Ti3C2Tx MXene-Au nanoparticles doped polyimide thin film as a transducing bioreceptor for real-time acoustic detection of carcinoembryonic antigen

Ti3C2Tx MXene was synthesized and the 2D layers were hybridized with Au nanoparticle(AuNP) through the in-situ method. The study was successfully prepared MXene 2D layerand the same was TEM captured as well. Polyimide/MXene-AuNP (PI/Mxene-AuNP)transducing conducting nanocomposite thin film was synthesized and evaluated the newmaterial’s capability to use as a transducing thin film for biosensing application. Thebioreceptor was prepared on the delay line area of the SAW device by covalentlyimmobilizing mouse monoclonal antibody of carcinoembryonic antigen (CEA) through thethioglycolic acid arm linker mechanism. Immunoassay analysis has suggested that thebiosensor responds linearly with the increase in the concentration of the CEA sample. Thelimit of detection was observed at 0.001 ng/ml. The insertion loss of the bioreceptor wasrecorded at 10 dB, which also mattered in the high sensitivity of the biosensor. The biosensorhas shown excellent selectivity within the environment of other common tumour markers andwas stable for 75 days under periodical testing conditions. Clinical serum samples wereanalyzed successfully and the results were compared with values obtained through the ELISAmethod

<i>ansa</i>-Ferrocene-Incorporated Calixpyrroles and Calixphyrins: Syntheses and Spectral/Structural Characterization

The syntheses and spectral/structural characterization of <i>ansa</i>-ferrocene-incorporated <i>normal</i> calixphyrins and <i>core-modified</i> calixpyrroles and calixphyrins are reported. Acid-promoted dehydrative condensation of 1,1′-bis­(dimethylpyrrolylmethyl)­ferrocene and 2,5-bis­(dimethylhydroxymethyl)­thiophene/furan yielded <i>ansa</i>-ferrocene-based <i>core-modified</i> calixpyrroles, while acid-catalyzed dehydrative condensation of 1,1′-bis­(diphenylpyrrolylmethyl)­ferrocene with the aryl aldehydes and 2,5-bis­(phenylhydroxymethyl)­thiophene followed by DDQ oxidation resulted in the formation of <i>ansa</i>-ferrocene-appended <i>normal</i> and <i>core-modified</i> calixphyrins, respectively. The newly synthesized macrocycles were characterized by FAB-MS, NMR, and UV–vis spectral analyses and finally confirmed by single-crystal X-ray structural analysis. All these studies clearly revealed the introduction of ferrocene in the main framework of the corresponding macrocycles in an <i>ansa</i>-type way. The <i>core-modified</i> calixpyrroles adopt a 1,3-alternate conformation, while the corresponding calixphyrins maintained partial planarity along the tripyrrin plane due to the presence of <i>meso</i> sp² carbon and generated curved staircase conformation. In addition to the intramolecular hydrogen-bonding interactions, calixphyrins generate self-assembled dimers, one- and two-dimensional supramolecular assemblies through intermolecular hydrogen bonding in the solid state