Analysis of Effect of Schisandra in the Treatment of Myocardial Infarction Based on Three-Mode Gene Ontology Network

Schisandra chinensis is a commonly used traditional Chinese medicine, which has been widely used in the treatment of acute myocardial infarction in China. However, it has been difficult to systematically clarify the major pharmacological effect of Schisandra, due to its multi-component complex mechanism. In order to solve this problem, a comprehensive network analysis method was established based-on “component–gene ontology–effect” interactions. Through the network analysis, reduction of cardiac preload and myocardial contractility was shown to be the major effect of Schisandra components, which was further experimentally validated. In addition, the expression of NCOR2 and NFAT in myocyte were experimentally confirmed to be associated with Schisandra in the treatment of AMI, which may be responsible for the preservation effect of myocardial contractility. In conclusion, the three-mode gene ontology network can be an effective network analysis workflow to evaluate the pharmacological effects of a multi-drug complex system

Changes in aroma composition of blackberry wine during fermentation process

The study aimed at investigating the influence of fermentation (primary and secondary) on aroma composition of blackberry wine. Gas chromatography-mass spectrometry (GC-MS) was applied to quantify the compounds relevant to sparkling wine aroma. Investigation on this study revealed that a number of aroma components in raw material (55 in numbers), raw wine (54 in numbers), and aging wine (50 in numbers) were identified. In addition, 9 new aroma components such as octanoate, benzenepropanoic acid ethyl ester, ethyl benzoate, dodecyl ethyl, n-propanol, n-butanol, d-citronellol, benzaldehyde, and cedrol were detected in natural aging wine which appeared during secondary fermentation according to total peak areas of 4.69%. These findings reveal that natural aging is very important to aroma components formation of blackberry wine.Key words: Blackberry, gas chromatography, primary fermentation, secondary fermentation

Efficient induction of CD25- iTreg by co-immunization requires strongly antigenic epitopes for T cells

Background: We previously showed that co-immunization with a protein antigen and a DNA vaccine coding for the same antigen induces CD40(low) IL-10(high) tolerogenic DCs, which in turn stimulates the expansion of antigenspecific CD4(+)CD25(-)Foxp3(+) regulatory T cells (CD25(-) iTreg). However, it was unclear how to choose the antigen sequence to maximize tolerogenic antigen presentation and, consequently, CD25(-) iTreg induction. Results: In the present study, we demonstrated the requirement of highly antigenic epitopes for CD25(-) iTreg induction. Firstly, we showed that the induction of CD25(-) iTreg by tolerogenic DC can be blocked by anti-MHC-II antibody. Next, both the number and the suppressive activity of CD25(-) iTreg correlated positively with the overt antigenicity of an epitope to activate T cells. Finally, in a mouse model of dermatitis, highly antigenic epitopes derived from a flea allergen not only induced more CD25(-) iTreg, but also more effectively prevented allergenic reaction to the allergen than did weakly antigenic epitopes. Conclusions: Our data thus indicate that efficient induction of CD25- iTreg requires highly antigenic peptide epitopes. This finding suggests that highly antigenic epitopes should be used for efficient induction of CD25- iTreg for clinical applications such as flea allergic dermatitis

Effect of Vacuum Pre-cooling on the Circulation and Shelf Quality of Postharvest Baby Cabbage

To optimize the treatment conditions for vacuum pre-cooling of baby cabbage and maintain the fresh quality of postharvest baby cabbage, a central composite response surface experiment with two factors and three levels was conducted on the basis of analyzing the effects of different vacuum pre-cooling final temperature (0, 2 and 4 ℃) and different vacuum pre-cooling final pressure (400, 600, 800 and 1000 Pa) on the pre-cooling of postharvest baby cabbage. The results showed that compared with the control without pre-cooling and the pre-cooling group with final temperature of 0 ℃, the pre-cooling with final temperature of 2~4 ℃ at the final pressure of 600 Pa could better maintain the appearance quality of postharvest baby cabbage, inhibit the increase of respiratory rate and malondialdehyde (MDA) content. Under the condition of final temperature of 2 ℃, compared with the control without pre-cooling and the pre-cooling group with a final pressure of 1000 Pa, pre-cooling with final pressures of 400~800 Pa could also better maintain the appearance quality of postharvest baby cabbage, inhibit the increase of respiratory rate and MDA content. Further central composite response surface experiments showed that the optimal conditions for vacuum pre-cooling of postharvest baby vegetables were final temperature of 4.0 ℃ and final pressure of 600 Pa. The vacuum pre-cooling condition not only maintained good quality of postharvest baby vegetable, but also increased the content of soluble protein, total phenols, and ascorbic acid by 18.78%, 18.47% and 33.23%, respectively. Therefore, appropriate vacuum pre-cooling treatment is a highly potential commercialization technology that could effectively maintain the good quality of postharvest baby vegetable during circulation and shelf life

