Multi-omics revealed rumen microbiota metabolism and host immune regulation in Tibetan sheep of different ages

The rumen microbiota and metabolites play an important role in energy metabolism and immune regulation of the host. However, the regulatory mechanism of rumen microbiota and metabolite interactions with host on Tibetan sheep’s plateau adaptability is still unclear. We analyzed the ruminal microbiome and metabolome, host transcriptome and serum metabolome characteristics of Tibetan sheep at different ages. Biomarkers Butyrivibrio, Lachnospiraceae_XPB1014_group, Prevotella, and Rikenellaceae_RC9_gut_group were found in 4 months, 1.5 years, 3.5 years, and 6 years Tibetan sheep, respectively. The rumen microbial metabolites were mainly enriched in galactose metabolism, unsaturated fatty acid biosynthesis and fatty acid degradation pathways, and had significant correlation with microbiota. These metabolites further interact with mRNA, and are co-enriched in arginine and proline metabolism, metabolism of xenobiotics by cytochrome P450, propanoate metabolism, starch and sucrose metabolism, gap junction pathway. Meanwhile, serum metabolites also have a similar function, such as chemical carcinogenesis − reactive oxygen species, limonene and pinene degradation, and cutin, suberine and wax biosynthesis, thus participating in the regulation of the body’s immune and energy-related metabolic processes. This study systematically revealed that rumen microbiota, metabolites, mRNA and serum metabolites of Tibetan sheep were involved in the regulation of fermentation metabolic function and immune level of Tibetan sheep at different ages, which provided a new perspective for plateau adaptability research of Tibetan sheep at different ages

Deregulation of Regulatory T Cells in Acute-on-Chronic Liver Failure: A Rat Model

Aims. Acute-on-chronic liver failure (ACLF) and acute liver failure (ALF) are similar in many respects during their acute exacerbation; however, ACLF generally has a poorer prognosis. We aimed to investigate the role and dynamic changes of regulatory T cell (Treg) and T helper 17 (Th17) cell proportions during ACLF progress. Methods. All rats were classified into two groups randomly: ACLF group and ALF group (control group). The rat model of ACLF was preestablished by intraperitoneal injection of carbon tetrachloride for 2 months. Then acute liver injury was induced by combined D-galactosamine and lipopolysaccharide. Six time points were examined before or after acute induction. Liver samples were performed with hematoxylin-eosin and Masson staining; circulatory Treg and Th17 cell frequencies were determined using flow cytometry assays; serum levels of alanine aminotransferase, aspartate aminotransferase, interleukin-10 (IL-10), and interferon-γ (IFN-γ) were examined. Results. In group ACLF, both Th17 cell proportion and IFN-γ level presented upgrade firstly and then descend latter tendency; the trends of Treg cell proportion and IL-10 level were observed to gradually decrease and became stable. Conclusion. The Treg cells played an important role in the immunologic mechanism during the process of ACLF. And the function of Treg cells in ACLF was defective

Accurate evaluation of the kk-th derivative of a polynomial

International audienceThis paper presents a compensated algorithm for the evaluation of the k-th derivative of a polynomial in power basis. The proposed algorithm makes it possible the direct evaluation without obtaining the k-th derivative expression of the polynomial itself, with a very accurate result to all but the most ill-conditioned evaluation. Forward error analysis and running error analysis are performed by an approach based on the data dependency graph. Numerical experiments illustrate the accuracy and efficiency of the algorithm

Intelligent indoor metasurface robotics

International audienceIntelligent indoor robotics is expected to rapidly gain importance in crucial areas of our modern society such as at-home health care and factories. Yet, existing mobile robots are limited in their ability to perceive and respond to dynamically evolving complex indoor environments because of their inherently limited sensing and computing resources that are, moreover, traded off against their cruise time and payload. To address these formidable challenges, here we propose intelligent indoor metasurface robotics (I2MR), where all sensing and computing are relegated to a centralized robotic brain endowed with microwave perception; and I2MR’s limbs (motorized vehicles, airborne drones, etc.) merely execute the wirelessly received instructions from the brain. The key aspect of our concept is the centralized use of a computation-enabled programmable metasurface that can flexibly mold microwave propagation in the indoor wireless environment, including a sensing and localization modality based on configurational diversity and a communication modality to establish a preferential high-capacity wireless link between the I2MR’s brain and limbs. The metasurface-enhanced microwave perception is capable of realizing low-latency and high-resolution three-dimensional imaging of humans, even around corners and behind thick concrete walls, which is the basis for action decisions of the I2MR’s brain. I2MR is thus endowed with real-time and full-context awareness of its operating indoor environment. We implement, experimentally, a proof-of-principle demonstration at ∼2.4 GHz, in which I2MR provides health-care assistance to a human inhabitant. The presented strategy opens a new avenue for the conception of smart and wirelessly networked indoor robotics

Microwave Speech Recognizer Empowered by a Programmable Metasurface

International audienceSpeech recognition becomes increasingly important in the modern society, especially for human–machine interactions, but its deployment is still severely thwarted by the struggle of machines to recognize voiced commands in challenging real‐life settings: oftentimes, ambient noise drowns the acoustic sound signals, and walls, face masks or other obstacles hide the mouth motion from optical sensors. To address these formidable challenges, an experimental prototype of a microwave speech recognizer empowered by programmable metasurface is presented here that can remotely recognize human voice commands and speaker identities even in noisy environments and if the speaker's mouth is hidden behind a wall or face mask. The programmable metasurface is the pivotal hardware ingredient of the system because its large aperture and huge number of degrees of freedom allows the system to perform a complex sequence of sensing tasks, orchestrated by artificial‐intelligence tools. Relying solely on microwave data, the system avoids visual privacy infringements. The developed microwave speech recognizer can enable privacy‐respecting voice‐commanded human–machine interactions is experimentally demonstrated in many important but to‐date inaccessible application scenarios. The presented strategy will unlock new possibilities and have expectations for future smart homes, ambient‐assisted health monitoring, as well as intelligent surveillance and security

