Industrial steam consumption analysis and prediction based on multi-source sensing data for sustainable energy development

Centralized heating is an energy-saving and environmentally friendly way that is strongly promoted by the state. It can improve energy utilization and reduce carbon emissions. However, Centralized heating depends on accurate heat demand forecasting. On the one hand, it is impossible to save energy if over producing, while on the other hand, it is impossible to meet the heat demand of enterprises if there is not enough capacity. Therefore, it is necessary to forecast the future trend of heat consumption, so as to provide a reliable basis for enterprises to reasonably deploy fuel stocks and boiler power. At the same time, it is also necessary to analyze and monitor the steam consumption of enterprises for abnormalities in order to monitor pipeline leakage and enterprise gas theft. Due to the nonlinear characteristics of heat load, it is difficult for traditional forecasting methods to capture data trend. Therefore, it is necessary to study the characteristics of heat loads and explore suitable heat load prediction models. In this paper, industrial steam consumption of a paper manufacturer is used as an example, and steam consumption data are periodically analyzed to study its time series characteristics; then steam consumption prediction models are established based on ARIMA model and LSTM neural network, respectively. The prediction work was carried out in minutes and hours, respectively. The experimental results show that the LSTM neural network has greater advantages in this steam consumption load prediction and can meet the needs of heat load prediction

Protelenomus Kieffer is a derived lineage of Trissolcus Ashmead (Hymenoptera, Scelionidae), with comments on the evolution of phoresy in Scelionidae

Species of the genus Protelenomus Kieffer (Platygastroidea, Scelionidae) are phoretic egg parasitoids of coreid bugs. The discovery, DNA sequencing, and molecular phylogenetic analysis of a Protelenomus species phoretic on Cletus punctiger (Dallas) (Hemiptera, Coreidae) shows that Protelenomus is a derived lineage of Trissolcus Ashmead. Protelenomus is treated as a junior synonym and a new species of phoretic Trissolcus, T. siliangae Yan, Chen & Talamas, is described from China

Isolation and characterization of porcine amniotic fluid-derived multipotent stem cells

The aim of this study was to isolate and characterize porcine amniotic fluid-derived multipotent stem cells (pAF-MSC). The porcine amniotic fluid (AF) from the amniotic cavity of pregnant gilts in the early stages of gestation (at E35) was collected and centrifuged for 5-10 min at 400 g to pellet cells. The primary culture of AF showed the multiple cell types, including the epithelial-like cells and fibroblast-like cells. By culturing in AMM medium for 6 to 8 days, the epithelial-like cells disappeared and the remaining cells presented the fibroblastoid morphology. The doubling time of pAF-MSCs was about 34.6 h, and the cells had been continually cultured over 60 passages in vitro. The flow cytometry results showed that pAF-MSCs were positive for CD44, CD117 and CD166, but negative for CD34, CD45 and CD54. Meanwhile, pAF-MSCs expressed ES cell markers, such as Oct4, Nanog, SSEA4, Tra-1-60 and Tra-1-81. The ratio of CD117(+) CD44(+) cells accounted for 98% of pAF-MSCs population. Three germ layer markers, including FGF5 (ectodermal marker), AFP (endodermal marker) and Bra (mesodermal marker), were detected in embryoid bodies derived from pAF-MSCs. Under the different induction conditions, the pAF-MSCs were capable of differentiating into neurocytes, adipocytes and beating cardiomyocytes. Furthermore, the pAF-MSCs didn't form teratoma when injected into immunodeficiency mice. These optimal features of pAF-MSCs provide an excellent alternative stem cell resource for potential cell therapy in regenerative medicine and transgenic animals

Anatomical basis for the choice of laparoscopic surgery for low rectal cancer through the pelvic imaging data—a cohort study

Abstract Background Low rectal cancer surgery without anus conservation needs permanent ileostomy or colostomy which seriously affects the quality of life of patients. Therefore, low rectal cancer surgery not only pays attention to the safety of surgical treatment but also to the anus conservation. Methods Sixty-seven patients suffering from low rectal cancer had undergone laparoscopic surgery which was analyzed through retrospective study. They were divided into the anus-conserving and non-anus-conserving groups. Thirty-five set of pelvic data was obtained from the preoperative CT and MRI images. After that, the discriminant function was obtained to predict the surgery methods for patients with low rectal carcinoma. Results Anal-conserving group discriminant function (F1) = − 33.698 + 6.045 × anal margin distance (cm) + 1.105 × T4; non-anus-conserving group discriminant function (F2) = − 14.125 + 3.138 × anal margin distance (cm) + 0.804 × T4. If F1 is greater than F2, then the case can be treated as the anus reservation while if F2 is greater than F1 the case cannot be treated anus reservation. The accuracy of the discriminant function was evaluated which was found to be 97%. Conclusion The discriminant function of pelvic data provides anatomical basis for the choice of surgical methods for low rectal cancer

Porcine induced pluripotent stem cells require LIF and maintain their developmental potential in early stage of embryos.

