Improving the reliability of warehouse operations in the 3PL Industry: an Australian 3PL case study.

Warehouses play an important value-adding role to provide a competitive edge, support business growth and efficiency throughout their supply chains. This paper explores warehouse operations (i.e. receiving, put away, picking, packing and shipping) and a series of challenges faced in managing warehouse operations in general and the 3PL industry in particular. Especially, the paper analyses the warehouse and logistics operations of an Australian 3PL company. By utilizing a mixed method approach both qualitative and quantitative data was gathered through in-depth interviews, direct observations and warehouse operations records. The collected data were then analyzed using thematic analysis. The data was screened and coded to further develop major themes to identify the problematic areas. This analysis helped to identify issues in operations related to the receiving process, missing, picking and locating items as well as human errors. While multiple approaches could be utilized to improve the operations, there yet remained a major challenge to manage operations within the planned budgetary limits. This research, therefore, provides some solutions/recommendations to improve the case company’s warehouse operations through staff empowerment, management process improvement, order dispatch and return process improvement, improvement in record keeping, and recruiting more personnel. These recommendations ensure company’s efficiency while balancing its challenges both in operations and budgetary constraints. This study thus provides an evidence to improve the reliability of warehouse operations through systematic process improvement.N/

A study of consumer behavior and willingness to pay towards cosmetic products of generation Z in Hochiminh city

The global cosmetics industry is experiencing robust growth and Generation Z (Gen Z) is a potential customer source of this market. This research examines customers’ intentions to purchase cosmetic goods of Gen Z in Hochiminh city using perceived value factors as the antecedents of attitude in the model. A cross-sectional study, applying a convenient sampling method, was conducted to collect data from Gen Z people in January 2023. There were no specific cosmetics products included in the study. Descriptive analysis and Partial Least Square (PLS) method of Structural Equation Modeling (SEM) with SmartPLS 4.0.8.7 software were used to analyze the research data. The PLS-SEM analysis of 723 responses showed that attitudes toward purchasing cosmetic products are significantly positively impacted by perceived environmental value. A more positive attitude would result from increased brand credibility and product understanding of cosmetics. There was no evidence to support the impact of other perceived values (specifically, health, safety, social, spiritual, and ethical) on attitudes toward consumer behavior. Attitude was important in predicting willingness to pay (both direct and indirect). This study helps industry professionals to advance the qualities of cosmetic products by increasing and improving environmental value, product knowledge, and brand credibility. The eco-friendly pattern of production and marketing strategies focusing on product ingredients, manufacturing processes, and quality standards are necessary to enhance brand credibility and product understanding, align with consumer preferences and achieve greater success in the market

Mitochondrial physiology

As the knowledge base and importance of mitochondrial physiology to evolution, health and disease expands, the necessity for harmonizing the terminology concerning mitochondrial respiratory states and rates has become increasingly apparent. The chemiosmotic theory establishes the mechanism of energy transformation and coupling in oxidative phosphorylation. The unifying concept of the protonmotive force provides the framework for developing a consistent theoretical foundation of mitochondrial physiology and bioenergetics. We follow the latest SI guidelines and those of the International Union of Pure and Applied Chemistry (IUPAC) on terminology in physical chemistry, extended by considerations of open systems and thermodynamics of irreversible processes. The concept-driven constructive terminology incorporates the meaning of each quantity and aligns concepts and symbols with the nomenclature of classical bioenergetics. We endeavour to provide a balanced view of mitochondrial respiratory control and a critical discussion on reporting data of mitochondrial respiration in terms of metabolic flows and fluxes. Uniform standards for evaluation of respiratory states and rates will ultimately contribute to reproducibility between laboratories and thus support the development of data repositories of mitochondrial respiratory function in species, tissues, and cells. Clarity of concept and consistency of nomenclature facilitate effective transdisciplinary communication, education, and ultimately further discovery

Mitochondrial physiology

As the knowledge base and importance of mitochondrial physiology to evolution, health and disease expands, the necessity for harmonizing the terminology concerning mitochondrial respiratory states and rates has become increasingly apparent. The chemiosmotic theory establishes the mechanism of energy transformation and coupling in oxidative phosphorylation. The unifying concept of the protonmotive force provides the framework for developing a consistent theoretical foundation of mitochondrial physiology and bioenergetics. We follow the latest SI guidelines and those of the International Union of Pure and Applied Chemistry (IUPAC) on terminology in physical chemistry, extended by considerations of open systems and thermodynamics of irreversible processes. The concept-driven constructive terminology incorporates the meaning of each quantity and aligns concepts and symbols with the nomenclature of classical bioenergetics. We endeavour to provide a balanced view of mitochondrial respiratory control and a critical discussion on reporting data of mitochondrial respiration in terms of metabolic flows and fluxes. Uniform standards for evaluation of respiratory states and rates will ultimately contribute to reproducibility between laboratories and thus support the development of data repositories of mitochondrial respiratory function in species, tissues, and cells. Clarity of concept and consistency of nomenclature facilitate effective transdisciplinary communication, education, and ultimately further discovery

The role of c14orf2 protein in structure and function of mammalian ATP synthase

The F1Fo-ATP synthase (EC 3.6.3.14) is a key enzyme of the mitochondrial oxidative phosphorylation system (OXPHOS) - using the proton gradient generated by the respiratory chain it synthetizes approximately 90 % of cellular ATP. The subunit arrangement of its Fo domain has not been yet described in detail. At present, the research on ATP synthase research is focused mostly on revealing the structure of the proton channel a so that it is possible to precisely define the molecular mechanism of the ATP synthase rotation generation. The role of the supernumery subunits of Fo domain represents another unresolved issue. These proteins specific for eukaryotic ATP synthases are not essential for synthetic activity, instead they are putatively involved in assembly or stabilization of the enzyme complex. One of such subunits is the nuclear encoded MLQ protein (or also 6.8 kDa proteolipid or MP68), which is conserved only in vertebrates. The aim of this diploma thesis was to reveal the role of this subunit in the structure, assembly and function of the F1Fo-ATP synthase. For these purposes, cellular model of the HEK293 line with the deficiency of the MLQ protein was established employing the CRISPR/Cas9 method with paired nickases (the knock-out MLQ, MLQ KO) as part of the thesis. Three chosen MLQ KO lines..