Rumen Epithelial Development- and Metabolism-Related Genes Regulate Their Micromorphology and VFAs Mediating Plateau Adaptability at Different Ages in Tibetan Sheep

The rumen is an important hallmark organ of ruminants and plays an important role in the metabolism and immune barrier of Tibetan sheep on the Plateau. However, there are few studies on rumen development and metabolism regulation in Tibetan sheep at different ages. Here, we comprehensively analyzed the immune function, fermentation function, rumen epithelial micromorphology and transcriptome profile of Tibetan sheep at different ages. The results showed that the concentration of IgG decreased and the concentration of IgM increased with age (p < 0.05), and the highest concentration of IgA was observed at 1.5 and 3.5 years of age. In terms of rumen fermentation characteristics, VFAs of 4-month-old lambs were the highest, followed by VFAs and NH3-N of Tibetan sheep at 3.5 years of age. Hematoxylin-eosin staining and transmission electron microscopy section examination of rumen epithelial tissue showed that the rumen papilla width increased with age (p < 0.001), the thickness of the stratum corneum decreased, the cells in the stratum corneum showed accelerated migration and the thickness of the rumen muscle layer increased (p < 0.001). Desmosomal junctions between the layers of rumen epithelium increased at 1.5 and 3.5 years old, forming a compact barrier structure, and the basal layer had more mitochondria involved in the regulation of energy metabolism. RNA-seq analysis revealed that a total of 1006 differentially expressed genes (DEGs) were identified at four ages. The DEGs of Tibetan sheep aged 4 months and 6 years were mainly enriched in the oxidation–reduction process and ISG15-protein conjugation pathway. The 1.5 and 3.5-year-olds were mainly enriched in skeletal muscle thin filament assembly, mesenchyme migration and the tight junction pathway. WGCNA showed that DEGs related to rumen microbiota metabolite VFAs and epithelial morphology were enriched in “Metabolism of xenobiotics by cytochrome P450, PPAR signaling pathway, Butanoate metabolism pathways” and participated in the regulation of rumen epithelial immune and fermentation metabolism functions of Tibetan sheep at different ages. This study systematically revealed the regulatory mechanism of rumen epithelial development and metabolism in the plateau adaptation of Tibetan sheep, providing a new approach for the study of plateau adaptation

Microwave Speech Recognizer Empowered by a Programmable Metasurface

We present an experimental prototype of a microwave speech recognizer empowered by a programmable metasurface that can recognize voice commands and speaker identities remotely even in noisy environments and if the speaker’s mouth is hidden behind a wall or face mask. Thereby, we enable voice-commanded human machine interactions in many important but to-date inaccessible application scenarios, including smart health careand factory scenarios. The programmable metasurface is the pivotal hardware ingredient of our system because its large aperture and huge number of degrees of freedom allows our system to perform a complex sequence of tasks, orchestrated by artificial-intelligence tools. First, the speaker’s mouth is localized by imaging the scene and identifying the region of interest. Second, microwaves are efficiently focused on the speaker’s mouth toencode information about the vocalized speech in reflected microwave biosignals. The efficient focusing on the speaker’s mouth is the origin of our system’s robustness to various types of parasitic motion. Third, a dedicated neural network directly retrieves the sought-after speech information from the measured microwave biosignals. Relying solely on microwave data, our system avoids visual privacy infringements. We expect that thepresented strategy will unlock new possibilities for future smart homes, ambient-assisted health monitoring and care, smart factories, as well as intelligent surveillance and security

Table_1_Multi-omics revealed rumen microbiota metabolism and host immune regulation in Tibetan sheep of different ages.XLSX

The rumen microbiota and metabolites play an important role in energy metabolism and immune regulation of the host. However, the regulatory mechanism of rumen microbiota and metabolite interactions with host on Tibetan sheep’s plateau adaptability is still unclear. We analyzed the ruminal microbiome and metabolome, host transcriptome and serum metabolome characteristics of Tibetan sheep at different ages. Biomarkers Butyrivibrio, Lachnospiraceae_XPB1014_group, Prevotella, and Rikenellaceae_RC9_gut_group were found in 4 months, 1.5 years, 3.5 years, and 6 years Tibetan sheep, respectively. The rumen microbial metabolites were mainly enriched in galactose metabolism, unsaturated fatty acid biosynthesis and fatty acid degradation pathways, and had significant correlation with microbiota. These metabolites further interact with mRNA, and are co-enriched in arginine and proline metabolism, metabolism of xenobiotics by cytochrome P450, propanoate metabolism, starch and sucrose metabolism, gap junction pathway. Meanwhile, serum metabolites also have a similar function, such as chemical carcinogenesis − reactive oxygen species, limonene and pinene degradation, and cutin, suberine and wax biosynthesis, thus participating in the regulation of the body’s immune and energy-related metabolic processes. This study systematically revealed that rumen microbiota, metabolites, mRNA and serum metabolites of Tibetan sheep were involved in the regulation of fermentation metabolic function and immune level of Tibetan sheep at different ages, which provided a new perspective for plateau adaptability research of Tibetan sheep at different ages.</p