Spectrographic analysis of bismuth-tin eutectic alloys by spark-ignited low-voltage ac-arc excitation

Spectrographic method determines individual stainless steel components in molten bismuth-42 w/o tin eutectic to determine the solubility of Type 304 stainless steels. It utilizes the high sensitivity and precision of the spark-ignited, low-voltage ac-arc excitation of samples rendered homogeneous by dissolution

An Instrument to Assess Client-Server Architecture in an Organizational Setting

This study reports the final results of a delphi panel procedure used to develop an instrument to assess the client-server system architecture in an organization. At present there is no formal instrument that allows an information systems manager to assess an existing CSS architecture. A delphi panel was used to develop a formal instrument for CSS assessment

Development of an Autonomous, Dual Chamber Bioreactor for the Growth of 3-Dimensional Epithelial-Stromal Tissues in Microgravity

We are developing a novel, autonomous bioreactor that can provide for the growth and maintenance in microgravity of 3D organotypic epithelialstromal cultures that require an airliquid interface. These complex 3D tissue models accurately represent the morphological features, differentiation markers, and growth characteristics observed in normal human epithelial tissues, including the skin, esophagus, lung, breast, pancreas, and colon. However, because of their precise and complex culture requirements, including that of an airliquid interface, these 3D models have yet to be utilized for life sciences research aboard the International Space Station. The development of a bioreactor for these cultures will provide the capability to perform biological research on the ISS using these realistic, tissuelike human epithelialstromal cell models and will contribute significantly to advances in fundamental space biology research on questions regarding microgravity effects on normal tissue development, aging, cancer, and other disease processes. It will also allow for the study of how combined stressors, such as microgravity with radiation and nutritional deficiencies, affect multiple biological processes and will provide a platform for conducting countermeasure investigations on the ISS without the use of animal models. The technology will be autonomous and consist of a cell culture chamber that provides for airliquid, liquidliquid, and liquidair exchanges within the chambers while maintaining the growth and development of the biological samples. The bioreactor will support multiple tissue types and its modular design will provide for incorporation of addon capabilities such as microfluidics drug delivery, media sampling, and in situ biomarker analysis. Preliminary flight testing of the hardware will be conducted on a parabolic platform through NASA's Flight Opportunities Program

Genotoxic Effects of Low- and High-LET Radiation on Human Epithelial Cells Grown in 2-D Versus 3-D Culture

Risk estimation for radiation-induced cancer relies heavily on human epidemiology data obtained from terrestrial irradiation incidents from sources such as medical and occupational exposures as well as from the atomic bomb survivors. No such data exists for exposures to the types and doses of high-LET radiation that will be encountered during space travel; therefore, risk assessment for space radiation requires the use of data derived from cell culture and animal models. The use of experimental models that most accurately replicate the response of human tissues is critical for precision in risk projections. This work compares the genotoxic effects of radiation on normal human epithelial cells grown in standard 2-D monolayer culture compared to 3-D organotypic co-culture conditions. These 3-D organotypic models mimic the morphological features, differentiation markers, and growth characteristics of fully-differentiated normal human tissue and are reproducible using defined components. Cultures were irradiated with 2 Gy low-LET gamma rays or varying doses of high-LET particle radiation and genotoxic damage was measured using a modified cytokinesis block micronucleus assay. Our results revealed a 2-fold increase in residual damage in 2 Gy gamma irradiated cells grown under organotypic culture conditions compared to monolayer culture. Irradiation with high-LET particle radiation gave similar results, while background levels of damage were comparable under both scenarios. These observations may be related to the phenomenon of "multicellular resistance" where cancer cells grown as 3-D spheroids or in vivo exhibit an increased resistance to killing by chemotherapeutic agents compared to the same cells grown in 2-D culture. A variety of factors are likely involved in mediating this process, including increased cell-cell communication, microenvironment influences, and changes in cell cycle kinetics that may promote survival of damaged cells in 3-D culture that would otherwise die or be rendered reproductively inactive in 2-D culture

