Synergistic co-cultivation of activated sludge and microalgae in enhancing lipid production and N-laden wastewater treatment

The influence of inoculation ratios of activated sludge and microalgae were investigated in this study in the aspects of biomass yield, lipid yield and total nitrogen (TN) removal efficiency. It was observed that mixed culture of activated sludge/microalgae with the ratio 1:1 and 1:0.75 achieved a maximum lipid production up to 0.144 g/L and 0.133 g/L as compared with microalgae culture alone, which was only 0.081 g/L. The highest total nitrogen (TN) removal was observed with 1:1 and 1:0.75 ratios of activated sludge/microalgae cultures ranging from 96.3-96.9% removal efficiency, which was an improvement of about 90% removal efficiency compared to the activated sludge culture (6.25±0.08%). The flocculation efficiency was generally improved in mixed cultures of activated sludge andmicroalgae in comparison with only activated sludge culture and microalgae culture alone.Keywords: activated sludge; microalgae; co-cultivation; lipid; nitrogen removal

Optimization of Growth Medium for Efficient Cultivation of Lactobacillus salivarius i 24 using Response Surface Method

Production of Lactobacillus salivarius i 24, a probiotic strain for chicken, was studied in batch fermentation using 500 mL Erlenmeyer flask. Response surface method (RSM) was used to optimize the medium for efficient cultivation of the bacterium. The factors investigated were yeast extract, glucose and initial culture pH. A polynomial regression model with cubic and quartic terms was used for the analysis of the experimental data. Estimated optimal conditions of the factors for growth of L. salivarius i 24 were; 3.32 % (w/v) glucose, 4.31 % (w/v) yeast extract and initial culture pH of 6.10

Submillimeter Array multiline observations of the massive star-forming region IRAS 18089-1732

Submillimeter Array (SMA) observations of the high-mass star-forming region IRAS 18089-1732 in the 1 mm and 850 $\mu$m band with 1 GHz bandwidth reveal a wealth of information. We present the observations of 34 lines from 16 different molecular species. Most molecular line maps show significant contributions from the outflow, and only few molecules are confined to the inner core. We present and discuss the molecular line observations and outline the unique capabilities of the SMA for future imaging line surveys at high spatial resolution.Comment: Accepted for ApJ Letters, SMA special volum

SMA outflow/disk studies in the massive star-forming region IRAS18089-1732

SMA observations of the massive star-forming region IRAS 18089-1732 in the 1mm and 850mu band reveal outflow and disk signatures in different molecular lines. The SiO(5--4) data show a collimated outflow in the northern direction. In contrast, the HCOOCH3(20--19) line, which traces high-density gas, is confined to the very center of the region and shows a velocity gradient across the core. The HCOOCH3 velocity gradient is not exactly perpendicular to the outflow axis but between an assumed disk plane and the outflow axis. We interpret these HCOOCH3 features as originating from a rotating disk that is influenced by the outflow and infall. Based on the (sub-)mm continuum emission, the mass of the central core is estimated to be around 38M_sun. The dynamical mass derived from the HCOOCH3 data is 22Msun, of about the same order as the core mass. Thus, the mass of the protostar/disk/envelope system is dominated by its disk and envelope. The two frequency continuum data of the core indicate a low dust opacity index beta ~ 1.2 in the outer part, decreasing to beta ~ 0.5 on shorter spatial scales.Comment: 7 pages of text, 1 table, 3 figures, accepted for ApJ Letter

Studies of Wilms’ Tumor (WT1) Gene Expression in Adult Acute Leukemias in Singapore

Biomarkers provide certain values for diagnosis, monitor treatment efficacy, or for the development of novel therapeutic approach for particular diseases. Thus, the identification of specific of biomarkers for specific medical problems, including malignant diseases may be valuable in medical practice. In the study, we have used the Wilms’ tumor gene (WT1) as a biomarker to evaluate its expression in local adult patients with newly diagnosed acute leukemia, including both acute myeloid and lymphoid leukemias (AML and ALL)

Development of a novel cell-based assay system EPISSAY for screening epigenetic drugs and liposome formulated decitabine

