360 research outputs found
Probabilistic inference of fatigue damage propagation with limited and partial information
AbstractA general method of probabilistic fatigue damage prognostics using limited and partial information is developed. Limited and partial information refers to measurable data that are not enough or cannot directly be used to statistically identify model parameter using traditional regression analysis. In the proposed method, the prior probability distribution of model parameters is derived based on the principle of maximum entropy (MaxEnt) using the limited and partial information as constraints. The posterior distribution is formulated using the principle of maximum relative entropy (MRE) to perform probability updating when new information is available and reduces uncertainty in prognosis results. It is shown that the posterior distribution is equivalent to a Bayesian posterior when the new information used for updating is point measurements. A numerical quadrature interpolating method is used to calculate the asymptotic approximation for the prior distribution. Once the prior is obtained, subsequent measurement data are used to perform updating using Markov chain Monte Carlo (MCMC) simulations. Fatigue crack prognosis problems with experimental data are presented for demonstration and validation
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Steric trapping reveals a cooperativity network in the intramembrane protease GlpG.
Membrane proteins are assembled through balanced interactions among proteins, lipids and water. Studying their folding while maintaining the native lipid environment is necessary but challenging. Here we present methods for analyzing key elements of membrane protein folding including thermodynamic stability, compactness of the unfolded state and folding cooperativity under native conditions. The methods are based on steric trapping, which couples the unfolding of a doubly biotinylated protein to the binding of monovalent streptavidin (mSA). We further advanced this technology for general application by developing versatile biotin probes possessing spectroscopic reporters that are sensitized by mSA binding or protein unfolding. By applying these methods to the Escherichia coli intramembrane protease GlpG, we elucidated a widely unraveled unfolded state, subglobal unfolding of the region encompassing the active site, and a network of cooperative and localized interactions to maintain stability. These findings provide crucial insights into the folding energy landscape of membrane proteins
Torque Enhancement of Dual Three-Phase PMSM by Harmonic Injection
The torque enhancement of dual three-phase permanent magnet synchronous machine (DT-PMSM) drive system by full exploitation of flux-linkage and current harmonics are comparatively studied in this paper. The torque capability of DT-PMSM is previously evaluated with strategies of harmonics utilization, i.e. Strategy-1 of 3rd harmonic utilization and Strategy-2 of 5th and 7th harmonic utilization, which can extend the torque capability by 18.2% and 9.0% respectively. However, the full exploitation of harmonics including 3rd, 5th and 7th harmonics in the dual three-phase system are not addressed. In this paper, the Strategy-3 of 3rd, 5th and 7th harmonic utilization is also included. Its corresponding harmonic current control is proposed and the average torque and harmonic torque are analyzed in detail. Based on a test rig with existing prototype DT-PMSM, the torque with Strategy-3 is increased up to 26.5%, which is superior to the previous strategies
Design and synthesis of active heparan sulfate-based probes
A chemoenzymatic approach for synthesizing heparan sulfate oligosaccharides with a reactive diazoacetyl saccharide residue is reported. The resultant oligosaccharides were demonstrated to serve as specific inhibitors for heparan sulfate sulfotransferases, offering a new set of tools to probe the structural selectivity for heparan sulfate-binding proteins
Flux-Weakening Control of Dual Three-Phase PMSM Based on Vector Space Decomposition Control
This paper proposes a flux-weakening (FW) control for dual three-phase ermanent magnet synchronous machine (DT-PMSM) based on vector space decomposition (VSD) control, where the output voltage in αβ sub-plane is employed for voltage feedback in the flux-weakening control loop. As the fundamental components are mapped to αβ sub-plane while the 5th and 7th harmonics are projected to harmonic z1z2 sub-plane, the flux-weakening current from this new control in αβ sub-plane is sixth harmonic-free regardless of the 5th and 7th harmonics being resulted from the non-sinusoidal back EMF or inverter non-linearity. The proposed control is compared with the conventional FW feedback control extended for DT-PMSM, where the FW control is applied to the two sets of three-phase windings separately. The experimental results show that the proposed FW control based on VSD is superior to the conventional FW control in terms of reduction in current unbalance and harmonic currents
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