Porcine induced pluripotent stem (piPS) cell lines have been generated recently by using a cocktail of defined transcription factors, however, the features of authentic piPS cells have not been agreed upon and most of published iPS clones did not meet the stringent requirements of pluripotency. Here, we report the generation of piPS cells from fibroblasts using retrovirus carrying four mouse transcription factors (mOct4, mSox2, mKlf4 and mc-Myc, 4F). Multiple LIF-dependent piPS cell lines were generated and these cells showed the morphology similar to mouse embryonic stem cells and other pluripotent stem cells. In addition to the routine characterization, piPS cells were injected into porcine pre-compacted embryos to generate chimera embryos and nuclear transfer (NT) embryos. The results showed that piPS cells retain the ability to integrate into inner and outer layers of the blastocysts, and support the NT embryos development to blastocysts. The generations of chimera embryos and NT embryos derived from piPS clones are a practical means to determine the quality of iPS cells ex vivo

Lupeol Accumulation Correlates with Auxin in the Epidermis of Castor

Lupeol, a natural lupane-type pentacyclic triterpene, possesses various pharmacological properties, and its production attracts attention. Significant quantities of lupeol are deposited on the castor aerial organ surface and are easily extractable as a predominant wax constituent. Thus, castor might be considered as a potential bioreactor for the production of lupeol. The lupeol biosynthesis pathway is well known, but how it is regulated remains largely unknown. Among large numbers of castor cultivars, we targeted one accession line (337) with high levels of lupeol on its stem surface and low levels thereof on its hypocotyl surface, implicating that lupeol synthesis is differentially regulated in the two organs. To explore the underlying mechanisms, we did comparative transcriptome analysis of the first internode of 337 stem and the upper hypocotyl. Our results show that large amounts of auxin-related genes are differentially expressed in both parts, implying some possible interactions between auxin and lupeol production. We also found that several auxin-responsive cis-elements are present in promoter regions of HMGR and LUS genes encoding two key enzymes involved in lupeol production. Furthermore, auxin treatments apparently induced the expression levels of RcHMGR and RcLUS. Furthermore, we observed that auxin treatment significantly increased lupeol contents, whereas inhibiting auxin transport led to an opposite phenotype. Our study reveals some relationships between hormone activity and lupeol synthesis and might provide a promising way for improving lupeol yields in castor

Spatial-Temporal Changes and Driving Force Analysis of Green Space in Coastal Cities of Southeast China over the Past 20 Years

The purpose of this study is to reveal the spatial-temporal change and driving factors of green space in coastal cities of southeast China over the past 20 years. A supervised classification method combining support vector machines (SVMs) and visual interpretation was used to extract the green space from Landsat TM/OLI imageries from 2000–2020. The landscape pattern index was used to calculate geospatial information of green space and analyze their spatial-temporal changes. The hierarchical partitioning analysis was then used to determine the influences of anthropogenic and geographic environmental factors on the spatial-temporal changes in green space. The results indicated that the total area of green space remained constant over the past 20 years in coastal cities of southeast China (1% reduction). The spatial change of green space mainly occurred in the area near the ocean and the southern region. 41.37% of forest land was transferred from cultivated land, while 44.56%, 41.83%, 43.20%, 46.31%, 41.98% and 40.20% of shrub land, sparse woodland, other woodland, high-coverage grassland, moderate-coverage grassland and low-coverage grassland were transferred from forest land. The number of patches, patch density, edge density, landscape shape index and Shannon’s diversity index increased from 2000–2015, and then decreased to the minimum in 2020, while largest patch index continued to decline from 2000–2020. The contribution of anthropogenic factors (0.53–0.61) on the spatial-temporal changes of green space continually increased over the past 20 years, which was also higher than geographical environment factors (0.39–0.41). Our study provides a new perspective to distinguish the impact of anthropogenic activities and geographical environmental factors on the change of green space area, thereby providing a theoretical support for the construction and ecological management of green space

Characterization of piPS cells.

<p>A, The immunostaining of piPS cell line PS24 at passage 10. The nuclei were stained with Hoechst 33342 (Hoe). Scale bars, 100 µm. B, Quantitative RT-PCR analysis of expressions of porcine pluripotent factors (pOCT4, pSOX2, pNANOG and pTERT) in three piPS lines (PS23, PS24, PS31) and PEF. y axis represents the fold change relative to β-actin. C, Semi-quantitative RT-PCR analysis of the transgene expression (mOct4, mSox2, mKlf4, mc-Myc) in three piPS lines. iPEF, PEF cells were infected by 4F for 6 days. GAPDH was used as an internal control. D, The DNA methylation analysis of OCT4 promoter region in PS24 cell line and PEF by bisulfite sequencing. Open circles indicate unmethylated CpG, and filled circles indicate methylated CpG. E, Embryoid bodies derived from PS24 line were cultured in suspension for 5 days (a), and then cultured in tissue culture plat for the differentiation (b). Scale bars, 200 µm. F, The quantitative RT-PCR analysis of PS24 clone that was differentiate into three germ layers, NESTIN for ectoderm, DES for mesoderm and NCSTN for endoderm. The upper panels show the spontaneous differentiation for 0, 5 and 10 days, and the lower panels show the induced differentiation by RA for 0, 5 and 10 days. The y axis represents the fold change vs. GAPDH. G, Hematoxylin-eosin stained tissue section of teratoma derived from piPS clone PS24. Cells were transplanted subcutaneously in CB-17 SCID mice for 8 weeks. The teratoma and tissues representing three germ layers, neural epithelium (ectoderm), muscle (mesoderm) and gut epithelium (endoderm), are indicated by arrows. Scale bars, 100 µm. H, The heat map shows the single-linkage hierarchical clustering of microarray data (n = 20640 probes) for 2 piPS cell lines (PS24 and 30AC5) and PFF. The relative abundance of gene expression was clustered by Euclidean correlation and complete linkage. Data indicate mean ± SD (n = 3). Different letters (a, b, c) indicate significantly different between two groups, <i>p</i><0.01 by Student’s <i>t</i> test.</p

Expression of SSEA4 during the process of PEF reprogramming.

<p>The pMXs-GFP was co-transfected with mouse 4F into PEF cells. The immunofluorescence assay of SSEA4 was conducted in different days, and the cell populations were sorted by the flow cytometer (right panel). A, At 8 days post-infection; B, At 12 days post-infection; C, At 16 days post-infection. Hoe, Hoechst 33342. Scale bars, 200 µm for a–b; 100 µm for c.</p