Dimensional Issues in Stereolithography

New stereolithography photopolymers have recently been introduced that provide a wider range of functional properties similar to those of high-density polyethylene. One of the important criteria for these materials is the dimensional accuracy and stability in end-use applications as mold masters or the actual functional parts. This work investigates the dimensional stability of one of these new materials with varying amounts of exposure during build. The effect of aging on the part dimensions is reported. The result of environmental humidity extremes at ambient temperature on part dimensions is investigated and compared for parts made from two different families of stereolithography resins, namely DuPont Somos® 7100 and Somos® 8100.Mechanical Engineerin

Remote sensing in the mixing zone

Characteristics of dispersion and diffusion as the mechanisms by which pollutants are transported in natural river courses were studied with the view of providing additional data for the establishment of water quality guidelines and effluent outfall design protocols. Work has been divided into four basic categories which are directed at the basic goal of developing relationships which will permit the estimation of the nature and extent of the mixing zone as a function of those variables which characterize the outfall structure, the effluent, and the river, as well as climatological conditions. The four basic categories of effort are: (1) the development of mathematical models; (2) laboratory studies of physical models; (3) field surveys involving ground and aerial sensing; and (4) correlation between aerial photographic imagery and mixing zone characteristics

Myocardial Relaxation Is Accelerated by Fast Stretch, Not Reduced Afterload

Fast relaxation of cross-bridge generated force in the myocardium facilitates efficient diastolic function. Recently published research studying mechanisms that modulate the relaxation rate has focused on molecular factors. Mechanical factors have received less attention since the 1980s when seminal work established the theory that reducing afterload accelerates the relaxation rate. Clinical trials using afterload reducing drugs, partially based on this theory, have thus far failed to improve outcomes for patients with diastolic dysfunction. Therefore, we reevaluated the protocols that suggest reducing afterload accelerates the relaxation rate and identified that myocardial relengthening was a potential confounding factor. We hypothesized that the speed of myocardial relengthening at end systole (end systolic strain rate), and not afterload, modulates relaxation rate and tested this hypothesis using electrically-stimulated trabeculae from mice, rats, and humans. We used load-clamp techniques to vary afterload and end systolic strain rate independently. Our data show that the rate of relaxation increases monotonically with end systolic strain rate but is not altered by afterload. Computer simulations mimic this behavior and suggest that fast relengthening quickens relaxation by accelerating the detachment of cross-bridges. The relationship between relaxation rate and strain rate is novel and upends the prevailing theory that afterload modifies relaxation. In conclusion, myocardial relaxation is mechanically modified by the rate of stretch at end systole. The rate of myocardial relengthening at end systole may be a new diagnostic indicator or target for treatment of diastolic dysfunction

Yeast through the Ages: a Statistical Analysis of Genetic Changes in Aging Yeast

Microarray technology allows for the expression levels of thousands of genes in a cell to be measured simultaneously. The technology provides great potential in the fields of biology and medicine, as the analysis of data obtained from microarray experiments gives insight into the roles of specific genes and the associated changes across experimental conditions (e.g., aging, mutation, radiation therapy, drug dosage). The application of statistical tools to microarray data can help make sense of the experiment and thereby advance genetic, biological, and medical research. Likewise, microarrays provide an exciting means through which to explore statistical techniques

Analyzing DNA Microarrays with Undergraduate Statisticians

With advances in technology, biologists have been saddled with high dimensional data that need modern statistical methodology for analysis. DNA microarrays are able to simultaneously measure thousands of genes (and the activity of those genes) in a single sample. Biologists use microarrays to trace connections between pathways or to identify all genes that respond to a signal. The statistical tools we usually teach our undergraduates are inadequate for analyzing thousands of measurements on tens of samples. The project materials include readings on microarrays as well as computer lab activities. The topics covered include image analysis, filtering and normalization techniques, and statistical methods. The course materials are designed for someone with little or no statistical background, but due to the novel concepts covered, they could easily be adjusted to accommodate students with practically any background

1942 Ruby Yearbook

A digitized copy of the 1942 Ruby, the Ursinus College yearbook.https://digitalcommons.ursinus.edu/ruby/1044/thumbnail.jp

