92 research outputs found
On mechanics and monitoring of plunge-roll rotary dressing of grinding wheels
A study is made into the mechanics and monitoring of rotary plunge-roll dressing of grinding wheels using a roll with multi-layer diamonds contained in a hybrid, metal-ceramic bond. A fundamental relationship is obtained between grinding/dressing specific energy and the dressing aggressiveness number Aggrd, revealing a distinct size effect. Results also indicate (i) a nearly linear relationship between grinding and dressing specific energy, and (ii) direct proportionality between dressing specific energy and the acoustic emission (AE) signal. SEM observations indicate that smaller Aggrd produces a grit-dulling phenomenon different from grinding-induced dulling of the grits by attrition, which causes rapid workpiece-material adhesion
cGAL, a temperature-robust GAL4–UAS system for Caenorhabditis elegans
The GAL4–UAS system is a powerful tool for manipulating gene expression, but its application in Caenorhabditis elegans has not been described. Here we systematically optimize the system's three main components to develop a temperature-optimized GAL4–UAS system (cGAL) that robustly controls gene expression in C. elegans from 15 to 25 °C. We demonstrate this system's utility in transcriptional reporter analysis, site-of-action experiments and exogenous transgene expression; and we provide a basic driver and effector toolkit
A Conserved Function of C. elegans CASY-1 Calsyntenin in Associative Learning
BACKGROUND: Whole-genome association studies in humans have enabled the unbiased discovery of new genes associated with human memory performance. However, such studies do not allow for a functional or causal testing of newly identified candidate genes. Since polymorphisms in Calsyntenin 2 (CLSTN2) showed a significant association with episodic memory performance in humans, we tested the C. elegans CLSTN2 ortholog CASY-1 for possible functions in the associative behavior of C. elegans. METHODOLOGY/PRINCIPAL FINDINGS: Using three different associative learning paradigms and functional rescue experiments, we show that CASY-1 plays an important role during associative learning in C. elegans. Furthermore, neuronal expression of human CLSTN2 in C. elegans rescues the learning defects of casy-1 mutants. Finally, genetic interaction studies and neuron-specific expression experiments suggest that CASY-1 may regulate AMPA-like GLR-1 glutamate receptor signaling. CONCLUSION/SIGNIFICANCE: Our experiments demonstrate a remarkable conservation of the molecular function of Calsyntenins between nematodes and humans and point at a role of C. elegans casy-1 in regulating a glutamate receptor signaling pathway
Quantitative Electron Diffraction Evidence for One-Dimensional Ordering in Magnetite above the Verwey Transition
Endocrine correlates of placental permeability during gestation in the pig
Hormone and electrolyte concentrations were determined in amniotic and allantoic fluid collected from sows at 47-112 days of gestation and correlated with placental and fetal membrane electrolyte permeability. Significant differences between the electrolyte and endocrine gestational profiles of amniotic and allantoic fluid were identified. The principal endocrine correlate (r > 0.62; P < 0.01) of gestational changes in the sodium and chloride permeabilities of the porcine placenta and fetal membranes was found to be the concentration of oestrogen in the amniotic fluid
Descriptive Assessment Method to Reduce Overall Disruptive Behavior in a Preschool Classroom
Spousal Age Differences and Sex Differences in Life Expectancy are Confounders of Matrilateral Biases in Kin Investment
Preliminary investigation of the combined effect of heat treatment and incubation temperature on the viability of the probiotic micro-organisms in freshly made yogurt
The combined effects of heat treatment and incubation temperature were studied on the viability and fermentation time of probiotics in ABY 1 probiotic yogurt (Lactobacillus acidophilus, Bifidobacterium spp. and yogurt bacteria). Three levels of heat treatment (85°C for 30 min, 95°C for 5 min and 95°C for 15 min) and three levels of incubation temperature (37, 40 and 44°C) were selected. At the end of fermentation, the maximum viability of probiotics (for both L. acidophilus and Bifidobacterium spp.) was observed when the milk was heated at 95°C for 15 min and incubated at 37°C. Incubation time was only affected by the incubation temperature and, at 37°C, the longest incubation time of about 6 h was needed to achieve the highest viable counts of L. acidophilus and Bifidobacterium spp.Fil: Mortazavian, Amir. Islamic Azad University; IránFil: Ehsani, Mohammadreza. University Of Tehran; IránFil: Mousavi, Mohammad. University Of Tehran; IránFil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Santa Fe. Instituto de LactologĂa Industrial. Universidad Nacional del Litoral. Facultad de IngenierĂa QuĂmica. Instituto de LactologĂa Industrial; ArgentinaFil: Emamdjomeh, Zahra. University Of Tehran; IránFil: Sohrabvandi, Sara. Islamic Azad University; IránFil: Rezaei, Karamatollah. University Of Tehran; Irá
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