Oxygen carriers affect kidney immunogenicity during ex-vivo machine perfusion

Normothermic ex-vivo machine perfusion provides a powerful tool to improve donor kidney preservation and a route for the delivery of pharmacological or gene therapeutic interventions prior to transplantation. However, perfusion at normothermic temperatures requires adequate tissue oxygenation to meet the physiological metabolic demand. For this purpose, the addition of appropriate oxygen carriers (OCs) to the perfusion solution is essential to ensure a sufficient oxygen supply and reduce the risk for tissue injury due to hypoxia. It is crucial that the selected OCs preserve the integrity and low immunogenicity of the graft. In this study, the effect of two OCs on the organ's integrity and immunogenicity was evaluated. Porcine kidneys were perfused ex-vivo for four hours using perfusion solutions supplemented with red blood cells (RBCs) as conventional OC, perfluorocarbon (PFC)-based OC, or Hemarina-M101 (M101), a lugworm hemoglobin-based OC named HEMO2life®, recently approved in Europe (i.e., CE obtained in October 2022). Perfusions with all OCs led to decreased lactate levels. Additionally, none of the OCs negatively affected renal morphology as determined by histological analyses. Remarkably, all OCs improved the perfusion solution by reducing the expression of pro-inflammatory mediators (IL-6, IL-8, TNFα) and adhesion molecules (ICAM-1) on both transcript and protein level, suggesting a beneficial effect of the OCs in maintaining the low immunogenicity of the graft. Thus, PFC-based OCs and M101 may constitute a promising alternative to RBCs during normothermic ex-vivo kidney perfusion

Unexpected large evasion fluxes of carbon dioxide from turbulent streams draining the world’s mountains

Inland waters, including streams and rivers, are active components of the global carbon cycle. Despite the large areal extent of the world’s mountains, the role of mountain streams for global carbon fluxes remains elusive. Using recent insights from gas exchange in turbulent streams, we found that areal CO2 evasion fluxes from mountain streams equal or exceed those reported from tropical and boreal streams, typically regarded as hotspots of aquatic carbon fluxes. At the regional scale of the Swiss Alps, we present evidence that emitted CO2 derives from lithogenic and biogenic sources within the catchment and delivered by the groundwater to the streams. At a global scale, we estimate the CO2 evasion from mountain streams to 167 ± 1.5 Tg C yr−1, which is high given their relatively low areal contribution to the global stream and river networks. Our findings shed new light on mountain streams for global carbon fluxes

Global carbon dioxide efflux from rivers enhanced by high nocturnal emissions

Carbon dioxide (CO2) emissions to the atmosphere from running waters are estimated to be four times greater than the total carbon (C) flux to the oceans. However, these fluxes remain poorly constrained because of substantial spatial and temporal variability in dissolved CO2 concentrations. Using a global compilation of high-frequency CO2 measurements, we demonstrate that nocturnal CO2 emissions are on average 27% (0.9 gC m−2 d−1) greater than those estimated from diurnal concentrations alone. Constraints on light availability due to canopy shading or water colour are the principal controls on observed diel (24 hour) variation, suggesting this nocturnal increase arises from daytime fixation of CO2 by photosynthesis. Because current global estimates of CO2 emissions to the atmosphere from running waters (0.65–1.8 PgC yr−1) rely primarily on discrete measurements of dissolved CO2 obtained during the day, they substantially underestimate the magnitude of this flux. Accounting for night-time CO2 emissions may elevate global estimates from running waters to the atmosphere by 0.20–0.55 PgC yr−1

A Cable Laid Is a Cable Played : On the Hibernation Logic behind Urban Infrastructure Mines

Our societies are reliant on metals to such an extent that the total amounts of some of them in the built environment are comparable in size to the remaining amounts in known mountain ores. Because of concerns about mineral scarcity, the United Nations has assessed alternative sources for metal extraction and targeted urban areas in general and infrastructure systems in particular, since these are large, spatially concentrated and rich in metals. Referring to the possibility of recovering these metal stocks, infrastructure systems constitute what material flow researchers has conceptually termed “urban mines.” While most urban infrastructure is in use, significant amounts of cables and pipes have been disconnected and remain in their subsurface locations; they are “hibernating.” In this article, we analyze the occurrence of such hibernation in the Swedish city of Norrköping's urban infrastructure mine where, we know from a previous study, that every fourth kilo of infrastructure is discarded. Our applied perspective is different from the logic of system expansion as a way to meet increased demand often found in the field of infrastructure studies since we are interested in how systems are disconnected and left behind. This enables us to offer a refined understanding of the concepts of infrastructure “decline” and infrastructure “cold spots.” We argue that to prevent the increase of dormant infrastructures and to engage in the urban mining of already dormant infrastructures, we must develop a sensibility to the materiality of derelict infrastructure components and the underlying causes for why they form different kinds of spatial patterns

Voluntary Associations and Net Fertility During the Swedish Demographic Transition

This study investigates the role of changing social relations for fertility decline during the European fertility transition. The growth of voluntary associations at the end of the nineteenth century entailed a radical shift in the landscape of social relations in Sweden. By combining micro-census data from 1890 to 1900 with local-level membership data for three voluntary association groups, this article assesses the effect of parish-level voluntary association size on net fertility in Sweden using mixed-effects Poisson regression models. The results show that the adoption of fertility limitation during the transition period was associated with the creation and diffusion of the idea of respectability within large social network organisations, an idea that has previously been shown to be connected to fertility limitation. Furthermore, by applying a social network perspective, the results show that the strength of the effect was dependent on the structure of the social networks in terms of size, density, and homogeneity. Voluntary association size had the strongest effect for the free churches, which created dense heterogeneous networks through systems of social control, while the size of the temperance association showed no effect on fertility because the connections between nodes were sparse