Constraints on the kinetic mixing parameter ϵ2\epsilon^2 for the light dark photons from dilepton production in heavy-ion collisions in the few-GeV energy range

The vector $U$-bosons, or so called 'dark photons', are one of the possible candidates for the dark matter mediators. They are supposed to interact with the standard matter via a 'vector portal' due to the $U(1)-U(1)^\prime$ symmetry group mixing which might make them visible in particle and heavy-ion experiments. While there is no confirmed observation of dark photons, the detailed analysis of different experimental data allows to estimate the upper limit for the kinetic mixing parameter $\epsilon^2$ depending on the mass $M_U$ of $U$-bosons which is also unknown. In this study we present theoretical constraints on the upper limit of $\epsilon^2(M_U)$ in the mass range $M_U \le 0.6$ GeV from the comparison of the calculated dilepton spectra with the experimental data from the HADES Collaboration at SIS18 energies where the dark photons are not observed. Our analysis is based on the microscopic Parton-Hadron-String Dynamics (PHSD) transport approach which reproduces well the measured dilepton spectra in $p+p$, $p+A$ and $A+A$ collisions. Additionally to the different dilepton channels originating from interactions and decays of ordinary matter particles (mesons and baryons), we incorporate the decay of hypothetical $U$-bosons to dileptons, $U\to e^+e^-$, where the $U$-bosons themselves are produced by the Dalitz decay of pions $\pi^0\to \gamma U$, $\eta$-mesons $\eta \to \gamma U$ and Delta resonances $\Delta \to N U$. Our analysis can help to estimate the requested accuracy for future experimental searches of 'light' dark photons by dilepton experiments.Comment: 11 pages, 5 figures, extended version, to be published in Phys. Rev.

Semantic URL Analytics to Support Efficient Annotation of Large Scale Web Archives

Long-term Web archives comprise Web documents gathered over longer time periods and can easily reach hundreds of terabytes in size. Semantic annotations such as named entities can facilitate intelligent access to the Web archive data. However, the annotation of the entire archive content on this scale is often infeasible. The most efficient way to access the documents within Web archives is provided through their URLs, which are typically stored in dedicated index files. The URLs of the archived Web documents can contain semantic information and can offer an efficient way to obtain initial semantic annotations for the archived documents. In this paper, we analyse the applicability of semantic analysis techniques such as named entity extraction to the URLs in a Web archive. We evaluate the precision of the named entity extraction from the URLs in the Popular German Web dataset and analyse the proportion of the archived URLs from 1,444 popular domains in the time interval from 2000 to 2012 to which these techniques are applicable. Our results demonstrate that named entity recognition can be successfully applied to a large number of URLs in our Web archive and provide a good starting point to efficiently annotate large scale collections of Web documents

West Nile virus: characterization and diagnostic applications of monoclonal antibodies

<p>Abstract</p> <p>Background</p> <p>Diagnosis of West Nile virus (WNV) infections is often difficult due to the extensive antigenic cross-reactivity among flaviviruses, especially in geographic regions where two or more of these viruses are present causing sequential infections. The purpose of this study was to characterize a panel of monoclonal antibodies (MAbs) produced against WNV to verify their applicability in WNV diagnosis and in mapping epitope targets of neutralizing MAbs.</p> <p>Methods</p> <p>Six MAbs were produced and characterized by isotyping, virus-neutralization, western blotting and MAb-epitope competition. The MAb reactivity against various WNVs belonging to lineage 1 and 2 and other related flaviviruses was also evaluated. The molecular basis of epitopes recognized by neutralizing MAbs was defined through the selection and sequencing of MAb escape mutants. Competitive binding assays between MAbs and experimental equine and chicken sera were designed to identify specific MAb reaction to epitopes with high immunogenicity.</p> <p>Results</p> <p>All MAbs showed stronger reactivity with all WNVs tested and good competition for antigen binding in ELISA tests with WNV-positive equine and chicken sera. Four MAbs (3B2, 3D6, 4D3, 1C3) resulted specific for WNV, while two MAbs (2A8, 4G9) showed cross-reaction with Usutu virus. Three MAbs (3B2, 3D6, 4D3) showed neutralizing activity. Sequence analysis of 3B2 and 3D6 escape mutants showed an amino acid change at E307 (Lys → Glu) in the E protein gene, whereas 4D3 variants identified mutations encoding amino acid changed at E276 (Ser → Ile) or E278 (Thr → Ile). 3B2 and 3D6 mapped to a region on the lateral surface of domain III of E protein, which is known to be a specific and strong neutralizing epitope for WNV, while MAb 4D3 recognized a novel specific neutralizing epitope on domain II of E protein that has not previously been described with WNV MAbs.</p> <p>Conclusions</p> <p>MAbs generated in this study can be applied to various analytical methods for virological and serological WNV diagnosis. A novel WNV-specific and neutralizing MAb (4D3) directed against the unknown epitope on domain II of E protein can be useful to better understand the role of E protein epitopes involved in the mechanism of WNV neutralization.</p

