Widespread occurrence of Mycobacterium tuberculosis DNA from 18th-19th century Hungarians

A large number (265) of burials from 1731-1838 were discovered in sealed crypts of the Dominican Church, Vac, Hungary in 1994. Many bodies were naturally mummified, so that both soft tissues and bones were available. Contemporary archives enabled the determination of age at death, and the identification of family groups. In some cases, symptoms before death were described and, occasionally, occupation. Initial radiological examination of a small number of individuals had indicated calcified lung lesions and demonstrable acid-fast bacteria suggestive of tuberculosis infection. Tuberculosis was endemic in 18th-19th century Europe, so human remains should contain detectable Mycobacterium tuberculosis complex (MTB) DNA, enabling comparisons with modern isolates. Therefore, a comprehensive examination of 168 individuals for the presence of MTB DNA was undertaken. Specific DNA amplification methods for MTB showed that 55% of individuals were positive and that the incidence varied according to age at death and sampling site in the body. Radiographs were obtained from 27 individuals and revealed an association between gross pathology and the presence of MTB DNA. There was an inverse relationship between PCR positivity and MTB target sequence size. In some cases, the preservation of MTB DNA was excellent, and several target gene sequences could be detected from the same sample. This information, combined with MTB DNA sequencing data and molecular typing techniques, will enable us to study the past epidemiology of TB infection, and extends the timeframe for studying changes in molecular fingerprints. Am J Phys Anthropol 120:144-152, 2003. (C) 2003 Wiley-Liss, Inc

Deutsch-Jozsa algorithm as a test of quantum computation

A redundancy in the existing Deutsch-Jozsa quantum algorithm is removed and a refined algorithm, which reduces the size of the register and simplifies the function evaluation, is proposed. The refined version allows a simpler analysis of the use of entanglement between the qubits in the algorithm and provides criteria for deciding when the Deutsch-Jozsa algorithm constitutes a meaningful test of quantum computation.Comment: 10 pages, 2 figures, RevTex, Approved for publication in Phys Rev

The atmospheric effects of stratospheric aircraft: A current consensus

In the early 1970's, a fleet of supersonic aircraft flying in the lower stratosphere was proposed. A large fleet was never built for economic, political, and environmental reasons. Technological improvements may make it economically feasible to develop supersonic aircraft for current markets. Some key results of earlier scientific programs designed to assess the impact of aircraft emissions on stratospheric ozone are reviewed, and factors that must be considered to assess the environmental impact of aircraft exhaust are discussed. These include the amount of nitrogen oxides injected in the stratosphere, horizontal transport, and stratosphere/troposphere assessment models are presented. Areas in which improvements in scientific understanding and model representation must be made to reduce the uncertainty in model calculations are identified

What grounded theory is ... a critically reflective conversation among scholars

Grounded theory (GT) is taught in many doctoral schools across the world and exemplified in most methodological books and publications in top-tier journals as a qualitative research method. This limited view of GT does not allow full use of possible resources and restrains researchers’ creativity and capabilities. Thus, it blocks some innovative possibilities and the emergence of valuable theories, which are badly needed. Therefore, understanding the full reach and scope of GT is becoming urgent, and we brought together a panel of established grounded theory scholars to help us in this endeavor through a reflective conversation

A Priority-Based Admission Control Scheme for Commercial Web Servers

This paper investigates into the performance and load management of web servers that are deployed in commercial websites. Such websites offer various services such as flight/hotel booking, online banking, stock trading, and product purchases among others. Customers are increasingly relying on these round-the-clock services which are easier and (generally) cheaper to order. However, such an increasing number of customers’ requests makes a greater demand on the web servers. This leads to web servers’ overload and the consequential provisioning of inadequate level of service. This paper addresses these issues and proposes an admission control scheme which is based on the class-based priority scheme that classifies customer’s requests into different classes. The proposed scheme is formally specified using ΠΠ-calculus and is implemented as a Java-based prototype system. The prototype system is used to simulate the behaviour of commercial website servers and to evaluate their performance in terms of response time, throughput, arrival rate, and the percentage of dropped requests. Experimental results demonstrate that the proposed scheme significantly improves the performance of high priority requests but without causing adverse effects on low priority requests

Effect of a Single Bout of Prior Moderate Exercise on Cutaneous Perfusion in Type 2 Diabetes

In diabetic individuals, increased shunting of circulation away from the skin may exist, contributing to their greater risk for ulcerations and poor cutaneous healing. In a prospective study (1), we previously found a lower skin perfusion during local heating in the foot dorsum of sedentary type 2 diabetic individuals compared with active people without diabetes. This defect was present despite normal increases in skin interstitial nitric oxide (NO), suggesting that NO is either ineffective or not involved (2). A prior bout of maximal exercise also lessened the impaired responsiveness to local heating of the dorsal foot in active type 2 diabetic individuals but not in their sedentary counterparts (3). Thus, this study examined the effect of a single bout of prior moderate cycle exercise on dorsal foot cutaneous perfusion and interstitial NO

