14 research outputs found

    Rezultate partiale privind tipurile de substraturi folosite pentru obţinerea culturilor legumicole de tip microgreens

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    The paper addresses research on the use of four types of substrates (coconut substrate, 80% peat + 20% coconut waste, 80% peat + 20% sand and80% peat + 20% pearlite) to obtain the products microgreens type vegetables, in order to establish the best substrate variant. The best results were obtained on the coconut waste substrate

    Grădini legumicole ornamentale in sistem familial

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    Ornamental vegetable gardens have a long history on the European continent. The design of the gardens is different due to influences originated from customs and traditions, which represent important elements in their composition and are reflected in the vegetable growing methods and species used. The purpose of this paper is to analyse the opportunities that these gardens have and to satisfy the nutritional and aesthetic needs of a family.To reach the aim and proposed objectives a series of experiments and case studies were conducted. By combining the owners underlined nutritional needs from our previous studies and the obtained results from our experiments, applicable solutions were created for family vegetable gardens.From a therapeutic pointof view this type of landscape design helps maintain cultural identity, encouraging communication and socialization between members of a community.The obtained results show that the studied family gardens situated in urban areas have a positive influenceon the sustainability of the community maintaining a “heathy life style” for its inhabitants

    The Circadian Clock Protein Timeless Regulates Phagocytosis of Bacteria in Drosophila

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    Survival of bacterial infection is the result of complex host-pathogen interactions. An often-overlooked aspect of these interactions is the circadian state of the host. Previously, we demonstrated that Drosophila mutants lacking the circadian regulatory proteins Timeless (Tim) and Period (Per) are sensitive to infection by S. pneumoniae. Sensitivity to infection can be mediated either by changes in resistance (control of microbial load) or tolerance (endurance of the pathogenic effects of infection). Here we show that Tim regulates resistance against both S. pneumoniae and S. marcescens. We set out to characterize and identify the underlying mechanism of resistance that is circadian-regulated. Using S. pneumoniae, we found that resistance oscillates daily in adult wild-type flies and that these oscillations are absent in Tim mutants. Drosophila have at least three main resistance mechanisms to kill high levels of bacteria in their hemolymph: melanization, antimicrobial peptides, and phagocytosis. We found that melanization is not circadian-regulated. We further found that basal levels of AMP gene expression exhibit time-of-day oscillations but that these are Tim-independent; moreover, infection-induced AMP gene expression is not circadian-regulated. We then show that phagocytosis is circadian-regulated. Wild-type flies exhibit up-regulated phagocytic activity at night; Tim mutants have normal phagocytic activity during the day but lack this night-time peak. Tim appears to regulate an upstream event in phagocytosis, such as bacterial recognition or activation of phagocytic hemocytes. Interestingly, inhibition of phagocytosis in wild type flies results in survival kinetics similar to Tim mutants after infection with S. pneumoniae. Taken together, these results suggest that loss of circadian oscillation of a specific immune function (phagocytosis) can have significant effects on long-term survival of infection
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