Molecular cloning and characterization of the porcine prostaglandin transporter (SLCO2A1): evaluation of its role in F4 mediated neonatal diarrhoea

<p>Abstract</p> <p>Background</p> <p>Because prostaglandins are involved in many (patho)physiological processes, <it>SLCO2A1 </it>was already characterized in several species in an attempt to unravel specific processes/deficiencies. Here, we describe the molecular cloning and characterization of the porcine ortholog in order to evaluate its possible involvement in F4 enterotoxigenic <it>E. coli </it>mediated neonatal diarrhoea, based on a positional candidate gene approach study.</p> <p>Results</p> <p>Porcine <it>SLCO2A1 </it>is organized in 14 exons, containing an open reading frame of 1935 bp, encoding a 12-transmembrane organic anion cell surface transporter of 644 aa. The -388 to -5 upstream region comprises a (CpG)₄₈island containing a number of conserved promoter elements, including a TATA box. A potential alternative promoter region was found in the conserved -973 to -700 upstream region. No consensus polyadenylation signal was discovered in the 3' UTR. Repeat sequences were found in 15% of all the non coding sequences.</p> <p>As expected for a multifunctional protein, a wide tissue distribution was observed. mRNA expression was found in the adrenal gland, bladder, caecum, colon (centripetal coil/centrifugal coil), diaphragm, duodenum, gallbladder, heart, ileum, jejunum, kidney, liver, longissimus dorsi muscle, lung, lymph node, mesenterium, rectum, spleen, stomach, tongue and ureter, but not in the aorta, oesophagus and pancreas.</p> <p>The promoter region and the exons (including the splice sites) of <it>SLCO2A1 </it>were resequenced in 5 F4ab/ac receptor positive and 5 F4ab/ac receptor negative pigs. Two silent and 2 missense (both S → L at position 360 and 633) mutations were found, but none was associated with the F4ab/ac receptor phenotype. In addition, no phenotype associated differential mRNA expression or alternative/abberant splicing/polyadenylation was found in the jejunum.</p> <p>Conclusion</p> <p>The molecular cloning and characterization of porcine <it>SLCO2A1 </it>not only contributes to the already existing knowledge about the transporter in general, but enables studies on porcine prostaglandin related processes/deficiencies as patient and/or model. Here we examined its possible involvement as receptor in F4 enterotoxigenic <it>E. coli </it>mediated neonatal diarrhoea. Because no phenotype associated differences could be found in the gene sequence nor in its jejunal transcription profile of F4ab/ac receptor positive/negative pigs, SLCO2A1 can most likely be excluded as receptor for F4 bacteria.</p

Dans quelle mesure le commerce d'organes est-il acceptable ?des principes a la realite

Organ trade is unlawful ;organ gift is promoted on condition it is gratiutous, anonymous and spontaneous. The idea of graft and transplantation is understood differently according to culture, religion and ability of a region to supply to its needs in the field of health. The patient's eagerness can explain his quest of a donor in whatever country he is. Even those physicians who have strict ethical guidelines must pay attention not to be involved in blamable jobs. The only way to avoid rewarded organ gifts is to prohibit transplantation touring and grafting from friends, at the risk to loose true-hearted and noble donors.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

De la paix armée à la crise permanente. Les fêlures du modèle belge de concertation

status: publishe

Subcutaneous parathyroid autografts (multiple letters)

SCOPUS: le.jinfo:eu-repo/semantics/publishe

Cardiovascular effects of metacrylate methyl monomer intravenous injections in dogs

SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Cardiovascular effects of methyl methacrylate monomer

SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Madura's foot in a renal transplant patient: Report of a case

A 40-year-old kidney transplant male recipient was hospitalized for chronic abscess of the right foot in a context of immunodepression. The patient came from Djibouti and was in Belgium for a few days. He presented a right foot with a swelling localized on the first metatarsophalangeal joint which was excoriated (Figures 1 and 2) and was self-treated ineffectively with various local antiseptics for several months. He was in the operating room for an open biopsy done by plantar and dorsal approach to confirm the fungal infection. Treatment was not started with oral itraconazole because of the good evolution of the lesion. Pain diminished after a few days, and the patient was able to walk after a few weeks. © The Author 2011.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Surgical anatomy of hyperparathyroidism.

The number of glands and the exact location of each normal and pathological parathyroid identified in 416 parathyroidectomies for primary and secondary hyperparathyroidism have been recorded during the last 25 years (1966-1990). Nineteen per cent of all glands were found in ectopic locations. Half of these were distant from the thyroid lobes, most frequently posteriorly alongside the oesophagus, or in the upper anterior mediastinum encapsulated in the thymic remnant. Their discovery required, in some cases, extensive dissections, but a sternotomy was necessary in two cases only. Parathyroid glands may be concealed in the thyroid or in other aberrant locations, more rarely encountered. Supernumerary parathyroids were observed in 5% of cases. They were often pathological and were often in abnormal positions. A sound knowledge of the anatomic variations and of the paths of embryologic migration of the parathyroids are the essential clues for their detection.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Kidney transplantation with ureteral implantation in a neurogenic bladder

SCOPUS: cp.jinfo:eu-repo/semantics/publishe

CONDYLOME ACUMINE GEANT DE L'ANUS: TUMEUR DE BUSCHKE-LOEWENSTEIN

The authors report a case of a Buschke-Loewenstein tumor located at the anus. Initial treatment with Bleomycin was remarkably effective. A recurrence 3 months later did not respond any more to Bleomycin. Multiple surgical resections were performed in association with this form of chemotherapy.SCOPUS: NotDefined.jinfo:eu-repo/semantics/publishe