6 research outputs found
Rapid identification viruses from nasal pharyngeal aspirates in acute viral respiratory infections by RT-PCR and electrospray ionization mass spectrometry
An Improved Calibration Method for the Matrix-Assisted Laser Desorption/Ionization-Fourier Transform Ion Cyclotron Resononance Analysis of 15
Reversed Phase Liquid Chromatography Hyphenated to Continuous Flow—Extractive Desorption Electrospray Ionization—Mass Spectrometry for Analysis and Charge State Manipulation of Undigested Proteins
Mass Spectrometry
International audienceFor twenty years or so now, mass spectrometry has been used to get exact measurements of the mass of biological molecules such as proteins, nucleic acids,oligosaccharides, and so on. Over the past ten years, this technology has followed the trend toward miniaturisation and the samples required can be much smaller. In particular, the nanoelectrospray source (online or by needle) allow one to work at flow rates of a few tens of nanolitres/min. There are many applications, both in the field of proteomics and in the analysis of protein structure, dynamics, and interactions. Combining this source with nanoHPLC, complex mixtures only available in small quantities can be separated and analysed online. There are also some advantages over conventional HPLC, despite a set of constraints related to the small dimensions and low flow rates. Combining capillary electrophoresis with the electrospray source also gives useful results, with its own set of advantages and constraints. Finally, developments are currently underway to combine this source with chips, providing a means of separation and analysis online