Perpustakaan Umum Malang Dengan Kombinasi Taman Vertikal Dan Ventilasi Untuk Perancangan Ruang Baca

Kualitas udara dalam ruang merupakan sebuah interaksi yang dapat berubah baiksecara konstan mau pun tidak yang diakibatkan oleh beberapa faktor yangmempengaruhi baik dari lingkungan luar mau pun lingkungan dalam. Salah saturuangan yang berpotensi tinggi untuk mengalami masalah polusi udara dalam ruangadalah ruang perpustakaan. Hal ini disebabkan oleh kondisi lingkungan eksternalseperti debu yang terbawa angin dan kondisi internal yaitu bakteri yang terbawa padabuku-buku lama yang dihirup oleh pelaku aktifitas perpustakaan. Dari faktor eksternal,salah satu penyebabnya ialah debu, tanah, dan polutan yang terbawa di udara masuk kedalam ruang perpustakaan. Pengoperasian sistem ventilasi bangunan berperan pentingdalam membawa udara masuk ke dalam ruangan. Salah satu strategi yang telahdisebutkan ialah penggunaan filter. Filter pada ventilasi berfungsi sebagai penyerappolusi yang terbawa angin luar masuk ke ruang dalam. Terdapat beberapa cara untukfiltrasi pada bangunan salah satunya adalah taman vertikal. Diharapkan penggunaankombinasi taman vertikal dan ventilasi dapat menjadi sumber penghawaan alami yangtetap memperhatikan kualitas udara dalam pada perpustakaan agar masalah buruknyakualitas udara ruang dalam pada perpustakaan dapat direduksi

Additional file 1: of Informative gene selection and the direct classification of tumors based on relative simplicity

The binary-discriminative informative genes selected by RS method of nine datasets. (XLS 26 kb

Picroside II protects the blood-brain barrier by inhibiting the oxidative signaling pathway in cerebral ischemia-reperfusion injury

<div><p>Background and purpose</p><p>Thrombolysis is used to improve cerebral circulation; at the same time, neuroprotective drugs such as antioxidants should also be used. The aim of these experiments was to explore the protective mechanism of an antioxidant, picroside II, on the blood-brain barrier (BBB) after cerebral ischemia-reperfusion (CI/R) injury.</p><p>Methods</p><p>To observe the antagonistic effect of picroside II on CI/R damage, the neurological deficit score and the infarct volume were measured. To detect the protective effect of picroside II on nerve cells and the BBB, the morphology and structure of cortical brain tissue were observed, respectively. To investigate the antioxidant effect and mechanism of picroside II, reactive oxygen species (ROS) content, the activity of Nicotinamide adenine dinucleotide phosphate oxidase (NADPH oxidase), and the protein levels of Nox2 and Rac-1 were detected. To investigate the protective mechanism of picroside II on the BBB, the levels of ROCK, MLCK, MMP-2 and claudin-5 were tested.</p><p>Results</p><p>A higher neurological score, bigger cortex infarction, more damaged neuron structure and injured BBB, increased content of ROS and activity of NADPH oxidase, higher protein levels of Nox2, Rac-1, ROCK, MLCK and MMP-2 and lower levels of claudin-5 were observed in the model group. In the picroside group, the neurological score, neuronal damage, BBB injury, ROS content and NADPH oxidase activity were reduced (<i>P</i><0.05), and the protein levels of Rac-1, Nox2, ROCK, MLCK and MMP-2 were down-regulated (<i>P</i><0.05), while the expression of claudin-5 was up-regulated (<i>P</i><0.05).</p><p>Conclusions</p><p>Picroside II could protect the nervous system possibly through reducing the content of ROS by down-regulating the expression of Rac-1 and Nox2 and could protect the BBB through reducing the expression of ROCK, MLCK, and MMP-2, while enhancing the expression of claudin-5.</p></div

The protein expression level of Rac-1 and Nox2.

<p>A. Sham group; B. Model group; C. Treatment group; D. Positive control group; E. Treatment + Positive control group; F. Agonist group; G. Agonist + Treatment group; H. Vehicle group. **<i>P</i><0.01 compared to group A; *<i>P</i><0.05 compared to group A; ##<i>P</i><0.01 compared to group B; #<i>P</i><0.05 compared to group B; △<i>P</i><0.05 compared to group F. The data were compared with one-way ANOVA; Values are presented as means ± SD; n = 5.</p

Rapid and Sensitive Detection of Cancer Cells Based on the Photothermal Effect of Graphene Functionalized Magnetic Microbeads

A strategy based on an immune-graphene oxide (GO)-magnetic microbead complex for the sensitive, rapid, portable, and low-cost detection of cancer cells was developed. The high-efficiency cell capture and high sensitive thermal contrast detection could be simultaneously achieved using magnetic microbeads and the photothermal effect of GOs. The temperature variation caused by irradiating the GOs with a laser was used to establish the standard curve of temperature variation and cancer cell number. Under optimal conditions, the limit of detection could reach 100 cells. 4T1 cells spiked in human blood could be successfully detected in 1.5 h, and the recovery was between 90.8% and 116.5%

miR-410 targets at MDM2 in GC cells.

