3 research outputs found
Moodee: An Intelligent Mobile Companion for Sensing Your Stress from Your Social Media Postings
In this demo, we build a practical mobile application, Moodee,to help detect and release usersâ psychological stress byleveraging usersâ social media data in online social networks,and provide an interactive user interface to present usersâand friendsâ psychological stress states in an visualized andintuitional way.Given usersâ online social media data as input, Moodee intelligentlyand automatically detects usersâ stress states. Moreover,Moodee would recommend users with different linksto help release their stress. The main technology of this demois a novel hybrid model - a factor graph model combinedwith Deep Neural Network, which can leverage social mediacontent and social interaction information for stress detection.We think that Moodee can be helpful to peopleâs mentalhealth, which is a vital problem in modern world
Artemisitene suppresses rheumatoid arthritis progression via modulating METTL3âmediated N6âmethyladenosine modification of ICAM2Â mRNA in fibroblastâlike synoviocytes
Abstract Background Rheumatoid arthritis (RA) is a chronic autoimmune disease. We previously revealed that the natural compound artemisitene (ATT) exhibits excellent broad anticancer activities without toxicity on normal tissues. Nevertheless, the effect of ATT on RA is undiscovered. Herein, we aim to study the effect and potential mechanism of ATT on RA management. Methods A collagenâinduced arthritis (CIA) mouse model was employed to confirm the antiâRA potential of ATT. Cell Counting Kitâ8Â (CCKâ8) and 5âethynylâ2'âdeoxyuridine (EdU) assays, cell cycle and apoptosis analysis, immunofluorescence, migration and invasion assays, quantitative realâtime PCR (RTâqPCR), Western blot, RNAâsequencing (RNAâseq) analysis, plasmid construction and lentivirus infection, and methylated RNA immunoprecipitation and chromatin immunoprecipitation assays, were carried out to confirm the effect and potential mechanism of ATT on RA management. Results ATT relieved CIA in mice. ATT inhibited proliferation and induced apoptosis of RAâfibroblastâlike synoviocytes (FLSs). ATT restrained RAâFLSs migration and invasion via suppressing epithelialâmesenchymal transition. RNAâsequencing analysis and bioinformatics analysis identified intercellular adhesion molecule 2 (ICAM2) as a promoter of RA progression in RAâFLSs. ATT inhibits RA progression by suppressing ICAM2/phosphoinositide 3âkinase (PI3K)/protein kinase B (AKT)/p300 pathway in RAâFLSs. Moreover, ATT inhibited methyltransferaseâlike 3 (METTL3)âmediated N6âmethyladenosine methylation of ICAM2 mRNA in RAâFLSs. Interestingly, p300 directly facilitated METTL3 transcription, which could be restrained by ATT in RAâFLSs. Importantly, METTL3, ICAM2 and p300 expressions in synovium tissues of RA patients were related to clinical characteristics and therapy response. Conclusions We provided strong evidence that ATT has therapeutic potential for RA management by suppressing proliferation, migration and invasion, in addition to inducing apoptosis of RAâFLSs through modulating METTL3/ICAM2/PI3K/AKT/p300 feedback loop, supplying the fundamental basis for the clinical application of ATT in RA therapy. Moreover, METTL3, ICAM2 and p300 might serve as biomarkers for the therapy response of RA patients