Interaction, Identity & Social Class

Social class is a longstanding locus of sociological inquiry. Prior research has investigated the phenomenon in an array of domains through a myriad of methodologies and theoretical perspectives. The heterogeneity that is posed by previous studies empirically and theoretically, however, is predicated upon a homogenous set of epistemological and ontological assumptions. This has resulted in a number of programmatic, enduring omissions. Most notably, research has neglected how social class is conceptualised and made relevant by members in forms of talk-in-interaction. Aligning with the commitments of Ethnomethodology (EM), and using Conversation Analysis (CA) and Membership Categorisation Analysis (MCA) specifically, this thesis addresses this lacuna. Data are composed of ordinary, naturalistic forms of talk-in-interaction conducted synchronously in the English language over the last half-century (n=959). The empirical contributions of this research concern the ontological affordances, formulative possibilities and praxiological functions of two families of interactional practices that occasion the relevance of classed identities recurrently – namely, “membership categorisations” and “place references”. Chapter 4 addresses the former, canvassing the agentic, ontological and intersubjective dimensions of linguistically classed membership categories. Chapter 5 introduces the latter as a resource used to actuate classed identities in a designedly referential and metonymic faculty. Chapter 6 then recovers the activity of “accounting” for which both practices are employed across action-types; specifically, “assessments”, “complaints” and “teases”. The central objective of this thesis thus concerns the “respecification” of social class as a “members’ phenomenon”; one that is made relevant within ordinary instances of talk-in-interaction through a stable set of interactional practices in order to accomplish a diverse range of practical tasks. The thesis concludes with a review of several candidate lines of analysis for future EM/(M)CA inquiry that are anticipated by the findings of this research uniquely

Interaction, Identity & Social Class

Social class is a longstanding locus of sociological inquiry. Prior research has investigated the phenomenon in an array of domains through a myriad of methodologies and theoretical perspectives. The heterogeneity that is posed by previous studies empirically and theoretically, however, is predicated upon a homogenous set of epistemological and ontological assumptions. This has resulted in a number of programmatic, enduring omissions. Most notably, research has neglected how social class is conceptualised and made relevant by members in forms of talk-in-interaction. Aligning with the commitments of Ethnomethodology (EM), and using Conversation Analysis (CA) and Membership Categorisation Analysis (MCA) specifically, this thesis addresses this lacuna. Data are composed of ordinary, naturalistic forms of talk-in-interaction conducted synchronously in the English language over the last half-century (n=959). The empirical contributions of this research concern the ontological affordances, formulative possibilities and praxiological functions of two families of interactional practices that occasion the relevance of classed identities recurrently – namely, “membership categorisations” and “place references”. Chapter 4 addresses the former, canvassing the agentic, ontological and intersubjective dimensions of linguistically classed membership categories. Chapter 5 introduces the latter as a resource used to actuate classed identities in a designedly referential and metonymic faculty. Chapter 6 then recovers the activity of “accounting” for which both practices are employed across action-types; specifically, “assessments”, “complaints” and “teases”. The central objective of this thesis thus concerns the “respecification” of social class as a “members’ phenomenon”; one that is made relevant within ordinary instances of talk-in-interaction through a stable set of interactional practices in order to accomplish a diverse range of practical tasks. The thesis concludes with a review of several candidate lines of analysis for future EM/(M)CA inquiry that are anticipated by the findings of this research uniquely

Healthcare professionals’ assertions and women's responses during labour: A conversation analytic study of data from One born every minute

© 2016 Elsevier Ireland Ltd Objective Communication during labour is consequential for women's experience yet analyses of situated labour-ward interaction are rare. This study demonstrates the value of explicating the interactional practices used to initiate ‘decisions’ during labour. Methods Interactions between 26 labouring women, their birth partners and HCPs were transcribed from the British television programme, One Born Every Minute. Conversation analysis was used to examine how decisions were initiated and accomplished in interaction. Findings HCPs initiate decision-making using interactional practices that vary the ‘optionality’ afforded labouring women in the responsive turn. Our focus here is on the minimisation of optionality through ‘assertions’. An ‘assertive’ turn-design (e.g. ‘we need to…’) conveys strong expectation of agreement. HCPs assert decisions in contexts of risk but also in contexts of routine activities. Labouring women tend to acquiesce to assertions. Conclusion The expectation of agreement set up by an assertive initiating turn can reduce women's opportunities to participate in shared decision-making (SDM). Practice implications When decisions are asserted by HCPs there is a possible dissonance between the tenets of SDM in British health policy and what occurs in situ. This highlights an educational need for HCPs in how best to afford labouring women more optionality, particularly in low-risk contexts

Frequent Cross-Species Transmission of Parvoviruses among Diverse Carnivore Hosts

Although parvoviruses are commonly described in domestic carnivores, little is known about their biodiversity in nondomestic species. A phylogenetic analysis of VP2 gene sequences from puma, coyote, gray wolf, bobcat, raccoon, and striped skunk revealed two major groups related to either feline panleukopenia virus (“FPV-like”) or canine parvovirus (“CPV-like”). Crossspecies transmission was commonplace, with multiple introductions into each host species but, with the exception of raccoons, relatively little evidence for onward transmission in nondomestic species

