Five principles to navigate a bumpy golden road towards open access

The publishing ecosystem of the future will be built on several models such as offsetting agreements as well as various open access publishing channels. The LIBER Open Access Working Group has issued five principles to support libraries in their efforts to negotiate offsetting deals as they move towards full open access to research information. This article describes why the five principles were created and the underlying considerations and limitations encountered while working on them

Ejemplos del uso del video en los hiperentornos de aprendizaje en el proyecto Galenomedia

El video constituye uno de los medios de enseñanza que más aporta a la calidad delproceso enseñanza-aprendizaje, en particular cuando se emplea en el softwareeducativo. El proyecto nacional para el desarrollo de software educativo en laEnseñanza Médica Superior (EMS), conocido como Galenomedia, que desarrollabásicamente la línea de los Hiperentornos Educativos de Aprendizaje (HEA), hadebatido y analizado en sus talleres y eventos sus ventajas con el fin de potenciarsu empleo. Sin embargo, el análisis de los Hiperentornos producidos en el curso 2010-2011, muestra que aún el video no cumple el rol esperado como medio didáctico en este tipo de software educativo. Este trabajo muestra a través del HEA"Verificación y comprobación de la protección eléctrica en los equipos biomédicos" algunos ejemplos sobre el uso del video en este tipo de software y está dirigidofundamentalmente a los profesores que participan en el proyecto Galenomedia.Palabras clave: software educativo, hiperentornos de aprendizaje, galenomedia,video educativo

Treponema denticola chymotrypsin-like protease as associated with HPV-negative oropharyngeal squamous cell carcinoma

BACKGROUND: An opportunistic oral pathogen, Treponema denticola (Td), has been linked to orodigestive carcinogenesis, but its role in oropharyngeal squamous cell carcinoma (OPSCC) has remained open. We evaluated the presence of Td chymotrypsin-like protease (Td-CTLP) in a series of 201 unselected consecutive OPSCC patients, and the relation of the Td-CTLP to human papillomavirus (HPV) status, to expression of toll-like receptors (TLR) 5, 7, and 9, and to clinical parameters and patient outcome. METHODS: Clinicopathological data came from hospital registries. The expression of cell surface-bound Td-CTLP was evaluated by immunohistochemistry. Immunoexpression of TLRs 5, 7, and 9, and HPV status we studied earlier in this patient series. RESULTS: We detected Td-CTLP in 81% of the OPSCC, and especially in HPV-negative tumours (48% of all OPSCCs). Among the HPV-positive tumours (52% of all OPSCCs), low Td-CTLP expression associated with low TLR 5 and high TLR 7 expression. Among those HPV-negative, higher TLR 5 and lower TLR 7 expression associated with high Td-CTLP expression. Strong Td-CTLP expression associated with poor disease-specific survival, but no similar association among HPV-positive and HPV-negative subgroups emerged. CONCLUSIONS: Td-CTLP was highly expressed in OPSCC and was associated with the HPV status of tumour tissue.Peer reviewe

Clara cell adhesion and migration to extracellular matrix

<p>Abstract</p> <p>Background</p> <p>Clara cells are the epithelial progenitor cell of the small airways, a location known to be important in many lung disorders. Although migration of alveolar type II and bronchiolar ciliated epithelial cells has been examined, the migratory response of Clara cells has received little attention.</p> <p>Methods</p> <p>Using a modification of existing procedures for Clara cell isolation, we examined mouse Clara cells and a mouse Clara-like cell line (C22) for adhesion to and migration toward matrix substrate gradients, to establish the nature and integrin dependence of migration in Clara cells.</p> <p>Results</p> <p>We observed that Clara cells adhere preferentially to fibronectin (Fn) and type I collagen (Col I) similar to previous reports. Migration of Clara cells can be directed by a fixed gradient of matrix substrates (haptotaxis). Migration of the C22 cell line was similar to the Clara cells so integrin dependence of migration was evaluated with this cell line. As determined by competition with an RGD containing-peptide, migration of C22 cells toward Fn and laminin (Lm) 511 (formerly laminin 10) was significantly RGD integrin dependent, but migration toward Col I was RGD integrin independent, suggesting that Clara cells utilize different receptors for these different matrices.</p> <p>Conclusion</p> <p>Thus, Clara cells resemble alveolar type II and bronchiolar ciliated epithelial cells by showing integrin mediated pro-migratory changes to extracellular matrix components that are present in tissues after injury.</p