of growth matrix on SM production. This establishes robust methods by which to utilize comparative proteomics to characterize this important phytopathogen