Thermal performance of scleractinian corals along a latitudinal gradient on the Great Barrier Reef

Species have evolved different mechanisms to cope with spatial and temporal temperature variability. Species with broad geographical distributions may be thermal generalists that perform well across a broad range of temperatures, or they might contain subpopulations of locally adapted thermal specialists. We quantified the variation in thermal performance of two coral species, Porites cylindrica and Acropora spp., along a latitudinal gradient over which temperature varies by approximately 68C. Photosynthesis rates, respiration rates, maximum quantum yield and maximum electron transport rates were measured on coral fragments exposed to an acute temperature increase and decrease up to 58C above and below the local average temperature. Results showed geographical variation in the performance curves of both species at holobiont and symbiont level, but this did not lead to an alignment of the optimal temperature for performance with the average temperature of the local environment, suggesting suboptimal coral performance of these coral populations in summer. Furthermore, symbiont thermal performance generally had an optimum closer to the average environmental temperature than holobiont performance, suggesting that symbionts have a higher capacity for acclimatization than the coral host, and can aid the coral host when temperatures are unfavourable. This article is part of the theme issue 'Physiological diversity, biodiversity patterns and global climate change: testing key hypotheses involving temperature and oxygen'

Micellar dye shuttle between water and an ionic liquid

The reversible transfer of poly(2-ethyl-2-oxazoline)-block-poly(2-nonyl-2-oxazoline) nanocarriers comprising encapsulated dyes is demonstrated between water and an ionic liquid. This dye transfer concept is shown to be applicable for loading and delivery of dye molecules as well as to provide a protective environment for the encapsulated dye

The indirect effects of ocean acidification on corals and coral communities

Ocean acidification (OA) is a major threat to marine calcifying organisms. This manuscript gives an overview of the physiological effects of acidification on reef-building corals from a cellular to population scale. In addition, we present the first review of the indirect effects resulting from altered species interactions. We find that the direct effects of acidification are more consistently negative at larger spatial scales, suggesting an accumulation of sub-lethal physiological effects can result in notable changes at a population and an ecosystem level. We identify that the indirect effects of acidification also have the potential to contribute to declines in coral cover under future acidified conditions. Of particular concern for reef persistence are declines in the abundance of crustose coralline algae which can result in loss of stable substrate and settlement cues for corals, potentially compounding the direct negative effects on coral recruitment rates. In addition, an increase in the abundance of bioeroders and bioerosive capacity may compound declines in calcification and result in a shift towards net dissolution. There are significant knowledge gaps around many indirect effects, including changes in herbivory and associated coral–macroalgal interactions, and changes in habitat provision of corals to fish, invertebrates and plankton, and the impact of changes to these interactions for both individual corals and reef biodiversity as structural complexity declines. This research highlights the potential of indirect effects to contribute to alterations in reef ecosystem functions and processes. Such knowledge will be critical for scaling-up the impacts of OA from individual corals to reef ecosystems and for understanding the effects of OA on reef-dependent human societies

Temperature induced solubility transitions of various poly(2-oxazoline)s in ethanol-water solvent mixtures

The solution behavior of a series of poly(2-oxazoline)s with different side chains, namely methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, pentyl, hexyl, heptyl, octyl, nonyl, phenyl and benzyl, are reported in ethanol-water solvent mixtures based on turbidimetry investigations. The LCST transitions of poly(2-oxazoline) s with propyl side chains and the UCST transitions of the poly(2-oxazoline) s with more hydrophobic side chains are discussed in relation to the ethanol-water solvent composition and structure. The poly(2-alkyl-2-oxazoline) s with side chains longer than propyl only dissolved during the first heating run, which is discussed and correlated to the melting transition of the polymers

Poly(2-cyclopropyl-2-oxazoline): from rate acceleration by Cyclopropyl to Thermoresponsive properties

The synthesis and microwave-assisted living cationic ring-opening polymerization of 2-cyclopropyl-2-oxazoline is reported revealing the fastest polymerization for an aliphatic substituted 2-oxazoline to date, which is ascribed to the electron withdrawing effect of the cyclopropyl group. The poly(2-cyclopropyl-2-oxazoline) (pCPropOx) represents an alternative thermo-responsive poly(2-oxazoline) with a reversible critical temperature close to body temperature. The cloud point (CP) of the obtained pCPropOx in aqueous solution was evaluated in detail by turbidimetry, dynamic light scattering (DLS) and viscosity measurements. pCPropOx is amorphous with a significantly higher glass transition temperature (T(g) similar to 80 degrees C) compared to the amorphous poly(2-n-propyl-2-oxazoline) (pnPropOx) (T(g) similar to 40 degrees C), while poly(2-isopropyl-2-oxazoline) piPropOx is semicrystalline. In addition, a pCPropOx comb polymer was prepared by methacrylic acid end-capping of the living cationic species followed by RAFT polymerization of the macromonomer. The polymer architecture does not influence the concentration dependence of the CP, however, both the CP and T(g) of the comb polymer are lower due to the increased number of hydrophobic end groups