Search for dark photons in heavy‐ion collisions

The vector $U$-bosons, or so called 'dark photons', are one of the possible candidates for the dark matter (DM) mediators. They are supposed to interact with the standard matter via a 'vector portal' due to the $U(1)-U(1)^\prime$ symmetry group mixing which might make them visible in particle and heavy-ion experiments. While there is no confirmed observation of dark photons, the detailed analysis of different experimental data allows to estimate the upper limit for the kinetic mixing parameter $\epsilon^2$ depending on the mass $M_U$ of $U$-bosons which is also unknown. We have introduced a procedure to define theoretical constraints on the upper limit of $\epsilon^2(M_U)$ from heavy-ion (as well as p+p and $p+A$) dilepton data. Our analysis is based on the microscopic Parton-Hadron-String Dynamics (PHSD) transport approach where we incorporated the decay of hypothetical $U$-bosons to dileptons, $U\to e^+e^-$, where the $U$-bosons themselves are produced by the Dalitz decay of pions $\pi^0\to \gamma U$, $\eta$-mesons $\eta \to \gamma U$ and Delta resonances $\Delta \to N U$. The extension of our procedure to other dark matter candidates is foreseen

Constraints on the kinetic mixing parameter ε2^2 for the light dark photons from dilepton production in heavy-ion collisions in the few-GeV energy range

The vector $U$-bosons, or so called 'dark photons', are one of the possible candidates for the dark matter mediators. They are supposed to interact with the standard matter via a 'vector portal' due to the $U(1)-U(1)^\prime$ symmetry group mixing which might make them visible in particle and heavy-ion experiments. While there is no confirmed observation of dark photons, the detailed analysis of different experimental data allows to estimate the upper limit for the kinetic mixing parameter $\epsilon^2$ depending on the mass $M_U$ of $U$-bosons which is also unknown. In this study we present theoretical constraints on the upper limit of $\epsilon^2(M_U)$ in the mass range $M_U \le 0.6$ GeV from the comparison of the calculated dilepton spectra with the experimental data from the HADES Collaboration at SIS18 energies where the dark photons are not observed. Our analysis is based on the microscopic Parton-Hadron-String Dynamics (PHSD) transport approach which reproduces well the measured dilepton spectra in $p+p$, $p+A$ and $A+A$ collisions. Additionally to the different dilepton channels originating from interactions and decays of ordinary matter particles (mesons and baryons), we incorporate the decay of hypothetical $U$-bosons to dileptons, $U\to e^+e^-$, where the $U$-bosons themselves are produced by the Dalitz decay of pions $\pi^0\to \gamma U$, $\eta$-mesons $\eta \to \gamma U$ and Delta resonances $\Delta \to N U$. Our analysis can help to estimate the requested accuracy for future experimental searches of 'light' dark photons by dilepton experiments

Morphological, Ecological and Molecular Studies of <i>Vannella simplex</i> Wohlfarth-Bottermann 1960 (Lobosea, Gymnamoebia), with a new Diagnosis of this Species

Vannella simplex (Gymnamoebia, Vannellidae) is one of the most common amoebae species, recorded from a variety of regions. It was originally described as a freshwater species, but has also been reported from shallow-water regions of the Baltic Sea. In the present work, we investigated the morphology and biology of three V. simplex isolates, originating from geographically distant regions. Among them is one brackish water strain, isolated from artificial cyanobacterial mats, which were originally sampled in Nivå Bay (Baltic Sea, The Sound). The strain is cyst-forming and can thrive at salinity ranges from 0–50 ppt. Phylogenetic relationships were investigated by sequencing partial SSU rDNA of the cultured V. simplex isolates. Additional sequences were obtained from four environmental DNA extractions of sediment samples collected from different localities in Switzerland. Analysis of all obtained sequences revealed a monophyletic group. Based on the analysis and comparison of morphological, ecological and molecular data sets we compiled a distribution map of V. simplex and propose an emendation of this species

Analysing and Enriching Focused Semantic Web Archives for Parliament Applications

The web and the social web play an increasingly important role as an information source for Members of Parliament and their assistants, journalists, political analysts and researchers. It provides important and crucial background information, like reactions to political events and comments made by the general public. The case study presented in this paper is driven by two European parliaments (the Greek and the Austrian parliament) and targets an effective exploration of political web archives. In this paper, we describe semantic technologies deployed to ease the exploration of the archived web and social web content and present evaluation results

Evaluation of two rapid antigen tests to detect SARS-CoV-2 in a hospital setting

Successful containment strategies for the SARS-CoV-2 pandemic will depend on reliable diagnostic assays. Point-of-care antigen tests (POCT) may provide an alternative to time-consuming PCR tests to rapidly screen for acute infections on site. Here, we evaluated two SARS-CoV-2 antigen tests: the STANDARD™ F COVID-19 Ag FIA (FIA) and the SARS-CoV-2 Rapid Antigen Test (RAT). For diagnostic assessment, we used a large set of PCR-positive and PCR-negative respiratory swabs from asymptomatic and symptomatic patients and health care workers in the setting of two University Hospitals in Munich, Germany, i.e. emergency rooms, patient care units or employee test centers. For FIA, overall clinical sensitivity and specificity were 45.4% (n = 381) and 97.8% (n = 360), respectively, and for RAT, 50.3% (n = 445) and 97.7% (n = 386), respectively. For primary diagnosis of asymptomatic and symptomatic individuals, diagnostic sensitivities were 60.9% (FIA) (n = 189) and 64.5% (RAT) (n = 256). This questions these tests' utility for the reliable detection of acute SARS-CoV-2-infected individuals, in particular in high-risk settings. We support the proposal that convincing high-quality outcome data on the impact of false-negative and false-positive antigen test results need to be obtained in a POCT setting. Moreover, the efficacy of alternative testing strategies to complement PCR assays must be evaluated by independent laboratories, prior to widespread implementation in national and international test strategies