Malpais spring virus is a new species in the genus vesiculovirus

BACKGROUND: Malpais Spring virus (MSPV) is a mosquito-borne rhabdovirus that infects a variety of wild and feral ungulates in New Mexico, including horses and deer. Although, initial serologic tests and electron microscopy at the time of isolation nearly 25 years ago provided evidence that MSPV is a novel virus, possibly related to vesiculoviruses, the virus still has not been approved as a new species. FINDINGS: Use of the illumina platform allowed us to obtain the complete genome of MSPV. Analysis of the complete 11019 nt genome sequence of the prototype 85-488NM strain of MSPV indicates that it encodes the five common rhabdovirus structural proteins (N, P, M, G and L) with alternative ORFs (> 180 nt) in the N, M and G genes, including a 249 nt ORF in the G gene predicted to encode a 9.26 kDa highly basic transmembrane protein. Although antigenically very distant, phylogenetic analysis of the L gene indicates that MSPV is most closely related to Jurona virus, also isolated from mosquitoes in Brazil, as well as a number of other vesiculoviruses. CONCLUSIONS: In sum, our analysis indicates MSPV should be classified as a member of the genus Vesiculovirus, family Rhabdoviridae. The complete genome sequence of MSPV will be helpful in the development of a reverse genetics system to study the unique aspects of this vesiculovirus in vivo and in vitro, and will assist development of specific diagnostic tests to study the epidemiology of MSPV infection

Comparative analysis of the complete genome sequence of the California MSW strain of myxoma virus reveals potential host adaptations

Myxomatosis is a rapidly lethal disease of European rabbits that is caused by myxoma virus (MYXV). The introduction of a South American strain of MYXV into the European rabbit population of Australia is the classic case of host-pathogen coevolution following cross-species transmission. The most virulent strains of MYXV for European rabbits are the Californian viruses, found in the Pacific states of the United States and the Baja Peninsula, Mexico. The natural host of Californian MYXV is the brush rabbit, Sylvilagus bachmani. We determined the complete sequence of the MSW strain of Californian MYXV and performed a comparative analysis with other MYXV genomes. The MSW genome is larger than that of the South American Lausanne (type) strain of MYXV due to an expansion of the terminal inverted repeats (TIRs) of the genome, with duplication of the M156R, M154L, M153R, M152R, and M151R genes and part of the M150R gene from the right-hand (RH) end of the genome at the left-hand (LH) TIR. Despite the extreme virulence of MSW, no novel genes were identified; five genes were disrupted by multiple indels or mutations to the ATG start codon, including two genes, M008.1L/R and M152R, with major virulence functions in European rabbits, and a sixth gene, M000.5L/R, was absent. The loss of these gene functions suggests that S. bachmani is a relatively recent host for MYXV and that duplication of virulence genes in the TIRs, gene loss, or sequence variation in other genes can compensate for the loss of M008.1L/R and M152R in infections of European rabbits.This work was funded in part by grant R01 AI093804 from the National Institute of Allergy and Infectious Diseases, National Institutes of Health. E.C.H. was supported by an NHMRC Australia Fellowship, and D.C.T. was supported by an ARC Future Fellowship

Genome scale evolution of myxoma virus reveals host-pathogen adaptation and rapid geographic spread

The evolutionary interplay between myxoma virus (MYXV) and the European rabbit (Oryctolagus cuniculus) following release of the virus in Australia in 1950 as a biological control is a classic example of host-pathogen coevolution. We present a detailed genomic and phylogeographic analysis of 30 strains of MYXV, including the Australian progenitor strain Standard Laboratory Strain (SLS), 24 Australian viruses isolated from 1951 to 1999, and three isolates from the early radiation in Britain from 1954 and 1955. We show that in Australia MYXV has spread rapidly on a spatial scale, with multiple lineages cocirculating within individual localities, and that both highly virulent and attenuated viruses were still present in the field through the 1990s. In addition, the detection of closely related virus lineages at sites 1,000 km apart suggests that MYXV moves freely in geographic space, with mosquitoes, fleas, and rabbit migration all providing means of transport. Strikingly, despite multiple introductions, all modern viruses appear to be ultimately derived from the original introductions of SLS. The rapidity of MYXV evolution was also apparent at the genomic scale, with gene duplications documented in a number of viruses. Duplication of potential virulence genes may be important in increasing the expression of virulence proteins and provides the basis for the evolution of novel functions. Mutations leading to loss of open reading frames were surprisingly frequent and in some cases may explain attenuation, but no common mutations that correlated with virulence or attenuation were identified.This work was funded in part by grant R01 AI093804 from the National Institute of Allergy and Infectious Diseases, National Institutes of Health. E.C.H. is funded by an NHMRC Australia Fellowship. D.C.T. is funded by an ARC Future Fellowship

Bostonia: The Boston University Alumni Magazine. Volume 9

Founded in 1900, Bostonia magazine is Boston University's main alumni publication, which covers alumni and student life, as well as university activities, events, and programs

Multiple reassortment events in the evolutionary history of H1N1 influenza A virus since 1918

The H1N1 subtype of influenza A virus has caused substantial morbidity and mortality in humans, first documented in the global pandemic of 1918 and continuing to the present day. Despite this disease burden, the evolutionary history of the A/H1N1 virus is not well understood, particularly whether there is a virological basis for several notable epidemics of unusual severity in the 1940s and 1950s. Using a data set of 71 representative complete genome sequences sampled between 1918 and 2006, we show that segmental reassortment has played an important role in the genomic evolution of A/H1N1 since 1918. Specifically, we demonstrate that an A/H1N1 isolate from the 1947 epidemic acquired novel PB2 and HA genes through intra-subtype reassortment, which may explain the abrupt antigenic evolution of this virus. Similarly, the 1951 influenza epidemic may also have been associated with reassortant A/H1N1 viruses. Intra-subtype reassortment therefore appears to be a more important process in the evolution and epidemiology of H1N1 influenza A virus than previously realized