Composition and Nutritive Value of Corn Co-Products from Dry Milling Ethanol Plants

The South Dakota ethanol industry is rapidly expanding. As a direct result of this expansion it is expected that in excess of 500,000 tons of corn co-products will be available to livestock enterprises annually as a feed source. Processing methods are, in general terms, similar among dry milling ethanol plants. However, newer technology and small deviations of methods can alter the nutritive value of the co-products. Subsequently, diet formulations using general nutritive values may be inaccurate and cause production inefficiencies. The objective of this research project was to characterize the composition and nutritive value of corn co-products produced from several dry milling ethanol plants in the upper Midwest

Shearing Lambs Improves Growth Performance During Periods with Elevated Thermal Load

The purpose of this study was to determine if average daily gain (ADG) is improved in shorn lambs versus non-shorn lambs in the summer months in the upper Midwestern United States. Forty-nine purebred Hampshire and Columbia ram (n = 10 Hampshire and 4 Columbia) and ewe (n = 22 Hampshire and 13 Columbia) lambs were grouped by breed, sex, age (104 ± 1.7 days of age), and initial weight (109 ± 3.5 lbs) into shorn (n=26) and non-shorn (n=23) groups. After shearing (3 June 2004), shorn sheep had approximately 0.1 inches of wool-cover. Lambs were weighed 1, 29, and 57 days following shearing. During the first 28 day period following shearing (period 1), there was no difference in average daily gain between shorn and non-shorn lambs (0.8 ± 0.04 vs 0.82 ± 0.04 lbs/day, respectively). In the second 28 day period (period 2), shorn lambs had a greater average daily gain than non-shorn lambs (1.02 ± 0.06 vs 0.92 ± 0.06 lbs/day, respectively). Period 2 had greater mean (68.9 vs. 63.4 oF, respectively), minimum (59.7 vs 54.1oF, respectively), and maximum (78.2 vs. 73.8oF, respectively) daily ambient air temperature and greater mean humidity (73.97 vs. 68.2 %, respectively) than period 1. The calculated temperature humidity index (THI) was also greater in period 2 than period 1 (66.9 vs. 62.24, respectively). These data indicate that shorn lambs grow more rapidly than non-shorn lambs during periods of elevated temperature, humidity, and THI

Composition and Nutritive Value of Corn Fractions and Ethanol Co-products Resulting from a New Dry-milling Process

The development of a new dry-milling process for the production of corn ethanol has resulted in new feedstuffs. This process fractionates the corn kernel prior to fermentation. Pre-fermentation fractions include bran, germ, and endosperm. Post-fermentation fractions include dried distillers grains (DDG) and condensed distiller solubles (syrup). Proximate analysis was conducted on these fractions along with the parent corn sample. Equations were used to predict TDN and undegradable intake protein (UIP). These feeds differ substantially from historical dried distiller’s grains with solubles (DDGS). Feeding experiments will be necessary to confirm the results of the predicted feed values

Effects of an Anabolic Implant and Transport on Metabolic Status and Muscle Traits of Feedlot Steers

