Implementation and performance of SIBYLS: a dual endstation small-angle X-ray scattering and macromolecular crystallography beamline at the Advanced Light Source.

The SIBYLS beamline (12.3.1) of the Advanced Light Source at Lawrence Berkeley National Laboratory, supported by the US Department of Energy and the National Institutes of Health, is optimized for both small-angle X-ray scattering (SAXS) and macromolecular crystallography (MX), making it unique among the world's mostly SAXS or MX dedicated beamlines. Since SIBYLS was commissioned, assessments of the limitations and advantages of a combined SAXS and MX beamline have suggested new strategies for integration and optimal data collection methods and have led to additional hardware and software enhancements. Features described include a dual mode monochromator [containing both Si(111) crystals and Mo/B(4)C multilayer elements], rapid beamline optics conversion between SAXS and MX modes, active beam stabilization, sample-loading robotics, and mail-in and remote data collection. These features allow users to gain valuable insights from both dynamic solution scattering and high-resolution atomic diffraction experiments performed at a single synchrotron beamline. Key practical issues considered for data collection and analysis include radiation damage, structural ensembles, alternative conformers and flexibility. SIBYLS develops and applies efficient combined MX and SAXS methods that deliver high-impact results by providing robust cost-effective routes to connect structures to biology and by performing experiments that aid beamline designs for next generation light sources

The novel MAPT mutation K298E:mechanisms of mutant tau toxicity, brain pathology and tau expression in induced fibroblast-derived neurons

Frontotemporal lobar degeneration (FTLD) consists of a group of neurodegenerative diseases characterized by behavioural and executive impairment, language disorders and motor dysfunction. About 20-30 % of cases are inherited in a dominant manner. Mutations in the microtubule-associated protein tau gene (MAPT) cause frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17T). Here we report a novel MAPT mutation (K298E) in exon 10 in a patient with FTDP-17T. Neuropathological studies of post-mortem brain showed widespread neuronal loss and gliosis and abundant deposition of hyperphosphorylated tau in neurons and glia. Molecular studies demonstrated that the K298E mutation affects both protein function and alternative mRNA splicing. Fibroblasts from a skin biopsy of the proband taken at post-mortem were directly induced into neurons (iNs) and expressed both 3-repeat and 4-repeat tau isoforms. As well as contributing new knowledge on MAPT mutations in FTDP-17T, this is the first example of the successful generation of iNs from skin cells retrieved post-mortem

Biological baseline data, Youngs Bay, Oregon, 1974

This report presents biological baseline information gathered during the research project, "Physical, Chemical and Biological Studies on Youngs Bay." Youngs Bay is a shallow embayment located on the south shore of the Columbia River, near Astoria, Oregon. Nearby portions of the Youngs River, Lewis and Clark River, Columbia River, and Skipanon Waterway were also included in the study

Ecological and radioecological studies in the Columbia River Estuary and adjacent Pacific Ocean

This report represents the progress in "Ecological and Radioecological Studies in the Columbia River Estuary and Adjacent Pacific Ocean" for the period 1 April 1975 through 31 March 1976. This research has been supported with funds from the Division of Biomedical and Environmental Research, U.S. Energy Research and Development Administration through Contract AT(45-1)-2227, Task Agreement 12. Our current research activities are dealt with in a descriptive fashion in the section on Research in Progress. The Research Completed section of the report includes manuscripts which have been submitted for publication during the reporting period, as well as reprints of papers published during this same period

Biological effects data--fluoride and sulfur dioxide

The Alumax Pacific Aluminum Corporation has proposed construction of an aluminum reduction facility near Youngs Bay at Warrenton, Oregon. This report comprises one part of the final report to Alumax on a research project entitled, "Physical, Chemical and Biological Studies of Youngs Bay." It presents data pertaining to the potential biological effects of fluoride and sulfur dioxide, two potentially hazardous plant-stack emissions, on selected aquatic species of the area. Companion volumes provide a description of the physical characteristics (Boley et al., 1975), the geochemistry, (Johnson and Cutshall, 1975), and the aquatic animals present in Youngs Bay and adjacent ecosystems (Higley and Holton, 1975). An introductory volume provides general information and maps of the area, and summarizes the conclusions of all four studies (Slotta, Cutshall, and Holton, 1975). The primary objective of the biological effects study was to determine the effects of fluoride on selected estuarine organisms, including 1) determination of the levels of fluoride in the marine ecosystem that will prove toxic to important sport and commercial species or important species in their food chains; 2) examination of sublethal effects that fluorides added to the marine ecosystem will have on sport, commercial or foodchain species; 3) determination of the effect of fluorides on the rate of carbon fixation in phytoplankton. (Slotta et al. Research Proposal Addendum, 1973

Analysis of benthic infauna communities and sedimentation patterns of a proposed fill site and nearby regions in the Columbia River Estuary