Evidence of an interaction from resolved stellar populations: The curious case of NGC1313

The galaxy NGC1313 has attracted the attention of various studies due to the peculiar morphology observed in optical bands, although it is classified as a barred, late-type galaxy with no apparent close-by companions. However, the velocity field suggests an interaction with a satellite companion. Using resolved stellar populations, we study different parts of the galaxy to understand further its morphology. Based on HST/ACS images, we estimated star formation histories by means of the synthetic CMD method in different areas in the galaxy. Incompleteness limits our analysis to ages younger than ~100Myr. Stars in the red and blue He burning phases are used to trace the distribution of recent star formation. Star formation histories suggest a burst in the southern-west region. We support the idea that NGC1313 is experiencing an interaction with a satellite companion, observed as a tidally disrupted satellite galaxy in the south-west of NGC1313. However, we do not observe any indication of a perturbation due to the interaction with the satellite galaxy at other locations across the galaxy, suggesting that only a modest-sized companion that did not trigger a global starburst was involved.Comment: 10 pages, 4 figures, accepted for publication in MNRA

Fluorescent Affibody Peptide Penetration in Glioma Margin Is Superior to Full Antibody

Object: Fluorescence imaging has the potential to significantly improve neurosurgical resection of oncologic lesions through improved differentiation between normal and cancerous tissue at the tumor margins. In order to successfully mark glioma tissue a fluorescent tracer must have the ability to penetrate through the blood brain barrier (BBB) and provide delineation in the tumor periphery where heterogeneously intact BBB may exist. In this study it was hypothesized that, due to its smaller size, fluorescently labeled anti-EGFR Affibody protein (~7 kDa) would provide a more clear delineation of the tumor margin than would fluorescently labeled cetuximab, a full antibody (~150 kDa) to the epidermal growth factor receptor (EGFR). Methods: Cetuximab and anti-EGFR targeted Affibody were conjugated to two different fluorescent dyes (both emitting in the near-infrared) and injected intravenously into 6 athymic mice which were inoculated orthotopically with green fluorescent protein (GFP) expressing human U251 glioma cells. Each mouse was sacrificed at 1-h post injection, at which time brains were removed, snap frozen, sectioned and quantitatively analyzed for fluorescence distribution. Results: Ex vivo analysis showed on average, nearly equal concentrations of cetuximab and Affibody within the tumor (on average Affibody made up 49 ± 6% of injected protein), however, the cetuximab was more confined to the center of the tumor with Affibody showing significantly higher concentrations at the tumor periphery (on average Affibody made up 72 ± 15% of injected protein in the outer 50 um of the tumor). Further ex vivo analysis of detection studies showed that the Affibody provided superior discrimination for differentiation of tumor from surrounding normal brain. Conclusions: The present study indicates that fluorescently labeled anti-EGFR Affibody can provide significantly better delineation of tumor margins than a fluorescently labeled anti-EGFR antibody and shows considerable potential for guiding margin detection during neurosurgery

Quantitative Imaging of Scattering Changes Associated with Epithelial Proliferation, Necrosis and Fibrosis in Tumors Using Microsampling Reflectance Spectroscopy

Highly localized reflectance measurements can be used to directly quantify scatter changes in tissues. We present a microsampling approach that is used to raster scan tumors to extract parameters believed to be related to the tissue ultrastructure. A confocal reflectance imager was developed to examine scatter changes across pathologically distinct regions within tumor tissues. Tissue sections from two murine tumors, AsPC-1 pancreas tumor and the Mat-LyLu Dunning prostate tumor, were imaged. After imaging, histopathology-guided region-of-interest studies of the images allowed analysis of the variations in scattering resulting from differences in tissue ultra-structure. On average, the median scatter power of tumor cells with high proliferation index (HPI) was about 26% less compared to tumor cells with low proliferation index (LPI). Necrosis exhibited the lowest scatter power signature across all the tissue types considered, with about 55% lower median scatter power than LPI tumor cells. Additionally, the level and maturity of the tumor\u27s fibroplastic response was found to influence the scatter signal. This approach to scatter visualization of tissue ultrastructure in situ could provide a unique tool for guiding surgical resection, but this kind of interpretation into what the signal means relative to the pathology is required before proceeding to clinical studies