100 research outputs found
To P or not to P, is that the question? Rethinking experimental design and data analysis to improve biological significance beyond the statistical significance
The use of remote microphone systems in unilateral hearing loss: a preliminary study among Brazilian children and teenagers
Due to the large number of individuals with Unilateral Hearing Loss (UHL) and the recommendation to use hearing assistive devices, studies are required to define possibilities of intervention for this population. Objective: To evaluate the performance of the Remote Microphone System (RMS) in children with UHL. Methodology: Prospective clinical study with a convenience sample. Eleven children (mean age of 9.2 years) with severe and profound sensorineural UHL, hearing aid users and enrolled in regular schools participated in the study. They were evaluated using the Hearing in Noise Test (HINT), the Classroom Participation Questionnaire (CPQ), and the Sustained Auditory Attention Ability Test (SAAAT) with RMS. Results: HINT results were analyzed using variance to three criteria of repeated measures, which revealed differences between intervention, position, and time factors and significant interaction between these three factors. The comparative analysis of the results from CPQ showed significant differences in the statistical t-test (p=<0.001) for all subscales. The analysis of variance at two repeated measures criteria used in the study of SAAAT revealed a difference between intervention and time, and both interacted significantly. Conclusion: The RMS associated with a hearing aid was effective for individuals with UHL
Desgaste e rugosidade superficial de cimentos de ionômero de vidro utilizados como selantes, após escovação simulada
O presente estudo foi conduzido in vitro com o intuito de constatar as propriedades (desgaste e rugosidade) dos CIV, as quais influenciam na sua indicação como material selador de fossas e fissuras. Os materiais empregados foram Fuji Plus, Ketac-Molar e Vitremer (duas proporções: 1:1 e ¼:1). O selante Delton foi controle. A determinação do desgaste foi obtida através da quantidade de massa perdida após a escovação e a rugosidade através da análise quantitativa da superfÃcie. Os resultados mostraram que o Vitremer diluÃdo e o Fuji Plus apresentaram maior grau de desgaste e maior aumento de rugosidade. Apesar de clinicamente se encontrar um maior uso dos ionômeros de vidro cimentantes ou diluÃdos como forma alternativa para material selador; este trabalho permitiu concluir que estes possuem propriedades bastante inferiores quando comparados aos ionômeros restauradores que, por sua vez, possuem resultados semelhantes ao selante resinoso.The purpose of this study was to evaluate, in vitro, the properties (wear and roughness) of glass ionomer cements that could influence their indication as pit and fissure sealants. The utilized materials were Fuji Plus, Ketac-Molar and Vitremer (in two different proportions: 1:1 and ¼:1). The resin-based sealant Delton was used as control. By means of an electronic balance (precision of 10-4 g), wear was measured in function of weight loss after simulated toothbrushing. Superficial roughness was determined by means of a surface roughness-measuring apparatus. The results revealed that diluted Vitremer and Fuji Plus were less resistant to toothbrushing abrasion and had the greatest increase in superficial roughness.Although in clinical situations luting or diluted ionomer cements are often utilized as alternatives to resin-based sealants, the resultsof this study revealed that the properties of those cements are worse than those of restorative ionomers, whichpresented results similar to those of the evaluated resin sealant
Efeito de diferentes métodos de profilaxia sobre o esmalte hÃgido e desmineralizado
Considering the importance of professional plaque control for caries prevention, this study comprised an in vitro evaluation of wear by two prophylaxis methods (sodium bicarbonate jet - Profident and pumice and brush) on sound bovine enamel and with artificial carious lesions. Sixty enamel fragments were employed (4x4mm), which were divided into 4 groups: GI - 15 sound blocks treated with pumice and brush; GII - 15 sound blocks treated with Profident; GIII - 15 demineralized blocks treated with pumice and brush, and GIV - 15 demineralized blocks treated with Profident. In the fragments of Groups III and IV, artificial carious lesions were simulated by immersion in 0.05M acetic acid solution 50% saturated with bovine enamel powder at 37ºC for 16h. The specimens were submitted to the prophylactic treatments for 10 seconds. Wear analysis was performed by profilometer and revealed the following