A study was repeated over 2 years to determine the effect of feeding different levels of dry distillers grains with solubles (DDGS) and wet distillers grains with solubles (WDGS) on the performance of finishing cattle. In each year, 120 steers (756 ± 22 lb) were weighed, and randomly allocated to 15 pens. The pens were then assigned to one of five treatments: 1) corn-soybean meal (CON); 2) 20% DDGS; 3) 20% WDGS; 4) 40% DDGS; or 5) 40% WDGS. The basal diet consisted of 10% alfalfa hay, 4% molasses, 2% supplement, 10.5% SBM and 73.5% cracked corn. The WDGS and DDGS were added to replace all the SBM and part of the cracked corn. Steers were fed these diets for 138 and 129 days in years 1 and 2, respectively. Body weight was recorded prior to feeding at the start of the trial and every 28 days. Steers were harvested at a commercial facility and carcass data were collected. No treatment x year interactions occurred, thus data were pooled over the 2 years. There was an interaction between wet vs. dry and 20% vs. 40% distillers grains with solubles (DGS) for cumulative DMI. Steers fed 20% and 40% DDGS had the highest DMI, but feeding 40% WDGS significantly depressed (P \u3c 0.01) DMI. Cumulative ADG was similar across all treatments; however, steers fed 40% DGS had greater G:F (P \u3c 0.05) than those fed 20% DGS, and those fed WDGS were more efficient (P \u3c 0.01) than those fed DDGS. Pooled carcass data showed that steers fed DGS had greater (P \u3c 0.01) 12th rib fat compared to CON resulting in steers fed DGS having greater (P \u3c 0.05) Yield Grades compared to CON steers. Steers fed 20% DDGS and 20% WDGS had numerically higher (P \u3c 0.05) marbling scores compared to steers fed CON, 40% DDGS and 40% WDGS. Hot carcass weight, ribeye area, and percent kidney, pelvic, and heart fat were similar across all treatments. In conclusion, feeding DDGS and WDGS at 20 and 40% of the diet DM can be used to replace SBM in finishing diets to achieve similar gains and efficiencies. However, Yield Grades were greater for steers fed DGS compared to those fed the corn-soybean meal diet

Effect of Feeding Schedule on Tympanic Temperature of Steer Calves During Winter

Angus steer calves (n=135) were used in a 55d feedlot growing study to investigate the effects of feeding schedule on tympanic temperature response when limit feeding. Steers were fed a high moisture ear corn diet (58 Mcal/cwt NEg) at 0900h (AM), 1500h (PM) or 50% at 0900h and 50% at 1500h (SPLIT) to allow for 2.50lb ADG. Climatic data were collected at 30 min intervals throughout the study via an on site automated weather station. Tympanic temperatures (TT) were collected every 30 min (5 steers/trt) for 5d (d44 to d48). Mean ambient temperature during the 5d TT collection period was -6.50F (-23.1 to 9.10F). After 55d, BW (802, 808 and 802lb), ADG (2.56, 2.67 and 2.52lb) and feed efficiency (5.73, 5.52 and 5.87) did not differ (P\u3e.10) between AM, PM and SPLIT respectively, but followed rankings of previous research. Diurnal TT patterns were assessed by separating the day into three periods based on mean hourly wind chills (44.2, 17.8 and 7.50F) for Period 1 (0800 to 1600h), Period 2 (1630 to 2100h) and Period 3 (2130 to 0730h) respectively. Peak TT occurred during Period 2 for AM (102.40F) and Period 3 for PM (103.20F). SPLIT fed steers exhibited TT peaks of 103.90F or greater in each period. These data indicate that by adjusting feeding schedule it is possible to alter the time at which peak TT may occur, so that peak TT coincides with colder periods of the day. Elevated TT across all periods for SPLIT suggests that these steers may have increased metabolic rate to maintain normal TT during extreme cold. Additional research is needed to explain the changes in TT and how feeding times may impact energy partitioning

Plasma antioxidants from chocolate

There is some speculation that dietary flavonoids from chocolate, in particular (-)epicatechin, may promote cardiovascular health as a result of direct antioxidant effects or through antithrombotic mechanisms. Here we show that consumption of plain, dark chocolate results in an increase in both the total antioxidant capacity and the (-)epicatechin content of blood plasma, but that these effects are markedly reduced when the chocolate is consumed with milk or if milk is incorporated as milk chocolate. Our findings indicate that milk may interfere with the absorption of antioxidants from chocolate in vivo and may therefore negate the potential health benefits that can be derived from eating moderate amounts of dark chocolate

Impact of Relocation and Weaning Stress on the Feedlot Performance of Steer Calves During the Receiving Period

