Effects of radiotransmitters on fecal glucocorticoid metabolite levels of three-toed box turtles in captivity

The increased use of radio-telemetry for studying movement, resource selection, and population demographics in reptiles necessitates closer examination of the assumption that radiotransmitter attachment does not bias study results. We determined the effects of radiotransmitter attachment on fecal glucocorticoid metabolite levels of wild three-toed box turtles (Terrapene carolina triunguis) in captivity. During May 2002 we captured 11 adult three-toed box turtles in central Missouri. We housed turtles in individual pens in a semi-natural outdoor setting. We radio-tagged 6 turtles, and the remaining 5 turtles served as controls. We captured and handled all turtles similarly during treatments. We collected feces daily prior to attachment (14 June–05 July 2002), while transmitters were attached (06 July–02 August 2002), and after transmitters were removed (03 August–24 August 2002). We conducted a standard assay validation and found that the assay accurately and precisely quantified fecal glucocorticoid metabolites of box turtles. We did not find a significant effect of radiotransmitter attachment on fecal glucocorticoid metabolite levels of three-toed box turtles (F1, 9 =0.404, P=0.541). Fecal glucocorticoid metabolite levels of control and treatment turtles increased significantly during the study (F2,166=7.874, P= 0.001), but there was no treatment:period interaction (F2,166 = 0.856, P = 0.427). Additionally, we did not find a significant relationship between glucocorticoid metabolite levels and time in captivity (r2=0.01, F1,179=2.89, P=0.091) or maximum daily temperature (r2\u3c0.01, F1,179=0.301, P=0.584). Our results suggested that radiotransmitter attachment did not significantly increase fecal glucocorticoid metabolite levels in adult three-toed box turtles; however, we conducted our study in captivity and sample sizes were small. Thus, more research is needed to assess potential effects of radiotransmitters on turtles in the wild. We believe this study is the first to validate the use of fecal glucocorticoid metabolite measures for reptiles, which might prove useful in other research studies

Central Executive Dysfunction and Deferred Prefrontal Processing in Veterans with Gulf War Illness.

Gulf War Illness is associated with toxic exposure to cholinergic disruptive chemicals. The cholinergic system has been shown to mediate the central executive of working memory (WM). The current work proposes that impairment of the cholinergic system in Gulf War Illness patients (GWIPs) leads to behavioral and neural deficits of the central executive of WM. A large sample of GWIPs and matched controls (MCs) underwent functional magnetic resonance imaging during a varied-load working memory task. Compared to MCs, GWIPs showed a greater decline in performance as WM-demand increased. Functional imaging suggested that GWIPs evinced separate processing strategies, deferring prefrontal cortex activity from encoding to retrieval for high demand conditions. Greater activity during high-demand encoding predicted greater WM performance. Behavioral data suggest that WM executive strategies are impaired in GWIPs. Functional data further support this hypothesis and suggest that GWIPs utilize less effective strategies during high-demand WM

Assessing the suitability of three proxy sources for the development of detectors of special nuclear materials

Numerous techniques and equipment have been developed to provide a capability for the detection of special nuclear materials (SNM), but due to the necessary security measures surrounding these materials alternate, or proxy, neutron sources are often utilised in their stead. In this paper we report the neutron and gamma pulse shape discrimination response of plastic scintillator to mixed neutron/gamma beams produced from two radionuclide neutron sources, and also from an SNM source of weapons-grade plutonium. We discuss the suitability of using radionuclide sources, with appropriate shielding configurations as proxy sources for SNM.A 3σnth-γ discrimination level has been achieved for an SNM source at a low-level energy threshold of ~220 keVee when a shielding configuration of 5 cm of lead was implemented. Varying amounts of lead and high-density polyethylene (HDPE) shielding were also investigated with the 3σ limit being reached by ~240 keVee.This work shows that an AmBe neutron source serves as an appropriate SNM proxy achieving a comparable value for figure of merit above ~1 MeVee. For energies below 1 MeVee down to ~100 keVee a closer approximation of the expected FoM for SNM can be attained when using 252Cf as a proxy source or by utilising an 'enhanced' AmBe source with the addition of a further low energy γ ray source

Identity of the universal repulsive-core singularity with Yang-Lee edge criticality

Lattice and continuum fluid models with repulsive-core interactions typically display a dominant, critical-type singularity on the real, negative activity axis. Lai and Fisher recently suggested, mainly on numerical grounds, that this repulsive-core singularity is universal and in the same class as the Yang-Lee edge singularities, which arise above criticality at complex activities with positive real part. A general analytic demonstration of this identification is presented here using a field-theory approach with separate representation of the repulsive and attractive parts of the pair interactions.Comment: 6 pages, 3 figure

e-Fungi: a data resource for comparative analysis of fungal genomes.

BACKGROUND: The number of sequenced fungal genomes is ever increasing, with about 200 genomes already fully sequenced or in progress. Only a small percentage of those genomes have been comprehensively studied, for example using techniques from functional genomics. Comparative analysis has proven to be a useful strategy for enhancing our understanding of evolutionary biology and of the less well understood genomes. However, the data required for these analyses tends to be distributed in various heterogeneous data sources, making systematic comparative studies a cumbersome task. Furthermore, comparative analyses benefit from close integration of derived data sets that cluster genes or organisms in a way that eases the expression of requests that clarify points of similarity or difference between species. DESCRIPTION: To support systematic comparative analyses of fungal genomes we have developed the e-Fungi database, which integrates a variety of data for more than 30 fungal genomes. Publicly available genome data, functional annotations, and pathway information has been integrated into a single data repository and complemented with results of comparative analyses, such as MCL and OrthoMCL cluster analysis, and predictions of signaling proteins and the sub-cellular localisation of proteins. To access the data, a library of analysis tasks is available through a web interface. The analysis tasks are motivated by recent comparative genomics studies, and aim to support the study of evolutionary biology as well as community efforts for improving the annotation of genomes. Web services for each query are also available, enabling the tasks to be incorporated into workflows. CONCLUSION: The e-Fungi database provides fungal biologists with a resource for comparative studies of a large range of fungal genomes. Its analysis library supports the comparative study of genome data, functional annotation, and results of large scale analyses over all the genomes stored in the database. The database is accessible at http://www.e-fungi.org.uk, as is the WSDL for the web services.RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are

Distinct immune signatures in directly treated and distant tumors result from TLR adjuvants and focal ablation.

