62 research outputs found
Purification and characterization of three laccase isozymes from the white rot fungus Trametes sp. HS-03
Three laccase isozymes (LacI, LacII and LacIII) were isolated from the culture supernatant solution of Trametes sp. HS-03. Diethylaminoethyl (DEAE)-sepharose fast flow anion exchange chromatography and Sephadex G-100 size-exclusion chromatography was performed to achieve electrophoretic homogeneity. The molecular masses (64.2, 60.7 and 38.9 kDa), isoelectric points [pIs (7.3, 4.7 and 3.5), and N-terminal amino acid sequences (G-I-G-P-V, A-I-G-P-T and S-I-G-P-V) were found to be different for the three laccase isozymes. LacI and II have similar thermostability, while LacIII showed better thermostability. LacIII also showed optimal activity at 80°C, with a half-life of 125 min at 70°C. The pI-value of LacI and the molecular mass of LacIII differ significantly from previously described fungal laccases.Keywords: Trametes sp. HS-03, laccase isozymes, purification, characterizatio
Ox-LDL Promotes Migration and Adhesion of Bone Marrow-Derived Mesenchymal Stem Cells via Regulation of MCP-1 Expression
Photometric Variability in the CSTAR Field: Results From the 2008 Data Set
The Chinese Small Telescope ARray (CSTAR) is the first telescope facility
built at Dome A, Antarctica. During the 2008 observing season, the installation
provided long-baseline and high-cadence photometric observations in the i-band
for 18,145 targets within 20 deg2 CSTAR field around the South Celestial Pole
for the purpose of monitoring the astronomical observing quality of Dome A and
detecting various types of photometric variability. Using sensitive and robust
detection methods, we discover 274 potential variables from this data set, 83
of which are new discoveries. We characterize most of them, providing the
periods, amplitudes and classes of variability. The catalog of all these
variables is presented along with the discussion of their statistical
properties.Comment: 38 pages, 11 figures, 4 tables; Accepted for publication in ApJ
Gattini 2010: Cutting Edge Science at the Bottom of the World
The high altitude Antarctic sites of Dome A and the South Pole offer intriguing locations for future large scale optical astronomical
Observatories. The Gattini project was created to measure the optical
sky brightness, large area cloud cover and aurora of the winter-time
sky above such high altitude Antarctic sites. The Gattini-DomeA camera
was installed on the PLATO instrument module as part of the Chinese-led
traverse to the highest point on the Antarctic plateau in January 2008.
This single automated wide field camera contains a suite of Bessel
photometric filters (B, V, R) and a long-pass red filter for the
detection and monitoring of OH emission. We have in hand one complete
winter-time dataset (2009) from the camera that was recently returned
in April 2010.
The Gattini-South Pole UV camera is a wide-field optical camera that in
2011 will measure for the first time the UV properties of the
winter-time sky above the South Pole dark sector. This unique dataset
will consist of frequent images taken in both broadband U and B filters
in addition to high resolution (R similar to 5000) long slit
spectroscopy over a narrow bandwidth of the central field. The camera
is a proof of concept for the 2m-class Antarctic Cosmic Web Imager
telescope, a dedicated experiment to directly detect and map the
redshifted lyman alpha fluorescence or Cosmic Web emission we believe
possible due to the unique geographical qualities of the site.
We present the current status of both projects
Photometry of Variable Stars from Dome A, Antarctica
Dome A on the Antarctic plateau is likely one of the best observing sites on
Earth thanks to the excellent atmospheric conditions present at the site during
the long polar winter night. We present high-cadence time-series aperture
photometry of 10,000 stars with i<14.5 mag located in a 23 square-degree region
centered on the south celestial pole. The photometry was obtained with one of
the CSTAR telescopes during 128 days of the 2008 Antarctic winter.
We used this photometric data set to derive site statistics for Dome A and to
search for variable stars. Thanks to the nearly-uninterrupted synoptic
coverage, we find 6 times as many variables as previous surveys with similar
magnitude limits. We detected 157 variable stars, of which 55% are
unclassified, 27% are likely binaries and 17% are likely pulsating stars. The
latter category includes delta Scuti, gamma Doradus and RR Lyrae variables. One
variable may be a transiting exoplanet.Comment: Accepted for publication in the Astronomical Journal. PDF version
with high-resolution figures available at
http://faculty.physics.tamu.edu/lmacri/papers/wang11.pd
Eclipsing Binaries From the CSTAR Project at Dome A, Antarctica
The Chinese Small Telescope ARray (CSTAR) has observed an area around the
Celestial South Pole at Dome A since 2008. About light curves in the i
band were obtained lasting from March to July, 2008. The photometric precision
achieves about 4 mmag at i = 7.5 and 20 mmag at i = 12 within a 30 s exposure
time. These light curves are analyzed using Lomb--Scargle, Phase Dispersion
Minimization, and Box Least Squares methods to search for periodic signals.