Cutaneous Blood Flow in Type 2 Diabetic Individuals After an Acute Bout of Maximal Exercise

OBJECTIVE - We previously demonstrated a positive association between chronic aerobic exercise and dorsal foot skin blood flow during local heating in type 2 diabetic individuals. Thus, we hypothesized that a prior acute bout of maximal exercise would also have positive effects on postexercise blood now. RESEARCH DESIGN AND METHODS - Subjects consisted of 32 individuals with type 2 diabetes and 26 nondiabetic control subjects further subdivided based on their physical activity status diabetic exerciser (DE), diabetic sedentary (DS), control exerciser (CE), or control sedentary. Dorsal foot cutaneous blood flow was measured noninvasively by continuous laser-Doppler assessment at baseline and during local heating to 44°C before and after a maximal bout of cycle exercise. Interstitial nitric oxide (NO) levels were measured concurrently in the foot dorsum. RESULTS - increases in blood flow and its responsiveness to local heating to 44°C were significantly lower in both diabetic groups compared with CE before maximal exercise, but perfusion responsiveness remained lower in DS subjects only after exercise (P \u3c 0.05). Baseline skin blood flow was not different among groups preexercise, but it was significantly increased postexercise in DE subjects only. Interstitial NO levels were not significantly different at either time. At baseline, groups differed only in HbA1c, fasting serum glucose, HDL cholesterol, and insulin resistance (homeostasis model assessment method). CONCLUSIONS - All diabetic individuals exhibit a blunted responsiveness of cutaneous blood flow with local heating to 44°C before maximal exercise compared with active nondiabetic individuals, but after an exercise bout, it remains significantly blunted only in diabetic individuals who are sedentary. These findings occur independently of changes in interstitial NO levels

Determination of eddy-diffusivity in the lowermost stratosphere

We present a 2D-advection-diffusion model that simulates the main transport pathways influencing tracer distributions in the lowermost stratosphere (LMS). The model describes slow diabatic descent of aged stratospheric air, vertical (cross-isentropic) and horizontal (along isentropes) diffusion within the LMS and across the tropopause using equivalent latitude and potential temperature coordinates. Eddy diffusion coefficients parameterize the integral effect of dynamical processes leading to small scale turbulence and mixing. They were specified by matching model simulations to observed CO distributions. Interestingly, the model suggests mixing across isentropes to be more important than horizontal mixing across surfaces of constant equivalent latitude, shining new light on the interplay between various transport mechanisms in the LMS. The model achieves a good description of the small scale tracer features at the tropopause with squared correlation coefficients R2 = 0.72…0.94

Excerpts from the paper: Research Status and Recommendation from the Alaska Workshop on Gravity Waves and Turbulence in the Middle Atmosphere, part 1.3A

Internal gravity waves are disturbances whose intrinsic frequencies k(c - u) are smaller than the Brunt-Vaisala frequency (N). Their importance arises because: they are the major components of the total flow and temperature variability fields of the mesosphere (i.e., shears and lapse rates) and hence constitute the likely sources of turbulence; and they are associated with fluxes of momentum that communicate stresses over large distances. For example, gravity waves exert a drag on the flow in the upper mesosphere. However, in order for gravity waves to exert a net drag on the atmosphere, they must be attenuated. There are two general types of processes that seek to attenuate gravity waves: dissipation and saturation. Dissipation is any process that is effective independent of the wave amplitude, while saturation occurs when certain wave amplitude conditions are met. Radiative damping is an example of dissipation, while convective overturning is an example of saturation. The two processes are not mutually exclusive

Coenzyme Q10 Levels Are Decreased in the Cerebellum of Multiple-System Atrophy Patients

BACKGROUND: The objective of this study was to evaluate whether the levels of coenzyme Q10 (CoQ10) in brain tissue of multiple system atrophy (MSA) patients differ from those in elderly controls and in patients with other neurodegenerative diseases. METHODS: Flash frozen brain tissue of a series of 20 pathologically confirmed MSA patients [9 olivopontocerebellar atrophy (OPCA) type, 6 striatonigral degeneration (SND) type, and 5 mixed type] was used for this study. Elderly controls (n = 37) as well as idiopathic Parkinson's disease (n = 7), dementia with Lewy bodies (n = 20), corticobasal degeneration (n = 15) and cerebellar ataxia (n = 18) patients were used as comparison groups. CoQ10 was measured in cerebellar and frontal cortex tissue by high performance liquid chromatography. RESULTS: We detected a statistically significant decrease (by 3-5%) in the level of CoQ10 in the cerebellum of MSA cases (P = 0.001), specifically in OPCA (P = 0.001) and mixed cases (P = 0.005), when compared to controls as well as to other neurodegenerative diseases [dementia with Lewy bodies (P<0.001), idiopathic Parkinson's disease (P<0.001), corticobasal degeneration (P<0.001), and cerebellar ataxia (P = 0.001)]. CONCLUSION: Our results suggest that a perturbation in the CoQ10 biosynthetic pathway is associated with the pathogenesis of MSA but the mechanism behind this finding remains to be elucidated