Réflexion non exhaustive sur les apports des réseaux et d'Internet dans l'exercice officinal

TOURS-BU Sciences Pharmacie (372612104) / SudocSudocFranceF

Le E-commerce des médicaments en Europe en 2005

Le commerce électronique permet à priori l'échange mondial de tout produit matériel, sans distinction aucune. Pourtant, si certaines marchandises peuvent être commercialisées en ligne sans restriction majeure, d'autres, considérées à risque, font l'objet de législations moins permissives. Tel est le cas des médicaments, objets d'un cadre juridique national et communautaire très strict. Celui-ci prend en compte le double enjeu constitué par la protection du consommateur et les impératifs de santé. Dans certains pays d'Europe, on assiste déjà à l'émergence d'officines virtuelles, les " cyber-pharmacies ". Mais quelle est la légalité de ces nouvelles institutions en droit français à l'heure actuelle ? La vente en ligne de médicaments est-elle licite en France?TOURS-BU Sciences Pharmacie (372612104) / SudocSudocFranceF

Les Statistiques (un outil essentiel de gestion et d'aide à la décision pour le pharmacien d'officine)

TOURS-BU Sciences Pharmacie (372612104) / SudocSudocFranceF

Réalisations et perpectives du e-commerce pour l'industrie pharmaceutique (le cas direct medica. Implications pour la pharmacie d'officine)

TOURS-BU Sciences Pharmacie (372612104) / SudocSudocFranceF

Validation des systèmes informatisés (cas pratique d'un système de gestion des cahiers de laboratoire)

Ma thèse porte sur la validation des systèmes informatisés avec comme cas pratique la validation d un système de gestion des cahiers de laboratoire au sein du service analytique de Bertin Pharma (laboratoire sous-traitant de R&D pharmaceutique, situé à Artigues-près-Bordeaux (33)). L'environnement réglementaire (GMP / BPF) exige que les systèmes informatisés mis en œuvre dans le cadre d'opérations BPF soient validés. La validation de ce système est réalisée à travers trois phases de qualification qui sont la qualification d'installation, la qualification opérationnelle et la qualification de performance. Ce système, qui est une application intranet, permettra la réalisation de différentes fonctions nécessaires à l activité de la société Bertin Pharma.TOURS-BU Sciences Pharmacie (372612104) / SudocSudocFranceF

: beta-carotene, alpha-linolenate and carcinogenesis.

To investigate whether dietary alpha-linolenic acid (ALA) content alters the effect of beta-carotene on mammary carcinogenesis, we conducted a chemically induced mammary tumorigenesis experiment in rats randomly assigned to four nutritional groups (15 rats per group) varying in beta-carotene supplementation and ALA content. Two oil formula-enriched diets (15 %) were used: one with 6 g ALA/kg diet in an essential fatty acids (EFA) ratio of linoleic acid:ALA of 5:1 w/w (EFA 5 diet), the other with 24 g ALA/kg diet in an EFA ratio of 1:1 w/w (EFA 1 diet), both designed with a similar linoleic acid content. beta-Carotene was either added (10 mg/kg diet per d) or not added to these diets. beta-Carotene supplementation led to decreased tumour incidence and tumour growth when added to the EFA 5 diet, whereas it had no effect when added to the EFA 1 diet. The decreased tumour growth did not result from an involvement of lipoperoxidation (tumour malondialdehyde content being similar between the groups) or from an inhibition of tumour cell proliferation (as there was an unchanged S phase fraction in the tumours). We concluded that an adequate content of ALA in the diet is required to allow a protective effect of beta-carotene in mammary carcinogenesis. Whether such an interaction between ALA and beta-carotene influences the risk of breast cancer in women needs to be investigated

Heat inactivation of monkeypox virus

Different kinds of media spiked with monkeypox virus (MPXV) were subjected to heat inactivation at different temperatures for various periods of time. The results showed that MPXV was inactivated in less than 5 min at 70 °C and less than 15 min at 60 °C, with no difference between viruses from the West African and Central African clades. The present findings could help laboratory workers to manipulate MPXV in optimal biosafety conditions and improve their protocols

Heat inactivation of monkeypox virus

International audienceDifferent kinds of media spiked with monkeypox virus (MPXV) were subjected to heat inactivation at different temperatures for various periods of time. The results showed that MPXV was inactivated in less than 5 min at 70 °C and less than 15 min at 60 °C, with no difference between viruses from the West African and Central African clades. The present findings could help laboratory workers to manipulate MPXV in optimal biosafety conditions and improve their protocols

Gut emptying affects dietary fat contribution to postprandial lipemia following sequential meals in healthy subjects

International audienceOBJECTIVE: The present study examined the kinetic of plasma triacylglycerol (TAG) and gut emptying after sequential ingestion of breakfast and lunch, and the contribution of dietary fat ingested at breakfast to subsequent TAG after lunch. METHODS: Nine subjects ingested a breakfast (0730 h) and a lunch (1200 h) containing 25 and 44 g of fat, respectively. [1-(13)C] palmitate was added in breakfast only. Plasma TAG and chylomicron-TAG (CM-TAG) concentrations and [1-(13)C] palmitate enrichment were sequentially measured. On a consecutive day, an identical breakfast labeled with (123)I-Lipiodol was ingested, followed by a lunch for three controls. (123)I-Lipiodol dynamics was followed in vivo by scintigraphic imaging focused on the stomach, small bowel, and thoracic duct arch. RESULTS: An early rise in plasma and CM-TAG was observed after lunch ingestion. After breakfast, [1-(13)C] palmitate enrichment was maximal 150 and 210 min in plasma TAG and CM-TAG, respectively, decreased thereafter, and increased rapidly (50 min for plasma TAG and 30 min for CM-TAG) after lunch ingestion. Scintigraphic imaging appeared to show that fat ingested at breakfast was retained in part within the gut at lunch time. For the three subjects who ingested a lunch, a decrease of activity in the stomach and small bowel and a tendency for increased activity in the thoracic arch were observed. CONCLUSION: Contribution of fat ingested at breakfast to lipemia after lunch is confirmed. Fat ingested at breakfast was partly retained within the gut and was mobilized after lunch ingestion, as assessed by acceleration of gut emptying and thoracic duct flow after lunch

Use of experimental design methodology for the development of new magnetic siRNA nanovectors (MSN).

International audienceShort interfering RNAs (siRNAs) can downregulate the synthesis of proteins and thus be used to treat certain diseases where the protein synthesis is upregulated, such as cancer. The challenge is to deliver siRNAs in the target cell as they are rapidly degraded by nucleases and have difficulties to cross the cellular membranes. Superparamagnetic iron oxide nanoparticles (SPIONs) are widely studied as platforms for smart biocompatible nanosystems which can be used for magnetic drug targeting and magnetic resonance imaging. The aim of this work was to combine siRNAs, SPIONs, and chitosan, to develop new magnetic siRNA nanovectors suitable for systemic administration. In a first time, the one factor at a time (OFAT) methodology was used to adjust different formulation parameters and to test the feasibility of such a formulation. In a second time, design of experiment (DOE) methodology was used to analyze the influence of these formulation parameters on the physicochemical characteristics hydrodynamic diameter (DH) and ζ-potential. Finally, four MSNs suitable for systemic administration could be identified using the OFAT method. The DOE method showed a significant effect of CR and [NaNO3] on the DH and a significant effect of MR and [siRNA] on the ζ-potential of the nanocarriers