Three-dimensional Migration of Neurites Is Mediated by Adhesion Site Density and Affinity

Three-dimensional neurite outgrowth rates within fibrin matrices that contained variable amounts of RGD peptides were shown to depend on adhesion site density and affinity. Bi-domain peptides with a factor XIIIa substrate in one domain and a RGD sequence in the other domain were covalently incorporated into fibrin gels during coagulation through the action of the transglutaminase factor XIIIa, and the RGD-dependent effect on neurite outgrowth was quantified, employing chick dorsal root ganglia cultured two- and three-dimensionally within the modified fibrin. Two separate bi-domain peptides were synthesized, one with a lower binding affinity linear RGD domain and another with a higher binding affinity cyclic RGD domain. Both peptides were cross-linked into fibrin gels at concentrations up to 8.2 mol of peptide/mol of fibrinogen, and their effect on neurite outgrowth was measured. Both two- and three-dimensional neurite outgrowth demonstrated a bi-phasic dependence on RGD concentration for both the linear and cyclic peptide, with intermediate adhesion site densities yielding maximal neurite extension and higher densities inhibiting outgrowth. The adhesion site density that yielded maximal outgrowth depended strongly on adhesion site affinity in both two and three dimensions, with lower densities of the higher affinity ligand being required (0.8-1.7 mol/mol for the linear peptide versus 0.2 mol/mol for the cyclic peptide yielding maximum neurite outgrowth rates in three-dimensional cultures)

Total Retroflexion of the Iris.

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Promotion of Advance Care Planning for Individuals Experiencing Homelessness

Vulnerable populations in the United States experience disparities in access to advance care planning and may have significant unmet healthcare needs at the end of life, including unrelieved suffering. People who are homeless have increased morbidity and mortality risks, yet lack opportunities to discuss end-of-life preferences. A Doctor of Nursing Practice project consisted of designing, implementing, and evaluating an educational intervention in two settings for nurse practitioners and other clinicians on advance care planning with individuals experiencing homelessness. The intervention was associated with increased knowledge and confidence in advance care planning ability. Participants expressed positive intention to change individual practice based on learnings. Lack of time and patient discomfort with end-of-life topics were identified as barriers to changing practice regarding advance care planning. Educational interventions on advance care planning for clinicians were effective and may help to increase patient access to this important component of care. Advance care planning is a crucial antecedent to receiving patient-centered care at the end of life, particularly for vulnerable populations

INFORMATION QUALITY, TECHNOLOGY DEPRECIATION, AND BT COTTON ADOPTION IN THE SOUTHEAST

In 1996, Bt cotton became one of the first genetically engineered crops to be available commercially. This study focuses on the various sources and quality of information about Bt cotton profitability available to farmers in the Southeast and assesses the relative importance of such information in the farmers' adoption decisions. A model of the individual decision to adopt is developed to incorporate two recent theories of the role of information quality (the "effective information" hypothesis and the "popularity" hypothesis) as well as the effect of current technology depreciation. The data show some support for all three factors as determinants of adoption.Crop Production/Industries,

Biocompatible microcapsules

Biocompatible microcapsules useful for transplanting foreign material into an animal body, and the method of their production, are described, wherein the microcapsules contain an outermost layer of water soluble non-ionic polymers such as PEO to create resistance to cell adhesion on the surface of the microcapsules.Board of Regents, University of Texas Syste

Biocompatible microcapsules

Biocompatible microcapsules useful for transplanting foreign material into an animal body, and the method of their production, are described, wherein the microcapsules contain an outermost layer of water soluble non-ionic polymers such as PEO to create resistance to cell adhesion on the surface of the microcapsules.Board of Regents, University of Texas Syste

A seed-diffusion model for tropical tree diversity patterns

Diversity patterns of tree species in a tropical forest community are approached by a simple lattice model and investigated by Monte Carlo simulations using a backtracking method. Our spatially explicit neutral model is based on a simple statistical physics process, namely the diffusion of seeds. The model has three parameters: the speciation rate, the size of the meta-community in which the studied tree-community is embedded, and the average surviving time of the seeds. By extensive computer simulations we aim the reproduction of relevant statistical measures derived from the experimental data of the Barro Colorado Island tree census in year 1995. The first two parameters of the model are fixed to known values, characteristic of the studied community, thus obtaining a model with only one freely adjustable parameter. As a result of this, the average number of species in the considered territory, the relative species abundance distribution, the species-area relationship and the spatial auto-correlation function of the individuals in abundant species are simultaneously fitted with only one parameter which is the average surviving time of the seeds.Comment: 12 pages, 5 figure

Lunar base CELSS: A bioregenerative approach

During the twenty-first century, human habitation of a self-sustaining lunar base could become a reality. To achieve this goal, the occupants will have to have food, water, and an adequate atmosphere within a carefully designed environment. Advanced technology will be employed to support terrestrial life-sustaining processes on the Moon. One approach to a life support system based on food production, waste management and utilization, and product synthesis is outlined. Inputs include an atmosphere, water, plants, biodegradable substrates, and manufacutured materials such as fiberglass containment vessels from lunar resources. Outputs include purification of air and water, food, and hydrogen (H2) generated from methane (CH4). Important criteria are as follows: (1) minimize resupply from Earth; and (2) recycle as efficiently as possible

The burrowing beetles of the genus Mycotrupes (Coleoptera: Scarabaeidae: Geotrupinae)

http://deepblue.lib.umich.edu/bitstream/2027.42/56329/1/MP084.pd