Non-perturbative improvement of the axial current for dynamical Wilson fermions

A non-perturbative determination of the axial current improvement coefficient cA is performed with two flavors of dynamical improved Wilson fermions and plaquette gauge action. The improvement condition is formulated with Schroedinger functional boundary conditions and enforced at constant physical volume. Large sensitivity is obtained by using two different pseudo-scalar states in the PCAC relation. We estimate the resulting correction to F_PS at beta=5.2 to be around 10%.Comment: 13 pages, 5 figures, JHEP styl

Evaluation of Sample Preparation Strategies for Human Milk and Plasma Proteomics

Sample preparation is the most critical step in proteomics as it directly affects the subset of proteins and peptides that can be reliably identified and quantified. Although a variety of efficient and reproducible sample preparation strategies have been developed, their applicability and efficacy depends much on the biological sample. Here, three approaches were evaluated for the human milk and plasma proteomes. Protein extracts were digested either in an ultrafiltration unit (filter-aided sample preparation, FASP) or in-solution (ISD). ISD samples were desalted by solid-phase extraction prior to nRPC-ESI-MS/MS. Additionally, milk and plasma samples were directly digested by FASP without prior protein precipitation. Each strategy provided inherent advantages and disadvantages for milk and plasma. FASP appeared to be the most time efficient procedure with a low miscleavage rate when used for a biological sample aliquot, but quantitation was less reproducible. A prior protein precipitation step improved the quantitation by FASP due to significantly higher peak areas for plasma and a much better reproducibility for milk. Moreover, the miscleavage rate for milk, the identification rate for plasma, and the carbamidomethylation efficiency were improved. In contrast, ISD of both milk and plasma resulted in higher miscleavage rates and is therefore less suitable for targeted proteomics

Comprehensive Profiling of the Native and Modified Peptidomes of Raw Bovine Milk and Processed Milk Products

Bovine milk contains a variety of endogenous peptides, partially formed by milk proteases that may exert diverse bioactive functions. Milk storage allows further protease activities altering the milk peptidome, while processing, e.g., heat treatment can trigger diverse chemical reactions, such as Maillard reactions and oxidations, leading to different posttranslational modifications (PTMs). The influence of processing on the native and modified peptidome was studied by analyzing peptides extracted from raw milk (RM), ultra-high temperature (UHT) milk, and powdered infant formula (IF) by nano reversed-phase liquid chromatography coupled online to electrospray ionization (ESI) tandem mass spectrometry. Only unmodified peptides proposed by two independent software tools were considered as identified. Thus, 801 identified peptides mainly originated from αS- and β-caseins, but also from milk fat globular membrane proteins, such as glycosylation-dependent cell adhesion molecule 1. RM and UHT milk showed comparable unmodified peptide profiles, whereas IF differed mainly due to a higher number of β-casein peptides. When 26 non-enzymatic posttranslational modifications (PTMs) were targeted in the milk peptidomes, 175 modified peptides were identified, i.e., mostly lactosylated and a few hexosylated or oxidized peptides. Most modified peptides originated from αS-caseins. The numbers of lactosylated peptides increased with harsher processing

Non-perturbative quark mass renormalization in two-flavor QCD

The running of renormalized quark masses is computed in lattice QCD with two flavors of massless O(a) improved Wilson quarks. The regularization and flavor independent factor that relates running quark masses to the renormalization group invariant ones is evaluated in the Schroedinger Functional scheme. Using existing data for the scale r_0 and the pseudoscalar meson masses, we define a reference quark mass in QCD with two degenerate quark flavors. We then compute the renormalization group invariant reference quark mass at three different lattice spacings. Our estimate for the continuum value is converted to the strange quark mass with the help of chiral perturbation theory.Comment: 25 pages, 6 figures; sections 1 and 4 rearranged, minor change to the summary plo

SchülerInnen und Studierende lernen gemeinsam mehr?!: Praxis von Anfang an ; Chemieunterricht differenzierend gestaltet durch Chemielehramtsstudierende der ersten Semester