results: 0.91µm - GI; 0.42µm - GII; 1.6µm - GIII, and 0.94µm - GIV. The two-way ANOVA and Tukey's test (pTendo em vista a importância do controle profissional da placa na prevenção da cárie, este estudo avaliou in vitro o desgaste de dois métodos de profilaxia (jato de bicarbonato de sódio-Profident e escova de Robinson com pasta de pedra pomes) sobre o esmalte bovino hÃgido e com lesões artificiais de cárie. Foram utilizados 60 fragmentos de esmalte (4 X 4mm), divididos em 4 grupos: GI- 15 blocos hÃgidos tratados com escova de Robinson e pasta de pedra pomes; GII- 15 blocos hÃgidos tratados com Profident; GIII- 15 blocos desmineralizados tratados com escova de Robinson e pedra pomes e GIV- 15 blocos desmineralizados tratados com Profident. Nos fragmentos dos grupos III e IV foram simuladas lesões artificiais de cárie através da imersão em solução de ácido acético 0,05M, 50% saturada com pó de esmalte bovino, a 37ºC por 16 h. Os espécimes foram submetidos aos tratamentos profiláticos durante 10 segundos. A análise do desgaste foi feita por meio de perfilometria, encontrando-se os seguintes resultados: 0,91µm-GI; 0,42µm-GII; 1,6µm-GIII e 0,94µm-GIV. Através do teste ANOVA a dois critérios e do teste de Tukey (
The effects of frenotomy on breastfeeding
Although the interference of tongue-tie with breastfeeding is a controversial subject, The use of lingual frenotomy has been widely indicated by health professionals. Objective : To observe changes in breastfeeding patterns after lingual frenotomy concerning the number of sucks, pause length between groups of sucking and mother's complaints. Material and Methods : Oral yes/no questions about breastfeeding symptoms and sucking/swallowing/breathing coordination were answered by the mothers of 109, 30 day old infants. On the same day the infants had their lingual frenulum assessed by administering a lingual frenulum protocol. After the assessment, all tongue-tied infants were referred for frenotomy; nevertheless, only 14 underwent the surgery. Of the 109 infants, 14 infants who did not have frenulum alterations were included as controls. Birth order and gender were the criteria for recruiting the control group. The tongue-tied infants underwent lingual frenotomy at 45 days of age. At the conclusion of the frenotomy, the infants were breastfed. At 75 days old, both groups – control and post-frenotomy – were reassessed. Before the reassessment the same oral yes/no questions were answered by the mothers of the 14 infants who underwent frenotomy. The mothers of the control group answered the questionnaire only at the time of the first assessment. Data were subjected to statistical analysis. Results : After frenotomy, the number of sucks increased and the pause length between sucking decreased during breastfeeding. The controls maintained the same patterns observed in the first assessment. From the questionnaire answered by the mothers of the 14 tongue-tied infants, at 30 days and 75 days, we observed that the symptoms concerning breastfeeding and sucking/swallowing/breathing coordination were improved after lingual frenotomy Conclusions : after lingual frenotomy, changes were observed in the breastfeeding patterns of the the tongue-tied infants while the control group maintained the same patterns. Moreover, all symptoms reported by the mothers of the tongue-tied infants had improved after frenotomy
In situ effect of a proanthocyanidin mouthrinse on dentin subjected to erosion
Proanthocyanidin has been shown to be efficient in inhibiting matrix metalloproteinases. Objective: The aim of this in situ study was to evaluate the protective effect of Proanthocyanidin-based mouthrinses either with naturally acidic or with a neutral pH applied on dentin subjected to erosion. Methodology: Eight volunteers wore one palatal device in two phases (7 days washout) with 16 samples per group (n=8). The groups under study were: First Phase/ G1 – 10% proanthocyanidin mouthrinse (pH 7.0, Experimental group 1 – Purified Grape Seeds Oligomeric Proanthocyanidins), G2 – 10% proanthocyanidin mouthrinse (pH 3.0, Experimental group 2 – Purified Grape Seeds Oligomeric Proanthocyanidins). Second Phase/ G3 – 0.12% chlorhexidine mouthrinse (pH 7.0, Positive control group), G4 – no previous treatment (Negative control group). Each device was subjected to 3 erosive cycles (5 minutes) per day for 5 days. Treatments with different mouthrinses were applied once after the second erosive challenge (5 minutes). Profilometry was used to quantify dentin loss (µm). Results: Data were analyzed by repeated measures of ANOVA followed by Fisher’s test (p<0.05). G1 (1.17±0.69) and G3 (1.22±0.25) showed significantly lower wear values with no statistical difference between them. G2 (2.99±1.15) and G4 (2.29±1.13) presented higher wear values with no significant differences between them. Conclusion: The 10% proanthocyanidin mouthrinse (pH 7.0) could be a good strategy to reduce dentin wear progression