Effects of Ultrasonic Atomization of Slightly Acidic Electrolyzed Water on the Circulation and Shelf Quality of Postharvest Baby Cabbage

To check the effect of slightly acidic electrolyzed water (SAEW) on the shelf quality of postharvest baby cabbage, the effects of ultrasonic atomization fumigation of SAEW with different concentrations (0, 50, 100 and 150 mg/L) on the postharvest preservation of baby cabbage were analyzed under low temperature circulation (4±1) ℃and shelf conditions (25±1) ℃. The results showed that, compared with the control and other concentrations of SAEW (50 and 150 mg/L), the ultrasonic atomization fumigation treatment of SAEW at 100 mg/L treatment significantly suppressed the increase in malondialdehyde content and the decrease in total glucosinolate content of baby cabbage at the 6th and 9th days of shelf life (P<0.05). Further circulation and shelf simulation results indicated that, compared with the control, the 100 mg/L SAEW ultrasonic atomization fumigation treatment reduced the total number of colonies by 17.04% and increased the total glucosinolate content by 30.11% in baby cabbage; In addition, this treatment significantly inhibited the accumulation of nitrite and malondialdehyde content (P<0.05), delayed the decline of soluble sugar, soluble protein, total phenol, ascorbic acid and isothiocyanate content, and increased the activity of myrosinase in postharvest baby cabbage. It was clear that ultrasonic atomization fumigation treatment of SAEW at 100 mg/L could not only effectively inhibit the growth of the total number of colonies in baby cabbage during circulation and shelf life, but also effectively slow down the degradation of nutritional quality in baby cabbage during this process, thus improving the preservation effect of baby cabbage

Effect of Slightly Acidic Electrolyzed Water Treatment on Microbial Growth Inhibition and Storage Quality of Postharvest Chives

To explore the effect of slightly acidic electrolyzed water (SAEW) on the storage quality of postharvest chives, the changes in the microbial load and storage quality of postharvest chives subjected to SAEW immersion treatment at different concentrations (0, 100, 200 and 300 mg/L) for 5 minutes were analyzed during low temperature ((4 ± 1) ℃) storage. The results showed that immersion treatment with 200 mg/L SAEW was more effective in maintaining the organoleptic quality of chives and inhibiting the increase of the total number of colonies when compared with the other groups. Furthermore, 200 mg/L SAEW treatment was able to inhibit the increase of mass loss and rot rate, slow down the decrease of chlorophyll, vitamin C, soluble sugar, and soluble protein contents, and reduce the accumulation of total free amino acids, malondialdehyde (MDA) and nitrite in chives. It is evident that immersion treatment with 200 mg/L SAEW can not only effectively inhibit microbial growth on chives during low-temperature storage, but also effectively delay the loss of nutrients in chives, thus improving the quality preservation of chives. Therefore, SAEW can be used as an effective sterilization and preservation method to maintain the storage quality and delay the aging process of postharvest chives

Effect of Slightly Acidic Electrolyzed Water on Chlorophyll Degradation in Postharvest Broccoli