Extent: 11 p.BACKGROUND: Despite the potential of improving the delivery of epigenetic drugs, the subsequent assessment of changes in their epigenetic activity is largely dependent on the availability of a suitable and rapid screening bioassay. Here, we describe a cell-based assay system for screening gene reactivation. METHODS: A cell-based assay system (EPISSAY) was designed based on a silenced triple-mutated bacterial nitroreductase TMnfsB fused with Red-Fluorescent Protein (RFP) expressed in the non-malignant human breast cell line MCF10A. EPISSAY was validated using the target gene TXNIP, which has previously been shown to respond to epigenetic drugs. The potency of a epigenetic drug model, decitabine, formulated with PEGylated liposomes was also validated using this assay system. RESULTS: Following treatment with DNA methyltransferase (DNMT) and histone deacetylase (HDAC) inhibitors such as decitabine and vorinostat, increases in RFP expression were observed, indicating expression of RFP-TMnfsB. The EPISSAY system was then used to test the potency of decitabine, before and after PEGylated liposomal encapsulation. We observed a 50% higher potency of decitabine when encapsulated in PEGylated liposomes, which is likely to be due to its protection from rapid degradation. CONCLUSIONS: The EPISSAY bioassay system provides a novel and rapid system to compare the efficiencies of existing and newly formulated drugs that reactivate gene expression.Sue Ping Lim, Raman Kumar, Yamini Akkamsetty, Wen Wang, Kristen Ho, Paul M. Neilsen, Diego J. Walther, Rachel J. Suetani, Clive Prestidge and David F. Calle

Prevalence of Tidal Interactions among Local Seyfert Galaxies: The Control Experiment

We test whether there is a relation between the observed tidal interactions and Seyfert activity by imaging in HI twenty inactive galaxies at the same spatial resolution and detection threshold as the Seyfert sample. This control sample of inactive galaxies were closely matched in Hubble type, range in size and inclination, and have roughly comparable galaxy optical luminosity to the Seyfert galaxies. We find that only ~15% of the galaxies in our control sample are disturbed in HI, whereas the remaining ~85% show no disturbances whatsoever in HI. Even at a spatial resolution of ~10 kpc, none of the latter galaxies show appreciable HI disturbances reminiscent of tidal features. In a companion paper (Kuo et al. 2008), we report results from the first systematic imaging survey of Seyfert galaxies in atomic hydrogen (HI) gas. We find that only ~28% of the eighteen Seyfert galaxies in that sample are visibly disturbed in optical starlight. By contrast, ~94% of the same Seyfert galaxies are disturbed spatially and usually also kinematically in HI gas on galactic scales of >~20 kpc. In at least ~67% and up to perhaps ~94% of cases, the observed disturbances can be traced to tidal interactions with neighboring galaxies detected also in HI. The dramatic contrast between the observed prevalence of HI disturbances in the Seyfert and control samples implicates tidal interactions in initiating events that lead to luminous Seyfert activity in a large fraction of local disk galaxies.Comment: 38 pages, 27 figures, Accepted for publication in ApJ. Changes from previous version: The title of the previous version of this paper appeared in Astroph was incorrect. It has been replaced with the correct on

Search for Calibrators for the Submillimeter Array: I. High-Mass Star Forming Regions

We present initial results of an ongoing search for interferometric calibrators at submillimeter (sub-mm) wavelengths with the Submillimeter Array (SMA). Powerful radio galaxies are commonly used as calibrators at centimeter and millimeter wavelengths, but many are not strong enough to serve as calibrators at sub-mm wavelengths because of their rapidly declining flux densities toward shorter wavelengths. The inability to find a calibrator close to the target source may limit or even prevent us from imaging many interesting sources at sub-mm wavelengths. Here, we investigate whether high-mass protostellar objects and ultracompact HII regions can serve as useful calibrators for the SMA. The dust emission associated with these objects makes them among the brightest sub-mm sources in the sky. Our observations at 0.85 mm (345 GHz) with an angular resolution of ~3" reveal that although a large fraction of the dust emission originates from an extended ``halo'' component, a compact unresolved component often remains that when sufficiently strong may serve as a useful calibrator. These observations also provide a first glimpse at the small-scale distribution of dust around ultracompact HII regions and high-mass protostellar objects at sub-mm wavelengths. We discuss the origin of the core-halo structure seen in many sources, and conclude with suggestions for future searches for calibrators with the SMA.Comment: 3 figures, accepted for publication in ApJ Letter

Mapping the Outflow from G5.89-0.39 in SiO(5-4)

We have mapped the ultracompact HII region, G5.89-0.39, and its molecular surroundings with the Submillimeter Array at 2".8 x 1".8 angular resolution in 1.3 mm continuum, SiO(5-4), and eight other molecular lines. We have resolved for the first time the highly energetic molecular outflow in this region. At this resolution, the outflow is definitely bipolar and appears to originate in a 1.3 mm continuum source. The continuum source peaks in the center of the HII region. The axis of the outflow lines up with a recently discovered O5V star.Comment: 3 Figures, Accepted for publication in ApJ Letter