<p>(A) The sequences of miR-410 binding sites within the human MDM2 3′UTRs and schematic reporter constructs, in this panel, c-MDM2-WT represent the reporter constructs containing the entire 3′UTR sequences of MDM2. C-MDM2-MUT represent the reporter constructs containing mutated nucleotides. (B) The analysis of the relative luciferase activities of MDM2-WT, MDM2-MUT in 293T cells. The error bars are derived from triplicate expriments. (C) qRT-PCR analysis of MDM2 mRNA expression in HGC-27 cells after treatment with miRNA mimics or scramble or no transfection. The expression of MDM2 was normalized to GAPDH. (D) Western blot analysis of MDM2 expression in HGC-27 cells transfected with miR-410 mimics or scramble or no transfection. GAPDH was also detected as a loading control.</p

MicroRNA-410 Suppresses Migration and Invasion by Targeting MDM2 in Gastric Cancer

<div><p>Gastric cancer is one of the most frequent malignancies in tumors in the East Asian countries. Identifying precise prognostic markers and effective therapeutic targets is important in the treatment of gastric cancer. microRNAs (miRNAs) play important roles in tumorigenesis. However, the mechanisms by which miRNAs regulate gastric cancer metastasis remain poorly understood. In this study, we found that the levels of miR-410 in gastric cancer and cell lines were much lower than that in the normal control, respectively, and the lower level of miR-410 was significantly associated with lymph-node metastasis. Transfection of miR-410 mimics could significantly inhibit the cell proliferation, migration and invasion in the HGC-27 gastric cancer cell lines. In contrast, knockdown of miR-410 had the opposite effect on the cell proliferation, migration and invasion. Moreover, we also found that MDM2 was negatively regulated by miR-410 at the post-transcriptional level, via a specific target site with the 3′UTR by luciferase reporter assay. The expression of MDM2 was inversely correlated with miR-410 expression in gastric cancer tissues, and overexpression of MDM2 in miR-410-transfected gastric cancer cells effectively rescued the inhibition of cell proliferation and invasion caused by miR-410. Thus, our findings suggested that miR-410 acted as a new tumor suppressor by targeting the MDM2 gene and inhibiting gastric cancer cells proliferation, migration and invasion. The findings of this study contributed to the current understanding of these functions of miR-410 in gastric cancer.</p></div

Deep-Red and Near-Infrared Xanthene Dyes for Rapid Live Cell Imaging

In this work, two xanthene dyes (<b>H-hNR</b> and <b>TF-hNR</b>) have been synthesized by a convenient and efficient method. These two dyes exhibited deep-red and near-infrared emissions, high fluorescence quantum yields, and good photostability. Their structure–optical properties were investigated by X-ray crystal structure analysis and density functional theory calculations. Live cell imaging data revealed that <b>H-hNR</b> and <b>TF-hNR</b> could rapidly stain both A549 and HeLa cells with low concentrations. The excellent photophysical and imaging properties render them as promising candidates for use in live cell imaging

The morphology and structure of neurons in the cortex of rats, HE ×400.

<p>A. Sham group; B. Model group; C. Treatment (picroside II) group; D. Positive control (apocynin) group; E. Treatment + Positive control (picroside II + apocynin) group; F. Agonist (TBCA) group; G. Agonist + Treatment (TBCA + picroside II) group; H. Vehicle (DMSO) group. ***<i>P</i><0.001 compared to group A; ## <i>P</i><0.01 compared to group B; ### <i>P</i><0.001 compared to group B; △△<i>P</i><0.01 compared to group F. The data were compared with one-way ANOVA; Values are presented as means ± SD; n = 5.</p

Overexpression of miR-410 inhibited the cell proliferation, migration, and invasion in gastric cancer cells (A) Expression levels of miR-410 were examined by qRT-PCR after transfection of 20 nmol/L of miR-410 mimics, inhibitors or sramble or no transfection.

<p>The expression of miR-410 was normalized to U6 snRNA. (B) The cells treated with miR-410 mimics, inhibitors or sramble or no transfection were measured by CCK8 assay at different time periods. (C) Transwell analysis of HGC-27 cells after treatment with miRNA mimics, inhibitors or scramble or no transfection; the relative ratio of migrated cells per field is shown below. (D) Transwell analysis of HGC-27 cells after treatment with miRNA mimics, inhibitors or scramble or no transfection; the relative ratio of invasive cells per field is shown below, *p<0.05, ** p<0.01, and ***p<0.001.</p