368 research outputs found
Ecologically Sensitive Wetlands on Maui: Groundwater Protection Strategy for Hawaii
The EPA Ground-Water Protection Strategy has established differential protection levels based on the beneficial uses of groundwaters. Groundwater resources that are: (1) Irreplaceable sources of drinking water; and/or (2) Ecologically Vital are designated as of unusually high value. To determine those groundwaters that meet the EPA criteria for qualifying as “Ecologically Vital” we have examined 24 groundwater based (wetland) ecological systems on the island of Maui. An inventory of the physical, biological and cultural characteristics of each area including “red flag” features has been developed and coded. Using this “habitat code” a rating system that reflects the sensitivity, i.e. “uniqueness” of “nonrenewable” attributes of each system was designed and 18 “ecologically vital” habitats were identified that meet the EPA criteria for Class 1 level of groundwater protection. Insufficient information was available to determine the sensitivity of one of the sites.Department of Health, State of Hawai
Quantum games of asymmetric information
We investigate quantum games in which the information is asymmetrically
distributed among the players, and find the possibility of the quantum game
outperforming its classical counterpart depends strongly on not only the
entanglement, but also the informational asymmetry. What is more interesting,
when the information distribution is asymmetric, the contradictive impact of
the quantum entanglement on the profits is observed, which is not reported in
quantum games of symmetric information.Comment: 5 pages, 3 figure
(Arg) 9 -SH2 superbinder: A novel promising anticancer therapy to melanoma by blocking phosphotyrosine signaling
Background: Melanoma is a malignant tumor with high misdiagnosis rate and poor prognosis. The bio-targeted therapy is a prevailing method in the treatment of melanoma; however, the accompanying drug resistance is inevitable. SH2 superbinder, a triple-mutant of the Src Homology 2 (SH2) domain, shows potent antitumor ability by replacing natural SH2-containing proteins and blocking multiple pY-based signaling pathways. Polyarginine (Arg) 9 , a powerful vector for intracellular delivery of large molecules, could transport therapeutic agents across cell membrane. The purpose of this study is to construct (Arg) 9 -SH2 superbinder and investigate its effects on melanoma cells, expecting to provide potential new approaches for anti-cancer therapy and overcoming the unavoidable drug resistance of single-targeted antitumor agents. Methods: (Arg) 9 and SH2 superbinder were fused to form (Arg) 9 -SH2 superbinder via genetic engineering. Pull down assay was performed to identify that (Arg) 9 -SH2 superbinder could capture a wide variety of pY proteins. Immunofluorescence was used to detect the efficiency of (Arg) 9 -SH2 superbinder entering cells. The proliferation ability was assessed by MTT and colony formation assay. In addition, wound healing and transwell assay were performed to evaluate migration of B16F10, A375 and A375/DDP cells. Moreover, apoptosis caused by (Arg) 9 -SH2 superbinder was analyzed by flow cytometry-based Annexin V/PI. Furthermore, western blot revealed that (Arg) 9 -SH2 superbinder influenced some pY-related signaling pathways. Finally, B16F10 xenograft model was established to confirm whether (Arg) 9 -SH2 superbinder could restrain the growth of tumor. Results: Our data showed that (Arg) 9 -SH2 superbinder had the ability to enter melanoma cells effectively and displayed strong affinities for various pY proteins. Furthermore, (Arg) 9 -SH2 superbinder could repress proliferation, migration and induce apoptosis of melanoma cells by regulating PI3K/AKT, MAPK/ERK and JAK/STAT signaling pathways. Importantly, (Arg) 9 -SH2 superbinder could significantly inhibit the growth of tumor in mice. Conclusions: (Arg) 9 -SH2 superbinder exhibited high affinities for pY proteins, which showed effective anticancer ability by replacing SH2-containing proteins and blocking diverse pY-based pathways. The remarkable ability of (Arg) 9 -SH2 superbinder to inhibit cancer cell proliferation and tumor growth might open the door to explore the SH2 superbinder as a therapeutic agent for cancer treatment
Manifestation of photonic band structure in small clusters of spherical particles
We study the formation of the photonic band structure in small clusters of
dielectric spheres. The first signs of the band structure, an attribute of an
infinite crystal, can appear for clusters of 5 particles. Density of resonant
states of a cluster of 32 spheres may exhibit a well defined structure similar
to the density of electromagnetic states of the infinite photonic crystal. The
resonant mode structure of finite-size aggregates is shown to be insensitive to
random displacements of particles off the perfect lattice positions as large as
half-radius of the particle. The results were obtained by an efficient
numerical method, which relates the density of resonant states to the the
scattering coefficients of the electromagnetic scattering problem. Generalized
multisphere Mie (GMM) solution was used to obtain scattering matrix elements.
These results are important to miniature photonic crystal design as well as
understanding of light localization in dense random media.Comment: 4 pages, 2 figure
Acute Encephalopathy Associated with Influenza A Infection in Adults
We report acute encephalopathy associated with influenza A infection in 3 adults. We detected high cerebrospinal fluid (CSF) and plasma concentrations of CXCL8/IL-8 and CCL2/MCP-1 (CSF/plasma ratios >3), and interleukin-6, CXCL10/IP-10, but no evidence of viral neuroinvasion. Patients recovered without sequelae. Hyperactivated cytokine response may play a role in pathogenesis
Human Metapneumovirus Detection in Patients with Severe Acute Respiratory Syndrome
We used a combination approach of conventional virus isolation and molecular techniques to detect human metapneumovirus (HMPV) in patients with severe acute respiratory syndrome (SARS). Of the 48 study patients, 25 (52.1%) were infected with HMPV; 6 of these 25 patients were also infected with coronavirus, and another 5 patients (10.4%) were infected with coronavirus alone. Using this combination approach, we found that human laryngeal carcinoma (HEp-2) cells were superior to rhesus monkey kidney (LLC-MK2) cells commonly used in previous studies for isolation of HMPV. These widely available HEp-2 cells should be included in conjunction with a molecular method for cell culture followup to detect HMPV, particularly in patients with SARS
The Planetary Nebula Luminosity Function at the Dawn of Gaia
The [O III] 5007 Planetary Nebula Luminosity Function (PNLF) is an excellent
extragalactic standard candle. In theory, the PNLF method should not work at
all, since the luminosities of the brightest planetary nebulae (PNe) should be
highly sensitive to the age of their host stellar population. Yet the method
appears robust, as it consistently produces < 10% distances to galaxies of all
Hubble types, from the earliest ellipticals to the latest-type spirals and
irregulars. It is therefore uniquely suited for cross-checking the results of
other techniques and finding small offsets between the Population I and
Population II distance ladders. We review the calibration of the method and
show that the zero points provided by Cepheids and the Tip of the Red Giant
Branch are in excellent agreement. We then compare the results of the PNLF with
those from Surface Brightness Fluctuation measurements, and show that, although
both techniques agree in a relative sense, the latter method yields distances
that are ~15% larger than those from the PNLF. We trace this discrepancy back
to the calibration galaxies and argue that, due to a small systematic error
associated with internal reddening, the true distance scale likely falls
between the extremes of the two methods. We also demonstrate how PNLF
measurements in the early-type galaxies that have hosted Type Ia supernovae can
help calibrate the SN Ia maximum magnitude-rate of decline relation. Finally,
we discuss how the results from space missions such as Kepler and Gaia can help
our understanding of the PNLF phenomenon and improve our knowledge of the
physics of local planetary nebulae.Comment: 12 pages, invited review at the conference "The Fundamental Cosmic
Distance Scale: State of the Art and Gaia Perspective", to appear in
Astrophysics and Space Scienc
- …