Both adjuvants and focal ablation can alter the local innate immune system and trigger a highly effective systemic response. Our goal is to determine the impact of these treatments on directly treated and distant disease and the mechanisms for the enhanced response obtained by combinatorial treatments. Methods: We combined RNA-sequencing, flow cytometry and TCR-sequencing to dissect the impact of immunotherapy and of immunotherapy combined with ablation on local and systemic immune components. Results: With administration of a toll-like receptor agonist agonist (CpG) alone or CpG combined with same-site ablation, we found dramatic differences between the local and distant tumor environments, where the directly treated tumors were skewed to high expression of F4/80, Cd11b and Tnf and the distant tumors to enhanced Cd11c, Cd3 and Ifng. When ablation was added to immunotherapy, 100% (n=20/20) of directly treated tumors and 90% (n=18/20) of distant tumors were responsive. Comparing the combined ablation-immunotherapy treatment to immunotherapy alone, we find three major mechanistic differences. First, while ablation alone enhanced intratumoral antigen cross-presentation (up to ~8% of CD45+ cells), systemic cross-presentation of tumor antigen remained low. Combining same-site ablation with CpG amplified cross-presentation in the draining lymph node (~16% of CD45+ cells) compared to the ablation-only (~0.1% of CD45+ cells) and immunotherapy-only cohorts (~10% of CD45+ cells). Macrophages and DCs process and present this antigen to CD8+ T-cells, increasing the number of unique T-cell receptor rearrangements in distant tumors. Second, type I interferon (IFN) release from tumor cells increased with the ablation-immunotherapy treatment as compared with ablation or immunotherapy alone. Type I IFN release is synergistic with toll-like receptor activation in enhancing cytokine and chemokine expression. Expression of genes associated with T-cell activation and stimulation (Eomes, Prf1 and Icos) was 27, 56 and 89-fold higher with ablation-immunotherapy treatment as compared to the no-treatment controls (and 12, 32 and 60-fold higher for immunotherapy-only treatment as compared to the no-treatment controls). Third, we found that the ablation-immunotherapy treatment polarized macrophages and dendritic cells towards a CD169 subset systemically, where CD169+ macrophages are an IFN-enhanced subpopulation associated with dead-cell antigen presentation. Conclusion: While the local and distant responses are distinct, CpG combined with ablative focal therapy drives a highly effective systemic immune response

CMOS image sensors for x-ray interferometry

X-ray interferometry (XRI) was first demonstrated in the early 2000’s, and many early mission concepts followed which exploited the significant improvement in spatial resolution that XRI offered. Unfortunately, optical technology was not mature enough to meet the requirements, and the idea remained dormant. ESA’s voyage 2050 programme, in combination with optical and pointing accuracy technology developments, has reignited interest in the concept, but large technological challenges still remain to realise such a groundbreaking telescope. Given the spectral and now spatial requirements of a XRI, the next generation of detector technologies must be developed which can meet those requirements to enable such a telescope. For the proposed ESA THESEUS x-ray astronomy mission, strict requirements on instrument operating temperature (-40°C) have necessitated developments of new detectors technologies, namely CMOS image sensors (CIS). The CEI, in collaboration with Te2v, have designed, manufactured, and characterised a monolithic fully depleted CIS specifically optimised for soft x-ray astronomy. The prototype detector currently meets the THESEUS soft x-ray imager requirements and boasts a near Fano-limited energy resolution of 130eV (@5.9keV) at -40°C. Although the new technology can perform well, the specific detector requirements of XRI need to balance opposing parameters of spatial and energy resolution. This paper will outline the current performance of the CIS221-X for soft x-ray astronomy (as well as other competing technologies) and describe future plans for developing CIS to meet the challenging requirements of XRI

The Role of Electron Captures in Chandrasekhar Mass Models for Type Ia Supernovae

The Chandrasekhar mass model for Type Ia Supernovae (SNe Ia) has received increasing support from recent comparisons of observations with light curve predictions and modeling of synthetic spectra. It explains SN Ia events via thermonuclear explosions of accreting white dwarfs in binary stellar systems, being caused by central carbon ignition when the white dwarf approaches the Chandrasekhar mass. As the electron gas in white dwarfs is degenerate, characterized by high Fermi energies for the high density regions in the center, electron capture on intermediate mass and Fe-group nuclei plays an important role in explosive burning. Electron capture affects the central electron fraction Y_e, which determines the composition of the ejecta from such explosions. Up to the present, astrophysical tabulations based on shell model matrix elements were only available for light nuclei in the sd-shell. Recently new Shell Model Monte Carlo (SMMC) and large-scale shell model diagonalization calculations have also been performed for pf-shell nuclei. These lead in general to a reduction of electron capture rates in comparison with previous, more phenomenological, approaches. Making use of these new shell model based rates, we present the first results for the composition of Fe-group nuclei produced in the central regions of SNe Ia and possible changes in the constraints on model parameters like ignition densities and burning front speeds.Comment: 26 pages, 8 figures, submitted to Ap