Conservation genetics and phylogeography of endangered and endemic shrub Tetraena mongolica (Zygophyllaceae) in Inner Mongolia, China

<p>Abstract</p> <p>Background</p> <p><it>Tetraena mongolica </it>(Zygophyllaceae), an endangered endemic species in western Inner Mongolia, China. For endemic species with a limited geographical range and declining populations, historical patterns of demography and hierarchical genetic structure are important for determining population structure, and also provide information for developing effective and sustainable management plans. In this study, we assess genetic variation, population structure, and phylogeography of <it>T. mongolica </it>from eight populations. Furthermore, we evaluate the conservation and management units to provide the information for conservation.</p> <p>Results</p> <p>Sequence variation and spatial apportionment of the <it>atp</it>B-<it>rbc</it>L noncoding spacer region of the chloroplast DNA were used to reconstruct the phylogeography of <it>T. mongolica</it>. A total of 880 bp was sequenced from eight extant populations throughout the whole range of its distribution. At the cpDNA locus, high levels of genetic differentiation among populations and low levels of genetic variation within populations were detected, indicating that most seed dispersal was restricted within populations.</p> <p>Conclusions</p> <p>Demographic fluctuations, which led to random losses of genetic polymorphisms from populations, due to frequent flooding of the Yellow River and human disturbance were indicated by the analysis of BEAST skyline plot. Nested clade analysis revealed that restricted gene flow with isolation by distance plus occasional long distance dispersal is the main evolutionary factor affecting the phylogeography and population structure of <it>T</it>. <it>mongolica</it>. For setting a conservation management plan, each population of <it>T</it>. <it>mongolica </it>should be recognized as a conservation unit.</p

Authenticity Preservation with Histogram-Based Reversible Data Hiding and Quadtree Concepts

With the widespread use of identification systems, establishing authenticity with sensors has become an important research issue. Among the schemes for making authenticity verification based on information security possible, reversible data hiding has attracted much attention during the past few years. With its characteristics of reversibility, the scheme is required to fulfill the goals from two aspects. On the one hand, at the encoder, the secret information needs to be embedded into the original image by some algorithms, such that the output image will resemble the input one as much as possible. On the other hand, at the decoder, both the secret information and the original image must be correctly extracted and recovered, and they should be identical to their embedding counterparts. Under the requirement of reversibility, for evaluating the performance of the data hiding algorithm, the output image quality, named imperceptibility, and the number of bits for embedding, called capacity, are the two key factors to access the effectiveness of the algorithm. Besides, the size of side information for making decoding possible should also be evaluated. Here we consider using the characteristics of original images for developing our method with better performance. In this paper, we propose an algorithm that has the ability to provide more capacity than conventional algorithms, with similar output image quality after embedding, and comparable side information produced. Simulation results demonstrate the applicability and better performance of our algorithm

Effects of income and residential area on survival of patients with head and neck cancers following radiotherapy: working age individuals in Taiwan

Objectives The five-year survival rate of head and neck cancer (HNC) after radiotherapy (RT) varies widely from 35% to 89%. Many studies have addressed the effect of socioeconomic status and urban dwelling on the survival of HNC, but a limited number of studies have focused on the survival rate of HNC patients after RT. Materials and methods During the period of 2000–2013, 40,985 working age individuals (20  medium income group > low income group and northern > central > southern > eastern Taiwan. Patients with moderate income levels had a 36.9% higher risk of mortality as compared with patients with high income levels (hazard ratio (HR) = 1.369; p < 0.001). Patients with low income levels had a 51.4% greater risk of mortality than patients with high income levels (HR = 1.514, p < 0.001). Conclusion In Taiwan, income and residential area significantly affected the survival rate of HNC patients receiving RT. The highest income level group had the best survival rate, regardless of the geographic area. The difference in survival between the low and high income groups was still pronounced in more deprived areas

Phosphorous Diffuser Diverged Blue Laser Diode for Indoor Lighting and Communication.

An advanced light-fidelity (Li-Fi) system based on the blue Gallium nitride (GaN) laser diode (LD) with a compact white-light phosphorous diffuser is demonstrated for fusing the indoor white-lighting and visible light communication (VLC). The phosphorous diffuser adhered blue GaN LD broadens luminescent spectrum and diverges beam spot to provide ample functionality including the completeness of Li-Fi feature and the quality of white-lighting. The phosphorous diffuser diverged white-light spot covers a radiant angle up to 120(o) with CIE coordinates of (0.34, 0.37). On the other hand, the degradation on throughput frequency response of the blue LD is mainly attributed to the self-feedback caused by the reflection from the phosphor-air interface. It represents the current state-of-the-art performance on carrying 5.2-Gbit/s orthogonal frequency-division multiplexed 16-quadrature-amplitude modulation (16-QAM OFDM) data with a bit error rate (BER) of 3.1 × 10(-3) over a 60-cm free-space link. This work aims to explore the plausibility of the phosphorous diffuser diverged blue GaN LD for future hybrid white-lighting and VLC systems

Genetic population structure of the alpine species Rhododendron pseudochrysanthum sensu lato (Ericaceae) inferred from chloroplast and nuclear DNA