Reducing Polarization Mode Dispersion With Controlled Polarization Rotations

One of the fundamental limitations to high bit rate, long distance, telecommunication in optical fibers is Polarization Mode Dispersion (PMD). Here we introduce a conceptually new method to reduce PMD in optical fibers by carrying out controlled rotations of polarization at predetermined locations along the fiber. The distance between these controlled polarization rotations must be less than both the beat length and the mode coupling length of the fiber. This method can also be combined with the method in which the fiber is spun while it drawn. The incidence of imperfections on the efficiency of the method is analysed.Comment: 4 page

Flutter analysis of a supersonic cascade in time domain using an ADI Euler solver

The aeroelastic stability of a two-dimensional cascade oscillating in supersonic axial flow is analyzed in the time domain. The aeroelastic model consists of a single degree of freedom typical section structural model for each blade of the cascade and an unsteady two-dimensional cascade aerodynamic model based on the Euler equations. The Euler equations are solved using a time accurate Alternating Direction Implicit (ADI) solution scheme. The aeroelastic equations are integrated in time. The effect of interblade phase angle is included in the aeroelastic analysis by an appropriate choice of initial and boundary conditions. Flutter predictions are obtained from the time response of a flat plate cascade in single degree of freedom pitching motion. The results correlate well with those obtained from a separate frequency domain flutter analysis for all values of interblade phase angles considered. Flutter results are then presented for cascades having airfoil sections representative of a supersonic throughflow fan. The validity of the time integration method for a cascade of airfoils at various interblade phase angles is demonstrated

Simulations of Baryon Oscillations

The coupling of photons and baryons by Thomson scattering in the early universe imprints features in both the Cosmic Microwave Background (CMB) and matter power spectra. The former have been used to constrain a host of cosmological parameters, the latter have the potential to strongly constrain the expansion history of the universe and dark energy. Key to this program is the means to localize the primordial features in observations of galaxy spectra which necessarily involve galaxy bias, non-linear evolution and redshift space distortions. We present calculations, based on mock catalogs produced from high-resolution N-body simulations, which show the range of behaviors we might expect of galaxies in the real universe. We investigate physically motivated fitting forms which include the effects of non-linearity, galaxy bias and redshift space distortions and discuss methods for analysis of upcoming data. In agreement with earlier work, we find that a survey of several Gpc^3 would constrain the sound horizon at z~1 to about 1%.Comment: 33 pages, to appear in Astroparticle Physics. Discussion of Blake & Glazebrook procedure changed, minor edits to match version accepted by the journa

Optimal Controller Identification for multivariable non-minimum phase systems

This paper extends the formulation of a data-driven control method - the Optimal Controller Identification (OCI) - to cope with non-minimum phase (NMP) systems, without a priori knowledge of the NMP transmission zero, i.e. without obtaining a prior model of the plant - as in any data-driven approach. The method is based on the Model Reference paradigm, in which the desired closed-loop performance is specified by means of a closed-loop transfer function - the reference model. Considering a convenient parametrization of the latter and a flexible performance criterion, it is possible to identify the NMP transmission zeros of the plant along with the optimal controller parameters, as it will be shown. Both diagonal and block-triangular reference model structures are treated in detail. Simulation examples show the effectiveness of the proposed approach

Organizational learning and emotion: constructing collective meaning in support of strategic themes

Missing in the organizational learning literature is an integrative framework that reflects the emotional as well as the cognitive dynamics involved. Here, we take a step in this direction by focusing in depth over time (five years) on a selected organization which manufactures electronic equipment for the office industry. Drawing on personal construct theory, we define organizational learning as the collective re-construal of meaning in the direction of strategically significant themes. We suggest that emotions arise as members reflect on progress or lack of progress in achieving organizational learning. Our evidence suggests that invalidation – where organizational learning fails to correspond with expectations – gives rise to anxiety and frustration, while validation – where organizational learning is aligned with or exceeds expectations – evokes comfort or excitement. Our work aims to capture the key emotions involved as organizational learning proceeds