PRELIMINARY DESIGN AND FEASIBILITY STUDY FOR THE CONSTRUCTION OF THE SECOND BLOCK THERMAL POWER PLANT PLJEVLJA

V magisterski nalogi smo predstavili možnost gradnje drugega bloka termoelektrarne v občini Pljevlja. Z gradnjo novega bloka TE Pljevlja želimo pokriti potrebe po električni energiji v Črni Gori in zmanjšati negativni vpliv na okolje obstoječe termoelektrarne. Predstavili smo tudi oceno vrednosti investicije za novo enoto v okviru obstoječe TE.  In this paper we present the possibility for construction of the second block thermal power plant in the municipality of Pljevlja. With the construction of a new block TPP Pljevlja we want to cover electricity demand in Montenegro and reducing the negative environmental impact of existing thermal power plants. We also presented an assessment of the value of the investment for a new unit within the existing thermal power plants

Деградація земель у Калуському районі внаслідок сольового забруднення

Показано, що джерелами деградації ґрунтів внаслідок, їх засолення, є солевідвали Домбровського кар’єру. Основними чинниками, що призводять до деградації є вітрова і водна ерозія. Досліджено, що основну роль в засоленні ґрунтового покриву відіграють процеси дифузії. Встановлено, що площа засолення (деградація) ґрунтів у декілька разів перевищує площу солевідводів.Показано, что источниками деградации почв вследствие их засоления, являются солеотвалы Домбровского карьера. Основными факторами, которые приводят к деградации является ветровая и водная эрозия. Доказано, что основную роль в засолении почвенного покрова играют процессы диффузии. Установлено, что площадь засоления (деградация) почв в несколько раз превышает площадь солеотвалов.In the article is shown that the sources of land degradation occurs because of their salinity and salt piles from Dombrowsky career. The main factors that lead to the degradation are wind and water erosion. It is investigated that the main role in the salinity of soil processes play diffusion. It is established that the area of salinity (degradation) of soil several times salt piles are

Additional file 2: of Recalcitrant carbon controls the magnitude of soil organic matter mineralization in temperate forests of northern China

Raw data for mineralized SOC during a given incubation period under different conditions for three forest types. (DOCX 27 kb

Alignments of the DinoSL-containing cDNAs obtained in this study (DinoSL) with their corresponding genomic (#) or cDNA sequences (##) reported previously [7], [9].

<p>Exons are shown in upper case while introns in lower case; consensus positions are denoted by asterisks. The 22-nt DinoSL was underlined. Note that in all cases, the previously reported sequences missed varying lengths of sequences at the 5′-end.</p

Primers used in this study.

<p>Primers used in this study.</p

Additional file 1: of Recalcitrant carbon controls the magnitude of soil organic matter mineralization in temperate forests of northern China

Labile carbon pool and decomposition constants estimated by a two-compartment modelunder different incbuation conditions for three forest types. (DOC 54 kb

<i>Amphidinium carterae</i> gene transcripts previously reported to lack DinoSL and the corresponding cDNAs with DinoSL obtained in our laboratory.

a<p>Bachvaroff and Place 2008 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0004129#pone.0004129-Bachvaroff1" target="_blank">[7]</a>.</p>b<p>Zhang et al. 2007 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0004129#pone.0004129-Zhang1" target="_blank">[1]</a>.</p>c<p>This study.</p

Light-Promoted Rhodopsin Expression and Starvation Survival in the Marine Dinoflagellate <i>Oxyrrhis marina</i>

<div><p>The discovery of microbial rhodopsins in marine proteobacteria changed the dogma that photosynthesis is the only pathway to use the solar energy for biological utilization in the marine environment. Although homologs of these rhodopsins have been identified in dinoflagellates, the diversity of the encoding genes and their physiological roles remain unexplored. As an initial step toward addressing the gap, we conducted high-throughput transcriptome sequencing on <i>Oxyrrhis marina</i> to retrieve rhodopsin transcripts, rapid amplification of cDNA ends to isolate full-length cDNAs of dominant representatives, and quantitative reverse-transcription PCR to investigate their expression under varying conditions. Our phylogenetic analyses showed that <i>O</i>. <i>marina</i> contained both the proton-pumping type (PR) and sensory type (SR) rhodopsins, and the transcriptome data showed that the PR type dominated over the SR type. We compared rhodopsin gene expression for cultures kept under light: dark cycle and continuous darkness in a time course of 24 days without feeding. Although both types of rhodopsin were expressed under the two conditions, the expression levels of PR were much higher than SR, consistent with the transcriptomic data. Furthermore, relative to cultures kept in the dark, rhodopsin expression levels and cell survival rate were both higher in cultures grown in the light. This is the first report of light-dependent promotion of starvation survival and concomitant promotion of PR expression in a eukaryote. While direct evidence needs to come from functional test on rhodopsins <i>in vitro</i> or gene knockout/knockdown experiments, our results suggest that the proton-pumping rhodopsin might be responsible for the light-enhanced survival of <i>O</i>. <i>marina</i>, as previously demonstrated in bacteria.</p></div

Tandem Repeats, High Copy Number and Remarkable Diel Expression Rhythm of Form II RuBisCO in <i>Prorocentrum donghaiense</i> (Dinophyceae)

<div><p>Gene structure and expression regulation of form II RuBisCO (<i>rbcII</i>) in dinoflagellates are still poorly understood. Here we isolated this gene (<i>Pdrbc</i>) and investigated its diel expression pattern in a harmful algal bloom forming dinoflagellate <i>Prorocentrum donghaiense</i>. We obtained cDNA sequences with triple tandem repeats of the coding unit (CU); the 5′ region has the sequence of a typical dinoflagellate plastid gene, encoding an N-terminus with two transmembrane regions separated by a plastid transit peptide. The CUs (1,455 bp except 1464 bp in last CU) are connected through a 63 bp spacer. Phylogenetic analysis showed that <i>rbcII</i> CUs within species formed monophyletic clusters, indicative of intraspecific gene duplication or purifying evolution. Using quantitative PCR (qPCR) we estimated 117±40 CUs of <i>Pdrbc</i> in the <i>P. donghaiense</i> genome. Although it is commonly believed that most dinoflagellate genes lack transcriptional regulation, our RT-qPCR analysis on synchronized cultures revealed remarkable diel rhythm of <i>Pdrbc</i> expression, showing significant correlations of transcript abundance with the timing of the dark-to-light transition and cell cycle G2M-phase. When the cultures were shifted to continuous light, <i>Pdrbc</i> expression remained significantly correlated with the G2M-phase. Under continuous darkness the cell cycle was arrested at the G1 phase, and the rhythm of <i>Pdrbc</i> transcription disappeared. Our results suggest that dinoflagellate <i>rbcII</i> 1) undergoes duplication or sequence purification within species, 2) is organized in tandem arrays in most species probably to facilitate efficient translation and import of the encoded enzyme, and 3) is regulated transcriptionally in a cell cycle-dependent fashion at least in some dinoflagellates.</p></div

Primers used in this study.

<p>Primers used in this study.</p