Investigation of environmental change pattern in Japan

The author has identified the following significant results. A detailed land use classification for a large urban area of Tokyo was made using MSS digital data. It was found that residential, commercial, industrial, and wooded areas and grasslands can be successfully classified. A mesoscale vortex associated with large ocean current, Kuroshio, which is a rare phenomenon, was recognized visually through the analysis of MSS data. It was found that this vortex affects the effluent patterns of rivers. Lava flowing from Sakurajima Volcano was clearly classified for three major erruptions (1779, 1914, and 1946) using MSS data

Deformation of canonical morphisms and the moduli of surfaces of general type

In this article we study the deformation of finite maps and show how to use this deformation theory to construct varieties with given invariants in a projective space. Among other things, we prove a criterion that determines when a finite map can be deformed to a one--to--one map. We use this criterion to construct new simple canonical surfaces with different $c_1^2$ and $\chi$. Our general results enable us to describe some new components of the moduli of surfaces of general type. We also find infinitely many moduli spaces $\mathcal M_{(x',0,y)}$ having one component whose general point corresponds to a canonically embedded surface and another component whose general point corresponds to a surface whose canonical map is a degree 2 morphism.Comment: 32 pages. Final version with some simplifications and clarifications in the exposition. To appear in Invent. Math. (the final publication is available at springerlink.com

Heterozygous mis-sense mutations in Prkcb as a critical determinant of anti-polysaccharide antibody formation

To identify rate-limiting steps in T cell-independent type 2 (TI-2) antibody production against polysaccharide antigens, we performed a genome-wide screen by immunizing several hundred pedigrees of C57BL/6 mice segregating ENU-induced mis-sense mutations. Two independent mutations, Tilcara and Untied, were isolated that semi-dominantly diminished antibody against polysaccharide but not protein antigens. Both mutations resulted from single amino acid substitutions within the kinase domain of Protein Kinase C Beta (PKCβ). In Tilcara, a Ser552>Pro mutation occurred in helix G, in close proximity to a docking site for the inhibitory N-terminal pseudosubstrate domain of the enzyme, resulting in almost complete loss of active, autophosphorylated PKCβI whereas the amount of alternatively spliced PKCβII protein was not markedly reduced. Circulating B cell subsets were normal and acute responses to BCR-stimulation such as CD25 induction and initiation of DNA synthesis were only measurably diminished in Tilcara homozygotes, whereas the fraction of cells that had divided multiple times was decreased to an intermediate degree in heterozygotes. These results, coupled with evidence of numerous mis-sense PRKCB mutations in the human genome, identify Prkcb as a genetically sensitive step likely to contribute substantially to population variability in anti-polysaccharide antibody levels

Earthquake detection capacity of Dense Oceanfloor Network system for Earthquakes and Tsunamis (DONET)

We adopted the Probability-based Magnitude of Completeness (PMC) method and performed a case analysis of the Nankai Trough, a target region monitored for future megathrust earthquakes. JAMSTEC (Japan Agency for Marine-Earth Science and Technology) has created a seismicity catalog that includes events in this region observed by DONET. Using seismicity data for 2015-2019, we found spatiotemporal variability of completeness magnitude Mp. Mp was lower than 1 in one of the areas where stations are densely deployed, whereas Mp was larger than 2 at the periphery and outside of the DONET area. We then evaluated the temporal evolution of Mp, highlighting how the failure of sets of observing stations influenced Mp if not repaired. Stations are aggregated around the 12 science nodes (hubs that connect the stations) and connected through the two oceanfloor backbone cables to JAMSTEC. We explored the possible use of PMC as a tool with simulation computation of node malfunction. A simulation showed that completeness estimates in the area near failure nodes were about 1 magnitude larger. If such failure occurred for nodes near the region which straddles the rupture zones of the previous Tonankai and Nankai earthquakes in 1940's, it would most pronouncedly affect earthquake monitoring among nodes' failures. It is desirable to repair these nodes or replace with new ones when their malfunction occurs. We then demonstrated an example of how to use Mp information as prior knowledge to seismicity-related studies. We used the b value of the Gutenberg-Richter distribution, and computed it taking Mp into consideration. We found that the spatial and temporal changes in b were strongly correlated to the magnitude-6 class slow slip that grew over two years on the Nankai Trough plate boundary, indicating the b value as a proxy that can help to image stress heterogeneity when there is a slow slip event.Comment: 6 figure

4/3-Law of Granular Particles Flowing through a Vertical Pipe

Density waves of granular material (sand) flowing through a vertical pipe have been investigated. Clear density waves emerge when the cock attached to bottom end of the pipe is closed. The FFT power spectra were found to show a stable power-law form $P(f) \sim f^{-\alpha}.$ The value of the exponent was evaluated as $\alpha \cong 4/3$. We also introduce a simple one-dimensional model which reproduces $\alpha = 4/3$ from both simulation and theoretical analysis. (to be published in Phys.Rev.Lett.)Comment: 4 pages, 4 figures, a style fil

First operation and performance of a 200 lt double phase LAr LEM-TPC with a 40x76 cm^2 readout

