Structure-activity relationships of synthetic analogs of jasmonic acid and coronatine on induction of benzo[c]phenanthridine alkaloid accumulation in Eschscholzia californica cell cultures

A facile test system based on the accumulation of benzo[c]phenanthridine alkaloids in Eschscholzia californica cell suspension culture (an indicator of defense gene activation) has been used to analyze a series of synthetic compounds for elicitor-like activity. Of the 200 jasmonic acid and coronatine analogs tested with this system, representative results obtained with 49 of them are presented here. The following can be summarized concerning structure-actvity relationships: there is a large degree of plasticity allowed at the C-3 of jasmonic acid in the activation of defense genes. The carbonyl moiety is not strictly required, but exocyclic double bond character appears necessary. The pentenyl side chain at C-2 cannot tolerate bulky groups at the terminal carbon and still be biologically active. Substitutions to the C-1' position are tolerated if they can potentially undergo beta-oxidation. Either an alkanoic acid or methyl ester is required at c-l, or a side chain that can be shortened by beta-oxidation or by peptidase hydrolysis. Coronatine and various derivatives thereof are not as effective as jasmonic acid, and derivatives in inducing benzo[c]phenanthridine alkaloid accumulation. Jasmonic acid rather than the octadecanoic precursors is therefore considered to be a likely signal transducer of defense gene activation in planta

Biomass equations for Brazilian semiarid caatinga plants Equações para estimar a biomassa de plantas da caatinga do semi-árido brasileiro

Allometric equations to estimate total aboveground alive biomass (B) or crown projection area (C) of ten caatinga species based on plant height (H) and/or stem diameter at ground level (DGL) or at breast height (DBH) were developed. Thirty plants of each species, covering the common range of stem diameters (3 to 50 cm), were measured (C, H, DGL, DBH), cut at the base, separated into parts, weighted and subsampled to determine dry biomass. Wood density (p) of the stem and the largest branches was determined. B, C, H and p ranged from 1 to 500 kg, 0.2 to 112 m², 1.3 to 11.8 m, and 0.45 to 1.03 g cm-3. Biomass of all 10 species, separately or together (excluding one cactus species), could be estimated with high coefficients of determination (R²) using the power equation (B = aDGLb) and DGL, DBH, H or combinations of diameter, height and density. Improvement by multiplying H and/or p to DGL or DBH was small. The mixed-species equation based only on DBH (valid up to 30 cm) had a = 0.173 and b = 2.295, similar to averages of these parameters found in the literature but slightly lower than most of those for humid tropical vegetation. Crown area was significantly related to diameter, height and biomass.<br>Equações alométricas foram desenvolvidas para estimar a biomassa aérea viva (B) e a área de projeção da copa (C) de dez espécies da caatinga, com base na altura da planta (H) e/ou do diâmetro do caule ao nível do solo (DNS) ou à altura do peito (DAP). Trinta plantas de cada espécie, cobrindo a faixa usual de diâmetros (3 a 50 cm), foram medidas (C, H, DNS, DAP), cortadas na base, separadas em partes, pesadas e subamostradas para determinação da biomassa seca. A densidade (p) da madeira dos caules e galhos maiores foi determinada. B, C, H e p variaram de 1 a 500 kg, 0,2 a 112 m², 1,3 a 11,8 m e 0,45 a 1,03 g cm-3. A biomassa das 10 espécies, separadamente ou em conjunto (exceto pela espécie de Cactaceae), foi estimada com alto coeficiente de determinação (R²), usando a equação de potência (B = aDNSb) e DNS, DAP ou combinações de diâmetro, altura e densidade. A melhora com a multiplicação de DNS ou DAP por H e/ou p foi pequena. A equação de DAP (válida até 30 cm) para o conjunto das nove espécies teve a = 0,173 e b = 2,295, semelhantes aos valores das médias das equações encontradas na literatura, mas um pouco abaixo dos referidos para vegetação tropical úmida. A projeção das copas foi significativamente relacionada com diâmetros do caule, alturas e biomassas

New evidence for large negative xylem pressures and their measurement by the pressure chamber method

International audienc

Lithospheric evolution in the wake of the Mendocino Triple Junction: structure of the San Andreas Fault system at 2 Ma

As the Mendocino triple junction (MTJ) moves northwards up the North American margin, the tectonic regime changes from subduction to strike-slip. For the first few million years following triple junction migration, the San Andreas Fault system consists of several strike-slip faults distributing deformation over a region ~ 150 km wide. This same region is expected to be affected by a slab gap beneath North America, created by the northward removal of the subducting Gorda plate, and into which asthenospheric mantle is thought to rise to crustal depths. The onshore and offshore Mendocino Triple Junction Seismic Experiment (MTJSE) provides a continuous seismic velocity-reflectivity cross-section across the deforming zone from the Pacific ocean basin to the eastern edge of the California Coast Ranges. The accretionary complex rocks that make up most of the crustal thickness are underlain by a 5-10 km thick high-velocity(6.4-7.2 km s- 1) layer at the base of the crust that extends from the Pacific to at least 50 km, and probably 90 km east of the San Andreas Fault. The top of the lower crustal layer deepens from 7 km beneath the Pacific ocean basin at the west end of the profile to 23 km at the east end by a gentle (5° -10°) eastward dip punctuated by abrupt offsets at the San Andreas and Maacama fault zones. At each fault the top of the lower crust is offset by up to 4 km, down to the east. The Moho is similarly deformed beneath the faults, although by only 2 km. Such localized deformation of the Moho implies that these two strike-slip faults penetrate through the entire crust to the upper mantle. Good agreement between seismic velocity and seismic reflectivity in the vicinity of the faults gives confidence in these results, although details of the offset beneath the San Andreas Fault are better resolved than those under the Maacama Fault. Seismic velocities in the upper mantle show only a small change along the profile, from 8.1 km s- 1 beneath the Pacific to about 7.9 km s- 1 beneath the Coast Ranges. We infer that upwelling of asthenosphere into the slab gap is limited laterally, or a lithospheric lid is present in the slab gap by 2 Ma. Gravity data and crustal density structure show that most of the margin width is in local Airy isostasy with the changes in crustal thickness near the strike-slip faults corresponding closely to changes in surface topography. The crustal blocks defined by the strike-slip faults appear to be independently in isostatic equilibrium, provided that the mantle beneath the Coast Ranges has a somewhat lower density than that beneath the Pacific plate. The densities in the Coast Range upper mantle are consistent with limited temperature elevation, suggesting that the asthenospheric mantle is present beneath the depth of seismic energy penetration from our survey