The effect of ranch management on weaning and relocation stress was investigated using spring born steer calves from a single source. Steers on mature (≥ 4 yr of age) dams were weaned at shipment (NW) whiles steers on young (\u3c 4 yr of age) dams had been weaned and on feed at the ranch for 1 mo prior to shipment (PW). At shipment tympanic temperature (TT) loggers, an indicator of body temperature, were placed in 13 steers from each management group at shipment. Steers were transported together to the SDSU Research Feedlot (365 mi). Body weight after 21 d in the feedlot (670 vs 673 lb) and DMI (14.7 vs 14.9 lb) were similar (P \u3e 0.10) between PW and NW steers, respectively. Average daily gain (2.66 vs 3.17 lb) and feed efficiency (5.53 vs 4.74 lb) were higher (P \u3c 0.05) in NW steers. NW steers recorded higher TT (1.2 ºF; P \u3c 0.05) than PW steers duing loading and transportation from the ranch, indicating a greater initial stress associated with NW. During the first 4 d at the feedlot DMI was lower for NW steers (11.2 vs 6.1 lb) and was slightly below maintenance. There were no differences (P \u3e 0.05) in TT associated with this period of negative energy balance. Results indicate that pre-transit weaning, as a part of feedlot transition, did not add sufficient stress to alter TT compared to previously weaned calves

Phase Separation of FUS is Modulated by Methylation State of Cation-π Interactions and Interaction with TNPO1

Reversible phase separation, which underpins the role of FUS in ribonucleoprotein granules and other membrane-free organelles, is in part driven by the intrinsically disordered low complexity (LC) domain of FUS. Here, we report that cooperative cation-π interactions between tyrosines in the LC domain and arginines in structured C-terminal domains also contribute to phase separation. These interactions are modulated by post-translational arginine methylation, wherein arginine hypomethylation strongly promotes phase separation and gelation. Indeed, significant hypomethylation, which occurs in FUS-associated frontotemporal lobar degeneration, induces FUS condensation into stable intermolecular β-sheet-rich hydrogels that disrupt RNP granule function and impair new protein synthesis in neuron terminals. We show that transportin acts as a physiological molecular chaperone of FUS in neuron terminals, reducing phase separation and gelation of methylated and hypomethylated FUS, and rescuing protein synthesis. These results demonstrate how FUS condensation is physiologically regulated, and how perturbations in these mechanisms can lead to disease

PROTOCOL: In‐person interventions to reduce social isolation and loneliness: An evidence and gap map

Abstract This is the protocol for an evidence and gap map. The objectives are as follows: This EGM aims to map available evidence on the effects of in‐person interventions to reduce social isolation and/or loneliness across all age groups in all settings

Classification of Salmonella enterica of the (Para-)Typhoid Fever Group by Fourier-Transform Infrared (FTIR) Spectroscopy

Typhoidal and para-typhoidal Salmonella are major causes of bacteraemia in resource-limited countries. Diagnostic alternatives to laborious and resource-demanding serotyping are essential. Fourier transform infrared spectroscopy (FTIRS) is a rapidly developing and simple bacterial typing technology. In this study, we assessed the discriminatory power of the FTIRS-based IR Biotyper (Bruker Daltonik GmbH, Bremen, Germany), for the rapid and reliable identification of biochemically confirmed typhoid and paratyphoid fever-associated Salmonella isolates. In total, 359 isolates, comprising 30 S. Typhi, 23 S. Paratyphi A, 23 S. Paratyphi B, and 7 S. Paratyphi C, respectively and other phylogenetically closely related Salmonella serovars belonging to the serogroups O:2, O:4, O:7 and O:9 were tested. The strains were derived from clinical, environmental and food samples collected at different European sites. Applying artificial neural networks, specific automated classifiers were built to discriminate typhoidal serovars from non-typhoidal serovars within each of the four serogroups. The accuracy of the classifiers was 99.9%, 87.0%, 99.5% and 99.0% for Salmonella Typhi, Salmonella Paratyphi A, B and Salmonella Paratyphi C, respectively. The IR Biotyper is a promising tool for fast and reliable detection of typhoidal Salmonella. Hence, IR biotyping may serve as a suitable alternative to conventional approaches for surveillance and diagnostic purposes