To explore the effect of slightly acidic electrolyzed water (SAEW) on chlorophyll degradation in postharvest broccoli, the pattern of changes in the color, total chlorophyll content, chlorophyll derivative content, chlorophyll degrading enzyme activities, and key chlorophyll metabolism-related gene expression of postharvest broccoli after treatment with 50 mg/L SAEW was analyzed. The results showed that SAEW treatment could effectively slow down the degradation of total chlorophyll, maintain the contents of chlorophyll derivatives chlorophyll a, chlorophyll b, chlorophyllide a, chlorophyllide b, pheophorbide a, and pheophytin a, and delay the increase in the activities of chlorophyll metabolizing enzymes, Mg-dechelatase, pheophytinase, and pheophorbide an oxygenase in postharvest broccoli. Meanwhile, it significantly inhibited the expression of the genes encoding chlorophyll b reductase, chlorophyllase 1, chlorophyllase 2, chlorosis protein, pheophytinase, pheophorbide an oxygenase, red chlorophyll catabolite reductase, and aging specific cysteine protease, thereby allowing color protection and freshness preservation. In conclusion, SAEW can be used as an effective method to delay postharvest chlorophyll degradation and inhibit yellowing and senescence in broccoli

Quasi-Two-Dimensional Fermi Surface and Heavy Quasiparticles in CeRh2As2

The recent discovery of multiple superconducting phases in CeRh2As2 has attracted considerable interest. These rich phases are thought to be related to the locally noncentrosymmetric crystal structure, although the possible role of a quadrupole density wave preceding the superconductivity remains an open question. While measurements of physical properties imply that the Ce 4f electrons could play an essential role, the momentum-resolved electronic structure remains hitherto unreported, hindering an in-depth understanding of the underlying physics. Here, we report a high-resolution angle-resolved photoemission study of CeRh2As2. Our results reveal fine splittings of conduction bands, which are directly related to the locally noncentrosymmetric structure, as well as a quasi-two-dimensional Fermi surface, implying weak interlayer hopping and possible nesting instabilities. Our experiments also uncover the fine structures and pronounced temperature evolution of the Kondo peak, demonstrating strong Kondo effect facilitated by excited crystal electric field states. Our results unveil the salient electronic features arising from the interplay between the crystal structure and strong electron correlation, providing spectroscopic insight for understanding the heavy fermion physics and unconventional quadrupole density wave in this enigmatic compound

Coherent control of a high-orbital hole in a semiconductor quantum dot

Coherently driven semiconductor quantum dots are one of the most promising platforms for non-classical light sources and quantum logic gates which form the foundation of photonic quantum technologies. However, to date, coherent manipulation of single charge carriers in quantum dots is limited mainly to their lowest orbital states. Ultrafast coherent control of high-orbital states is obstructed by the demand for tunable terahertz pulses. To break this constraint, we demonstrate an all-optical method to control high-orbital states of a hole via stimulated Auger process. The coherent nature of the Auger process is proved by Rabi oscillation and Ramsey interference. Harnessing this coherence further enables the investigation of single-hole relaxation mechanism. A hole relaxation time of 161 ps is observed and attributed to the phonon bottleneck effect. Our work opens new possibilities for understanding the fundamental properties of high-orbital states in quantum emitters and developing new types of orbital-based quantum photonic devices.Comment: Manuscript with 14 pages and 6 figures plus supplementary Information comprising 15 pages and 14 figure

Suppression of interferon α and γ response by Huwe1-mediated Miz1 degradation promotes SARS-CoV-2 replication

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has been demonstrated to limit the host interferon response; however, the underlying mechanism remains unclear. Here, we found that SARS-CoV-2 infection upregulated the E3 ubiquitin ligase Huwe1, which in turn facilitated the degradation of the transcription factor Miz1. The degradation of Miz1 hampered interferon alpha and gamma responses, consequently fostering viral replication and impeding viral clearance. Conversely, silencing or inhibiting Huwe1 enhanced the interferon responses, effectively curbing viral replication. Consistently, overexpressing Miz1 augmented the interferon responses and limited viral replication, whereas silencing Miz1 had the opposite effect. Targeting Huwe1 or overexpressing Miz1 elicited transcriptomic alterations characterized by enriched functions associated with bolstered antiviral response and diminished virus replication. Further study revealed Miz1 exerted epigenetic control over the transcription of specific interferon signaling molecules, which acted as common upstream regulators responsible for the observed transcriptomic changes following Huwe1 or Miz1 targeting. These findings underscore the critical role of the Huwe1-Miz1 axis in governing the host antiviral response, with its dysregulation contributing to the impaired interferon response observed during COVID-19