False positives may appear as a variable signature caused by contaminating
stars and the observation mode of CSTAR. Therefore the period and position of
each variable candidate are checked to eliminate false positives. Eclipsing
binaries are removed by visual inspection, frequency spectrum analysis and
locally linear embedding technique. We identify 53 eclipsing binaries in the
field of view of CSTAR, containing 24 detached binaries, 8 semi-detached
binaries, 18 contact binaries, and 3 ellipsoidal variables. To derive the
parameters of these binaries, we use the Eclipsing Binaries via Artificial
Intelligence (EBAI) method. The primary and the secondary eclipse timing
variations (ETVs) for semi-detached and contact systems are analyzed.
Correlated primary and secondary ETVs confirmed by false alarm tests may
indicate an unseen perturbing companion. Through ETV analysis, we identify two
triple systems (CSTAR J084612.64-883342.9 and CSTAR J220502.55-895206.7). The
orbital parameters of the third body in CSTAR J220502.55-895206.7 are derived
using a simple dynamical model.Comment: 41 pages, 12 figures; published online in ApJ
The First Release of the CSTAR Point Source Catalog from Dome A, Antarctica
In 2008 January the 24th Chinese expedition team successfully deployed the
Chinese Small Telescope ARray (CSTAR) to DomeA, the highest point on the
Antarctic plateau. CSTAR consists of four 14.5cm optical telescopes, each with
a different filter (g, r, i and open) and has a 4.5degree x 4.5degree field of
view (FOV). It operates robotically as part of the Plateau Observatory, PLATO,
with each telescope taking an image every 30 seconds throughout the year
whenever it is dark. During 2008, CSTAR #1 performed almost flawlessly,
acquiring more than 0.3 million i-band images for a total integration time of
1728 hours during 158 days of observations. For each image taken under good sky
conditions, more than 10,000 sources down to 16 mag could be detected. We
performed aperture photometry on all the sources in the field to create the
catalog described herein. Since CSTAR has a fixed pointing centered on the
South Celestial Pole (Dec =-90 degree), all the sources within the FOV of CSTAR
were monitored continuously for several months. The photometric catalog can be
used for studying any variability in these sources, and for the discovery of
transient sources such as supernovae, gamma-ray bursts and minor planets.Comment: 1 latex file and 9 figures The paper is accepted by PAS
The sky brightness and transparency in i-band at Dome A, Antarctica
The i-band observing conditions at Dome A on the Antarctic plateau have been
investigated using data acquired during 2008 with the Chinese Small Telescope
ARray. The sky brightness, variations in atmospheric transparency, cloud cover,
and the presence of aurorae are obtained from these images. The median sky
brightness of moonless clear nights is 20.5 mag arcsec^{-2} in the SDSS
band at the South Celestial Pole (which includes a contribution of about 0.06
mag from diffuse Galactic light). The median over all Moon phases in the
Antarctic winter is about 19.8 mag arcsec^{-2}. There were no thick clouds in
2008. We model contributions of the Sun and the Moon to the sky background to
obtain the relationship between the sky brightness and transparency. Aurorae
are identified by comparing the observed sky brightness to the sky brightness
expected from this model. About 2% of the images are affected by relatively
strong aurorae.Comment: There are 1 Latex file and 14 figures accepted by A
Airglow and Aurorae at Dome A, Antarctica
Despite the absence of artificial light pollution at Antarctic plateau sites such as Dome A, other factors such as airglow, aurorae, and extended periods of twilight have the potential to adversely affect optical observations. We present a statistical analysis of the airglow and aurorae at Dome A using spectroscopic data from Nigel, an optical/near-IR spectrometer operating in the 300–850 nm range. These data complement photometric images from Gattini, a wide-field (90°) CCD camera with B, V, and R filters, allowing the background sky brightness to be disentangled from the various airglow and auroral emission lines. The median auroral contribution to the B, V, and R photometric bands is found to be 22.9, 23.4, and 23.0 mag arcsec^(-2), respectively. Auroral emissions most frequently occur between 10–23 hr local time, when up to 50% of observations are above airglow-level intensities. While infrequent, the strongest emissions detected occurred in the hours just prior to magnetic midnight. We are also able to quantify the amount of annual dark time available as a function of wavelength, as well as in the standard BVR photometric bands. On average, twilight ends when the Sun reaches a zenith distance of 102.6°
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