The effects of frenotomy on breastfeeding
Although the interference of tongue-tie with breastfeeding is a controversial subject, The use of lingual frenotomy has been widely indicated by health professionals. To observe changes in breastfeeding patterns after lingual frenotomy concerning the number of sucks, pause length between groups of sucking and mother's complaints. Oral yes/no questions about breastfeeding symptoms and sucking/swallowing/breathing coordination were answered by the mothers of 109, 30 day old infants. On the same day the infants had their lingual frenulum assessed by administering a lingual frenulum protocol. After the assessment, all tongue-tied infants were referred for frenotomy; nevertheless, only 14 underwent the surgery. Of the 109 infants, 14 infants who did not have frenulum alterations were included as controls. Birth order and gender were the criteria for recruiting the control group. The tongue-tied infants underwent lingual frenotomy at 45 days of age. At the conclusion of the frenotomy, the infants were breastfed. At 75 days old, both groups – control and post-frenotomy – were reassessed. Before the reassessment the same oral yes/no questions were answered by the mothers of the 14 infants who underwent frenotomy. The mothers of the control group answered the questionnaire only at the time of the first assessment. Data were subjected to statistical analysis. After frenotomy, the number of sucks increased and the pause length between sucking decreased during breastfeeding. The controls maintained the same patterns observed in the first assessment. From the questionnaire answered by the mothers of the 14 tongue-tied infants, at 30 days and 75 days, we observed that the symptoms concerning breastfeeding and sucking/swallowing/breathing coordination were improved after lingual frenotomy. After lingual frenotomy, changes were observed in the breastfeeding patterns of the the tongue-tied infants while the control group maintained the same patterns. Moreover, all symptoms reported by the mothers of the tongue-tied infants had improved after frenotomy.23215315
Phenotype and cell proliferation activity of duct-like structures in human sublingual glands: a histological and immunohistochemical study
There are several age-related microscopic changes in the salivary glands, including the increase in the number of duct-like structures (DLS). However, the true origin and the phenotype of the DLS are not known. Objective To evaluate the phenotype and the cell proliferation index of the DLS of human sublingual glands. Material and Methods Sixty sublingual glands obtained from human cadavers were divided into two groups - 0-30 and 61-90 years old. The phenotype was estimated by immunostaining for cytokeratin 19 (CK 19) and the S-100 protein as well as by the presence of mucin and glycogen. The cell proliferation index was determined by the Ki-67 antibody. The histochemical techniques used periodic acid-Schiff (PAS) and Alcian Blue. In each captured microscopic field, the DLS were counted to establish a percentage for the staining profile. The statistical analysis was accomplished using Student's t-test, the Mann-Whitney test and Pearson's correlation coefficient (
CO2 laser and/or fluoride enamel treatment against in situ/ex vivo erosive challenge
Objective This in situ/ex vivo study investigated the effect of CO2 laser irradiation and acidulated phosphate fluoride gel (APF) application, separately and in combination, on enamel resistance to erosion. Material and Methods During 2 experimental 5-day crossover phases, 8 volunteers wore intraoral appliances containing bovine enamel blocks which were submitted to four groups: 1st phase - control, untreated and CO2 laser irradiation, 2nd phase - fluoride application and fluoride application before CO2 laser irradiation. Laser irradiation was performed at 10.6 µm wavelength, 5 µs pulse duration and 50 Hz frequency, with average power input and output of 2.3 W and 2.0 W, respectively (28.6 J/cm2). APF gel (1.23%F, pH 3.5) was applied on enamel surface with a microbrush and left on for 4 minutes. Then, the enamel blocks were fixed at the intraoral appliance level. The erosion was performed extraorally 4 times daily for 5 min in 150 mL of cola drink. Enamel loss was measured profilometrically after treatment and after the in situ phase. The data were tested using one-way Repeated Measures Anova and Tukey's test (
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