The Port of Astoria has proposed to fill a 32.4 hectare inter- and subtidal area at the mouth of Youngs Bay, Columbia River, Oregon. The possible effects of this fill on the biota and sedimentation patterns of this area were studied from 31 August 1975 to 29 February 1976. Part I, the biological studies, analyzed the quantity of benthic life at the fill site in comparison to that in the lower 28 miles of the Columbia River estuary. The sediment textures of benthos samples were analyzed to determine sediment-fauna relations; salinity-temperature measurements were made at selected sites. Fish life at the fill site was also sampled to determine species composition and the relation of fish stomach contents to benthic life. Studies on sedimentation patterns (based on dredging records, photographs, and sediment samples taken in Slip 2 of the port docks) aimed at identifying undesirable sediment deposits which might occur because of the fill. The dominant benthic taxa at most stations were amphipods and polychaetes, although oligochaetes were abundant at some muddy stations. Amphipod densities in the lower river varied from about 200/m2 in deep areas to between 5,000 and 50,000/m2 in shallow, fine sediment areas such as Youngs Bay and extensive shoaling areas. These areas of high density, which include the fill site, were dominated by the tube-building amphipod Corophium. Twenty-five species of fish have been captured in this and previous work in Youngs Bay. Food habit studies have shown Corophium to be eaten in large quantities by many of these species. Using density estimates and river bathymetry as guides, it was very roughly estimated that 0.8 percent of the amphipod standing crop in the study area (CRM 0-28) occurred at the fill site, which represents 0.09 percent of this area. Net sediment transport seems to be from the Columbia River into Youngs Bay, but transport out of Youngs Bay does occur and may contribute greatly to sediment deposition in Slips 1 and 2. Sediment samples from Slip 2 were mud, but this changed abruptly to coarse sand at the slip mouth. This shows that slip sediments arise from suspended fine mud and not from coarser-grained bed-load sediments. There is little that can be done to prevent such deposition. The proposed extension would probably not affect this problem, but might alter the flow of water around the port. More extensive investigations of circulation and suspended sediment content should be made

Widespread Lewy body and tau accumulation in childhood and adult onset dystonia-parkinsonism cases with PLA2G6 mutations

AbstractThe 2 major types of neurodegeneration with brain iron accumulation (NBIA) are the pantothenate kinase type 2 (PANK2)-associated neurodegeneration (PKAN) and NBIA2 or infantile neuroaxonal dystrophy (INAD) due to mutations in the phospholipase A2, group VI (PLA2G6) gene. We have recently demonstrated clinical heterogeneity in patients with mutations in the PLA2G6 gene by identifying a poorly defined subgroup of patients who present late with dystonia and parkinsonism. We report the clinical and genetic features of 7 cases with PLA2G6 mutations. Brain was available in 5 cases with an age of death ranging from 8 to 36 years and showed widespread alpha-synuclein-positive Lewy pathology, which was particularly severe in the neocortex, indicating that the Lewy pathology spread corresponded to Braak stage 6 and was that of the “diffuse neocortical type”. In 3 cases there was hyperphosphorylated tau accumulation in both cellular processes as threads and neuronal perikarya as pretangles and neurofibrillary tangles. Later onset cases tended to have less tau involvement but still severe alpha-synuclein pathology. The clinical and neuropathological features clearly represent a link between PLA2G6 and parkinsonian disorders

1960: Abilene Christian College Lectures - Full Text

Table of Contents: Theme Speeches: Christian Faith in the Modern World Basis of Faith - Leonard Mullens - 9 Authority in Christianity - John T. Smithson, Jr. - 27 Origin and Preservation of the Bible - Neil R. Lightfoot - 44 Alleged Discrepancies of the Bible - David H. Bobo - 62 The Unity of the Bible - Jack Meyer - 91 Faith and Reason - Joe Sanders - 115 The Reasonableness of Supernaturalism - Virgil Trout - 126 The Present Statue of the Doctrine of Organic Evolution - J.D. Thomas - 146 The Nature of Man - Roy F. Osborne, Jr. - 181 Modern Challenges to Christian Morals - Carl Spain - 199 The Christ, Whose Son is He? - Gordon Teel - 232 Special Speeches Teaching the Word of God in Korea - L. Haskell Chessfire - 255 The Influence of Christian Education - Judge Jack Pope - 276 Mission Opportunities in the Far East - Harry Robert Fox - 288 Mission Work in Austria - Robert Skelton - 303 Report from Switzerland - Heinrich Blum - 313 The Work in Nigeria - Rees Byrant - 320 The Training of Evangelists in Foreign Fields - Reiner Kallus - 331 Christian Scholarships - Everett Ferguson - 340 Evangelizing the World - A.R. Holton - 349 Panel Discussions The Significance of the Dead Sea Scrolls The Scrolls and the Text of the Bible - Paul Rotenberry - 357 The Relation between the Religion of the Essenes and that of Early Christians - Jay Smith - 366 Biblical Interpretation Expediency and Pattern Authority - J.W. Roberts - 381 Examples in Pattern Authority - Thomas B. Warren - 392 Mental Health and Sin The Present State of Mental Health Knowledge - Donald R. Sime - 409 The Relationship of Mental Health Problems to Sin - Paul Easley - 421 The Teenager The Problems of Youth - Mack Wayne Craig - 432 Influences for Good - Wyatt Sawyer - 443 The Benefits of Abilene Christian College To the Church - Hulen Jackson - 451 To The Home - Robert S. Bell - 459 \u27To the Community - Louie Welch - 465 Expenses At Abilene Christian College - James C. Kerr - 469 The Graduate School at Abilene Christian College What I Am Getting Now in the ACC Graduate Program - Harold Vanderpool - 475 How the ACC Graduate Program Has Stood Up - Everett Ferguson - 481 What the ACC Graduate Program Ought To Be - Frank Pack - 486 The Importance to the Church of the ACC Graduate Program A.R. Holton - 490 Beware: Large File Size Uploaded by Jackson Hage

1965: Abilene Christian College Bible Lectures - Full Text

LIFT UP YOUR EYES” Being the Abilene Christian College Annual Bible Lectures 1965 Price: $3.95 Published by ABILENE CHRISTIAN COLLEGE STUDENTS EXCHANGE ACC Station Abilene. Texa