<p>Abstract</p> <p>Background</p> <p>A complex of incipient species with different degrees of morphological or ecological differentiation provides an ideal model for studying species divergence. We examined the phylogeography and the evolutionary history of the <it>Rhododendron pseudochrysanthum </it>s. l.</p> <p>Results</p> <p>Systematic inconsistency was detected between gene genealogies of the cpDNA and nrDNA. Rooted at <it>R. hyperythrum </it>and <it>R. formosana</it>, both trees lacked reciprocal monophyly for all members of the complex. For <it>R. pseudochrysanthum </it>s.l., the spatial distribution of the cpDNA had a noteworthy pattern showing high genetic differentiation (F_ST= 0.56-0.72) between populations in the Yushan Mountain Range and populations of the other mountain ranges.</p> <p>Conclusion</p> <p>Both incomplete lineage sorting and interspecific hybridization/introgression may have contributed to the lack of monophyly among <it>R. hyperythrum</it>, <it>R. formosana </it>and <it>R. pseudochrysanthum </it>s.l. Independent colonizations, plus low capabilities of seed dispersal in current environments, may have resulted in the genetic differentiation between populations of different mountain ranges. At the population level, the populations of Central, and Sheishan Mountains may have undergone postglacial demographic expansion, while populations of the Yushan Mountain Range are likely to have remained stable ever since the colonization. In contrast, the single population of the Alishan Mountain Range with a fixed cpDNA haplotype may have experienced bottleneck/founder's events.</p

SERPINE2, an inhibitor of plasminogen activators, is highly expressed in the human endometrium during the secretory phase

<p>Abstract</p> <p>Background</p> <p>SERPINE2, also known as protease nexin-1, belongs to the serine protease inhibitor (SERPIN) superfamily. It is one of the potent SERPINs that modulates the activity of plasminogen activators (PAs). PAs and their SERPIN inhibitors, such as SERPINB2 and SERPINE1, were expressed in the human endometrium and were implicated in implantation. However, expression data about SERPINE2 in the human endometrium is still unknown. Thus, we conducted an investigation to reveal the spatiotemporal and cellular expression of SERPINE2 in the human uterus during the menstrual cycle.</p> <p>Methods</p> <p>Seven patients who underwent a hysterectomy and samples of 120 archived patients' endometrial curettage or parts of the uterus that were formalin-fixed and embedded in paraffin. Western blotting was performed to evaluate the specificity and sensitivity of the antibody. Immunohistochemistry was conducted to localize the SERPINE2 expression site. Quantitative analysis was conducted to evaluate expression levels of SERPINE2 in various sub-phases of the menstrual cycle.</p> <p>Results</p> <p>The SERPINE2 protein was primarily detected in the uterine fluid during the mid- and late-secretory phases of the menstrual cycle. It was predominantly expressed in the luminal and glandular epithelium, less in the myometrium, and only dispersedly in certain stromal cells throughout the menstrual cycle. A quantitative analysis of expression levels of SERPINE2 in the glandular epithelium revealed that it was highly expressed in the endometrium during the secretory phase compared to the proliferative phase.</p> <p>Conclusions</p> <p>The SERPINE2 protein is highly expressed in the endometrium during the secretory phase, indicating that it may participate in tissue remodeling involved in implantation.</p

Application of Advanced Framework Technology in Smart Cities to Improve Resource Utilization

Nowadays, the application technology and demand are growth; there have been millions of solutions for user communication in smart cities. However, the quality of the autonomy of handheld devices and the information exchange of applications are functions of requesting services or participating in communications. Therefore, it is very difficult and tedious to implement resource management and control in such an environment. This study here proposes distributed cyber-physical systems (CPS) for agent-based middleware framework (AMF) using to achieve technology, thereby improving the reliability of environmental communication in smart cities. The technical solution has the characteristics of avoiding the problem of data source interruption because of the proxy technology of the linear calculation model. The aforementioned agents are independent and autonomous of each other in terms of providing seamless resource sharing and response scheduling, and have nothing to do with communication time and request queries. In this study, the architecture mainly uses the best linear calculation model to classify overlapping agents, and then allocates non-overlapping resources, and finally analyzes the overall architecture operation performance by responding to processed queries, storage utilization and resource usage, pause time and response

Konsep Demokrasi Politik Dalam Islam

Coexistence of chronic rhinosinusitis (CRS) with asthma appears to impair asthma control. Type-2 innate lymphoid cells (ILC2s) respond to the cytokines of thymic stromal lymphopoietin (TSLP), interleukin (IL)-25 and IL-33, thus contributing to airway diseases such as CRS and asthma. We investigate whether the augmented Th2-cytokines in CRS might be related to sinonasal tract ILC2s corresponding to enhanced IL-25, IL-33 and TSLP release in severe asthmatics, and be involved in asthma control. Twenty-eight asthmatics (12 non-severe and 16 severe) with CRS receiving nasal surgery were enrolled. The predicted FEV1 inversely associated with CRS severity of CT or endoscopy scores. Higher expression of Th2-driven cytokines (IL-4, IL-5, IL-9, and IL-13), TSLP, IL-25 and IL-33 in nasal tissues was observed in severe asthma. Severe asthmatics had higher ILC2 cell counts in their nasal tissues. ILC2 counts were positively correlated with Th2-cytokines. Nasal surgery significantly improved asthma control and lung function decline in severe asthma and CRS. The higher expression of IL-33/ILC2 axis-directed type 2 immune responses in nasal tissue of CRS brought the greater decline of lung function in severe asthma. ILC2-induced the upregulated activity of Th2-related cytokines in asthmatics with CRS may contribute to a recalcitrant status of asthma control