Egy vállalkozás megalapításának kezdeti lépései

A szakdogozat tárgya: hogyan kell megalapítni egy vállalkozást, milyen külső tényezőket kell figyelembe venni és hogyan kell beindítani, kiválasztani a tényezők figyelembe vételével.BscGazdaságinformatiku

Ligand entry in human ileal bile acid-binding protein is mediated by histidine protonation

Abstract Human ileal bile acid-binding protein (hI-BABP) has a key role in the intracellular transport of bile salts. To explore the role of histidine protonation in the binding process, the pH-dependence of bile salt binding and internal dynamics in hI-BABP was investigated using NMR spectroscopy and biophysical tools. Thermodynamic and kinetic measurements show an increase in the overall binding affinity and the association rate constant of the first binding step below the pKa of the histidines, suggesting that ligand binding is favoured by the protonated state. The overlap between residues exhibiting a high sensitivity to pH in their backbone amide chemical shifts and protein regions undergoing a global ms conformational exchange indicate a connection between the two processes. According to 15N NMR relaxation dispersion analysis, the slow motion is most pronounced at and above the pKa of the histidines. In agreement with the NMR measurements, MD simulations show a stabilization of the protein by histidine protonation. Hydrogen-bonding and van der Waals interactions mediating the flow of information between the C/D- and G/H-turn regions hosting the three histidines, suggest a complex way of pH-governed allosteric regulation of ligand entry involving a transition between a closed and a more open protein state

Using contextual knowledge in interactive fault localization

Tool support for automated fault localization in program debugging is limited because state-of-the-art algorithms often fail to provide efficient help to the user. They usually offer a ranked list of suspicious code elements, but the fault is not guaranteed to be found among the highest ranks. In Spectrum-Based Fault Localization (SBFL) – which uses code coverage information of test cases and their execution outcomes to calculate the ranks –, the developer has to investigate several locations before finding the faulty code element. Yet, all the knowledge she a priori has or acquires during this process is not reused by the SBFL tool. There are existing approaches in which the developer interacts with the SBFL algorithm by giving feedback on the elements of the prioritized list. We propose a new approach called iFL which extends interactive approaches by exploiting contextual knowledge of the user about the next item in the ranked list (e. g., a statement), with which larger code entities (e. g., a whole function) can be repositioned in their suspiciousness. We implemented a closely related algorithm proposed by Gong et al. , called Talk . First, we evaluated iFL using simulated users, and compared the results to SBFL and Talk . Next, we introduced two types of imperfections in the simulation: user’s knowledge and confidence levels. On SIR and Defects4J, results showed notable improvements in fault localization efficiency, even with strong user imperfections. We then empirically evaluated the effectiveness of the approach with real users in two sets of experiments: a quantitative evaluation of the successfulness of using iFL , and a qualitative evaluation of practical uses of the approach with experienced developers in think-aloud sessions

Analysis of Aristolochlic Acids and Evaluation of Antibacterial Activity of Aristolochia clematitis L.

Introduction: Several Aristolochia species were used as medicinal herb across Europe and in recent years, their antimicrobial activity has also been investigated. Materials and methods: In this study, A. clematitis was selected to evaluate the aristolochic acids I and II (AA I and AA II) concentrations and the antimicrobial activity of methanol, hexane, butanol, and ethyl acetate extracts of the root, stem, leaf, root, and fruit. AA I and AA II contents were measured by a validated high-performance liquid chromatography–ultraviolet method. Results: Each fraction of the plant contained AA I and AA II and the root was found to have the highest contents of AA I (1.09%) and AA II (0.7454%). The minimum inhibitory concentrations of all extracts were determined by standard microdilution method. The fruit’s extracts showed the most efficient antimicrobial effect against both methicillin sensitive and resistant Staphylococcus aureus strains. Conclusion: Correlation between the AA I and AA II concentrations and the antimicrobial effect was not found