Standards der Lehrerbildung fordern neben Möglichkeiten der eigenen Erprobung in der Pra-xis auch eine intensive Behandlung des Themas Individualisierung im Lehramtsstudium. Be-gründet sind diese Forderungen durch die zunehmende Heterogenität der Lernenden und die häufig von Studierenden bemängelte unzureichende Praxisorientierung ihres Studiums. Das in dieser Studie untersuchte Konzept der „Praxis von Anfang an“ ermöglicht einen ersten Ein-blick in die Berufsrealität der Lehrperson bereits im ersten Semester, wobei ein Schwerpunkt der Unterrichtsgestaltung in der Differenzierung von Chemieunterricht liegt. Ziel der Studie ist es, die Wirksamkeit dieses frühen Schulpraktikums zu bewerten sowie die Überzeugungen der Schülerinnen und Schüler zu offenem, differenzierendem Chemieunter-richt durch Studierende zu erforschen. In drei Erprobungen wurden daher Pre-Post-Befragungen der Studierenden, Schülerleistungstests, Schülerbefragungen, Unterrichtsdiag-nostik und Inhaltsanalysen durchgeführt. Die Arbeit zeichnet sich durch diesen vielfältigen Einsatz von Forschungsmethoden aus und konnte somit auf explorative Weise erste Erkennt-nisse zu frühen Praxiserfahrungen generieren

Non-perturbative renormalization of the axial current with dynamical Wilson fermions

We present a new normalization condition for the axial current, derived from the PCAC relation with non-vanishing quark mass. This condition is expected to reduce mass effects in the chiral extrapolation of the results for the normalization factor Z_A. The application to the two-flavor theory with improved Wilson fermions shows that this expectation is indeed fulfilled. Using the Schroedinger functional setup we calculate Z_A(g_0^2) as well as the vector current normalization factor Z_V(g_0^2) for beta = 6/g_0^2 >= 5.2.Comment: 15 pages, 4 figures, 2 tables, JHEP styl

Profiling of Low-Molecular-Weight Carbonyls and Protein Modifications in Flavored Milk

Thermal treatments of dairy products favor oxidations, Maillard reactions, and the formation of sugar or lipid oxidation products. Additives including flavorings might enhance these reactions or even induce further reactions. Here we aimed to characterize protein modifications in four flavored milk drinks using samples along the production chain—raw milk, pasteurization, mixing with flavorings, heat treatment, and the commercial product. Therefore, milk samples were analyzed using a bottom up proteomics approach and a combination of data-independent (MSE) and data-dependent acquisition methods (DDA). Twenty-one small carbonylated lipids were identified by shotgun lipidomics triggering 13 protein modifications. Additionally, two Amadori products, 12 advanced glycation end products (AGEs), and 12 oxidation-related modifications were targeted at the protein level. The most common modifications were lactosylation, formylation, and carboxymethylation. The numbers and distribution of modification sites present in raw milk remained stable after pasteurization and mixing with flavorings, while the final heat treatment significantly increased lactosylation and hexosylation in qualitative and quantitative terms. The processing steps did not significantly affect the numbers of AGE-modified, oxidized/carbonylated, and lipid-carbonylated sites in proteins

Analysis of the Endogenous Peptidomes of Different Infant Formula Types and Human Milk

Infant formula (IF) is a commonly used replacement whenever mother’s own milk is not available. Most IFs are based on cow milk (powders, liquids). Alternatives, based on other sources such as goat milk or plants, exist. Independent of the source, IF production and composition are strictly regulated. Besides proteins, minerals, and lipids, milk contains a variety of endogenous peptides. Whereas the human milk peptidome has been studied intensively, the peptidomes of IFs have been mostly neglected. This study investigated the peptidomes of different types of first stage IF, including cow milk-based powders and liquids, and powdered goat milk-based IF, highlighting major similarities and differences to human milk. Extracted native peptidomes were analyzed by nanoRPC-ESI-MS/MS using two different fragmentation techniques allowing the confident identification of 1587 peptides. β-Casein peptides dominated in all samples. Interestingly, powdered and liquid cow milk-based IFs differed in the numbers of β- and αS1-casein peptides, indicating processing-derived variations. However, the peptidomes of cow and goat milk-based IF appeared to be more comparable to each other than to human milk. Despite an overlap in the major source proteins, many peptide sequences were different, i.e., species-specific. Remarkably, the data indicate that the human milk peptidome might be donor-specific as well