Acoustic Immunosensing of Exosomes Using a Quartz Crystal Microbalance with Dissipation Monitoring

Exosomes are endocytic lipid-membrane bound bodies with the potential to be used as biomarkers in cancer and neurodegenerative disease. The limitations and scarcity of current exosome characterization approaches have led to a growing demand for translational techniques, capable of determining their molecular composition and physical properties in physiological fluids. Here, we investigate label-free immunosensing, using a quartz crystal microbalance with dissipation monitoring (QCM-D), to detect exosomes by exploiting their surface protein profile. Exosomes expressing the transmembrane protein CD63 were isolated by size-exclusion chromatography from cell culture media. QCM-D sensors functionalized with anti-CD63 antibodies formed a direct immunoassay toward CD63-positive exosomes in 75% v/v serum, exhibiting a limit-of-detection of 2.9 × 108 and 1.4 × 108 exosome sized particles (ESPs)/mL for frequency and dissipation response, respectively, i.e., clinically relevant concentrations. Our proof-of-concept findings support the adoption of dual-mode acoustic analysis of exosomes, leveraging both frequency and dissipation monitoring for use in bioanalytical characterization

Editorial

‘A new spring, and a new sound’, so begins a famous Dutch poem. Will the birds sing differently, as the poet wishes in the next line? BKI, now 178 years old—surely one of the longest-running journals of Southeast Asian studies in the world, and known around its original home, the KITLV (Royal Institute), as ‘the Old Dame’—starts 2022 with a remarkable new development. For this reason, the first issue of the year opens with an Editorial. After generations of sound and steady editing under the direction of two scholars, one in the role of Chief Editor and one as Managing Editor, from now on, BKI will be run by an expanded editorial team of seven scholars drawn both from the geographical region, Southeast Asia, and the disciplines in the humanities and social sciences that BKI is dedicated to examining..

Synthesis of novel boronic acid-decorated poly(2-oxazoline)s showing triple-stimuli responsive behavior

Boronic acid-functionalized (co)polymers have gained increasing attention in the field of responsive polymers and polymeric materials due to their unique characteristics and responsiveness towards both changes in pH and sugar concentrations. This makes these (co)polymers excellently suited for various applications including responsive membranes, drug delivery applications and sensor materials. Unfortunately, boronic acid-based polymer research is also notorious for its challenging monomer synthesis and polymerization and its overall difficult polymer purification and manipulation. In light of this, many research groups have focused their attention on the optimization of various polymerization techniques in order to expand the field of BA-research including previously unexplored monomers and polymerization techniques. In this paper, a new post-polymerization modification methodology was developed allowing for the synthesis of novel boronic acid-decorated poly(2-alkyl-2-oxazoline) (PAOx) copolymers, utilizing the recently published PAOx methyl ester reaction platform. The developed synthetic pathway provides a straightforward method for the introduction of pH- and glucose-responsiveness, adding this to the already wide variety of possible responsive PAOx-based systems. The synthesized BA-decorated PAOx are based on the thermoresponsive poly(2-n-propyl-2-oxazoline) (PnPropOx). This introduces a pH and glucose dependence on both cloud and clearance point temperatures of the copolymer in aqueous and pH-buffered conditions, yielding a triply-responsive (co)polymer that highlights the wide variety of obtainable properties using this pathway

Responsive glyco-poly(2-oxazoline)s: synthesis, cloud point tuning, and lectin binding

A new sugar-substituted 2-oxazoline monomer was prepared using the copper-catalyzed alkyne-azide cycloaddition (CuAAC) reaction. Its copolymerization with 2-ethyl-2-oxazoline as well as 2-(dec-9-enyl)-2-oxazoline, yielding well-defined copolymers with the possibility to tune the properties by thiol-ene "click" reactions, is described. Extensive solubility studies on the corresponding glycocopolymers demonstrated that the lower critical solution temperature behavior and pH-responsiveness of these copolymers can be adjusted in water and phosphate-buffered saline (PBS) depending on the choice of the thiol. By conjugation of 2,3,4,6-tetra-O-acetyl-1-thio-beta-D-glucopyranose and subsequent deprotection of the sugar moieties, the hydrophilicity of the copolymer could be increased significantly, allowing a cloud-point tuning in the physiological range. Furthermore, the binding capability of the glycosylated copoly(2-oxazoline) to concanavalin A was investigated