In this paper we describe the design, construction, and operation of a first large area double-phase liquid argon Large Electron Multiplier Time Projection Chamber (LAr LEM-TPC). The detector has a maximum drift length of 60 cm and the readout consists of a $40\times 76$ cm$^2$ LEM and 2D projective anode to multiply and collect drifting charges. Scintillation light is detected by means of cryogenic PMTs positioned below the cathode. To record both charge and light signals, we have developed a compact acquisition system, which is scalable up to ton-scale detectors with thousands of charge readout channels. The acquisition system, as well as the design and the performance of custom-made charge sensitive preamplifiers, are described. The complete experimental setup has been operated for a first time during a period of four weeks at CERN in the cryostat of the ArDM experiment, which was equipped with liquid and gas argon purification systems. The detector, exposed to cosmic rays, recorded events with a single-channel signal-to-noise ratio in excess of 30 for minimum ionising particles. Cosmic muon tracks and their $\delta$-rays were used to assess the performance of the detector, and to estimate the liquid argon purity and the gain at different amplification fields.Comment: 23 pages, 21 figure

First operation and drift field performance of a large area double phase LAr Electron Multiplier Time Projection Chamber with an immersed Greinacher high-voltage multiplier

We have operated a liquid-argon large-electron-multiplier time-projection chamber (LAr LEM-TPC) with a large active area of 76 $\times$ 40 cm$^2$ and a drift length of 60 cm. This setup represents the largest chamber ever achieved with this novel detector concept. The chamber is equipped with an immersed built-in cryogenic Greinacher multi-stage high-voltage (HV) multiplier, which, when subjected to an external AC HV of $\sim$1 kV$_{\mathrm{pp}}$, statically charges up to a voltage a factor of $\sim$30 higher inside the LAr vessel, creating a uniform drift field of $\sim$0.5 kV/cm over the full drift length. This large LAr LEM-TPC was brought into successful operation in the double-phase (liquid-vapor) operation mode and tested during a period of $\sim$1 month, recording impressive three-dimensional images of very high-quality from cosmic particles traversing or interacting in the sensitive volume. The double phase readout and HV systems achieved stable operation in cryogenic conditions demonstrating their good characteristics, which particularly suit applications for next-generation giant-scale LAr-TPCs.Comment: 26 pages, 19 figure

The clockfront and wavefront model revisited

The currently accepted interpretation of the clock and wavefront model of somitogenesis is that a posteriorly moving molecular gradient sequentially slows the rate of clock oscillations, resulting in a spatial readout of temporal oscillations. However, while molecular components of the clocks and wavefronts have now been identified in the pre-somitic mesoderm (PSM), there is not yet conclusive evidence demonstrating that the observed molecular wavefronts act to slow clock oscillations. Here we present an alternative formulation of the clock and wavefront model in which oscillator coupling, already known to play a key role in oscillator synchronisation, plays a fundamentally important role in the slowing of oscillations along the anterior–posterior (AP) axis. Our model has three parameters which can be determined, in any given species, by the measurement of three quantities: the clock period in the posterior PSM, somite length and the length of the PSM. A travelling wavefront, which slows oscillations along the AP axis, is an emergent feature of the model. Using the model we predict: (a) the distance between moving stripes of gene expression; (b) the number of moving stripes of gene expression and (c) the oscillator period profile along the AP axis. Predictions regarding the stripe data are verified using existing zebrafish data. We simulate a range of experimental perturbations and demonstrate how the model can be used to unambiguously define a reference frame along the AP axis. Comparing data from zebrafish, chick, mouse and snake, we demonstrate that: (a) variation in patterning profiles is accounted for by a single nondimensional parameter; the ratio of coupling strengths; and (b) the period profile along the AP axis is conserved across species. Thus the model is consistent with the idea that, although the genes involved in pattern propagation in the PSM vary, there is a conserved patterning mechanism across species

Circadian Gene Circuitry Predicts Hyperactive Behavior in a Mood Disorder Mouse Model

SummaryBipolar disorder, also known as manic-depressive illness, causes swings in mood and activity levels at irregular intervals. Such changes are difficult to predict, and their molecular basis remains unknown. Here, we use infradian (longer than a day) cyclic activity levels in αCaMKII (Camk2a) mutant mice as a proxy for such mood-associated changes. We report that gene-expression patterns in the hippocampal dentate gyrus could retrospectively predict whether the mice were in a state of high or low locomotor activity (LA). Expression of a subset of circadian genes, as well as levels of cAMP and pCREB, possible upstream regulators of circadian genes, were correlated with LA states, suggesting that the intrinsic molecular circuitry changes concomitant with infradian oscillatory LA. Taken together, these findings shed light onto the molecular basis of how irregular biological rhythms and behavior are controlled by the brain

Exploring rare cellular activity in more than one million cells by a transscale scope

In many phenomena of biological systems, not a majority, but a minority of cells act on the entire multicellular system causing drastic changes in the system properties. To understand the mechanisms underlying such phenomena, it is essential to observe the spatiotemporal dynamics of a huge population of cells at sub-cellular resolution, which is difficult with conventional tools such as microscopy and flow cytometry. Here, we describe an imaging system named AMATERAS that enables optical imaging with an over-one-centimeter field-of-view and a-few-micrometer spatial resolution. This trans-scale-scope has a simple configuration, composed of a low-power lens for machine vision and a hundred-megapixel image sensor. We demonstrated its high cell-throughput, capable of simultaneously observing more than one million cells. We applied it to dynamic imaging of calcium ions in HeLa cells and cyclic-adenosine-monophosphate in Dictyostelium discoideum, and successfully detected less than 0.01% of rare cells and observed multicellular events induced by these cells