New components and functions of mitochondrial ATP synthase.

The system of oxidative phosphorylation, or respiratory chain in mitochondria gives the eukaryotic cell total majority of the energy it receives and uses in the form of ATP. F1Fo-ATP synthase, powered by the proton-motive force is directly responsible for the ATP synthesis. Diseases connected to the ATP synthesis can have even lethal consequences. There is therefore no doubt about the need for a detailed analysis of the structure of this enzyme. What is left is to reveal the structure of the transmembrane domains, which are not involved in the synthesis itself, but they can for example work as stabilisers or assembly factors. Outside the synthesis activity the dimers of F1Fo-ATP synthase are apparently taking part in the formation of the cristae of the inner membrane of a mitochondrion. Recently, the role of the enzyme is also considered in the creation of the mitochondrial permeability transition pore

The role of c14orf2 protein in structure and function of mammalian ATP synthase

The F1Fo-ATP synthase (EC 3.6.3.14) is a key enzyme of the mitochondrial oxidative phosphorylation system (OXPHOS) - using the proton gradient generated by the respiratory chain it synthetizes approximately 90 % of cellular ATP. The subunit arrangement of its Fo domain has not been yet described in detail. At present, the research on ATP synthase research is focused mostly on revealing the structure of the proton channel a so that it is possible to precisely define the molecular mechanism of the ATP synthase rotation generation. The role of the supernumery subunits of Fo domain represents another unresolved issue. These proteins specific for eukaryotic ATP synthases are not essential for synthetic activity, instead they are putatively involved in assembly or stabilization of the enzyme complex. One of such subunits is the nuclear encoded MLQ protein (or also 6.8 kDa proteolipid or MP68), which is conserved only in vertebrates. The aim of this diploma thesis was to reveal the role of this subunit in the structure, assembly and function of the F1Fo-ATP synthase. For these purposes, cellular model of the HEK293 line with the deficiency of the MLQ protein was established employing the CRISPR/Cas9 method with paired nickases (the knock-out MLQ, MLQ KO) as part of the thesis. Three chosen MLQ KO lines..

The role of c14orf2 protein in structure and function of mammalian ATP synthase

F1Fo-ATP syntáza (EC 3.6.3.14) je klíčovým enzymem v systému mitochondriální oxidativní fosforylace (OXPHOS) - využívá protonový gradient vybudovaný respiračním řetězcem k syntéze přibližně 90 % buněčného ATP. Uspořádání podjednotek membránové domény Fo tohoto enzymu ale ještě není stále detailně popsáno. Výzkum ATP syntázy je v současnosti zaměřen především na odhalení struktury protonového kanálu a, aby bylo možno přesně definovat molekulární mechanismus generování rotace ATP syntázy. Další nevyřešenou otázkou představuje úloha tzv. nadpočetných podjednotek Fo domény. Tyto proteiny specifické pro eukaryotní ATP syntázy nejsou esenciální pro syntázovou aktivitu a předpokládá se, že se mohou podílet na sestavování a stabilizaci enzymového komplexu. Jednou z těchto podjednotek je jaderně kódovaný protein MLQ (nebo také 6,8 kDa proteolipid či MP68), který je konzervován pouze u obratlovců. Cílem této diplomové práce bylo zjistit, jakou úlohu má tato podjednotka ve struktuře, asemblaci a funkci F1Fo-ATP syntázy. Pro tyto účely byl v rámci diplomové práce vytvořen buněčný model z linie HEK293 s deficiencí proteinu MLQ metodou CRISPR/Cas9 s párovanými nikázami (tzv. knock-out MLQ, MLQ KO). Tři vybrané MLQ KO linie byly podrobeny elektroforetickým analýzám struktury enzymu (SDS-PAGE, BN-PAGE a CN-PAGE) i...The F1Fo-ATP synthase (EC 3.6.3.14) is a key enzyme of the mitochondrial oxidative phosphorylation system (OXPHOS) - using the proton gradient generated by the respiratory chain it synthetizes approximately 90 % of cellular ATP. The subunit arrangement of its Fo domain has not been yet described in detail. At present, the research on ATP synthase research is focused mostly on revealing the structure of the proton channel a so that it is possible to precisely define the molecular mechanism of the ATP synthase rotation generation. The role of the supernumery subunits of Fo domain represents another unresolved issue. These proteins specific for eukaryotic ATP synthases are not essential for synthetic activity, instead they are putatively involved in assembly or stabilization of the enzyme complex. One of such subunits is the nuclear encoded MLQ protein (or also 6.8 kDa proteolipid or MP68), which is conserved only in vertebrates. The aim of this diploma thesis was to reveal the role of this subunit in the structure, assembly and function of the F1Fo-ATP synthase. For these purposes, cellular model of the HEK293 line with the deficiency of the MLQ protein was established employing the CRISPR/Cas9 method with paired nickases (the knock-out MLQ, MLQ KO) as part of the thesis. Three chosen MLQ KO lines...Katedra buněčné biologieDepartment of Cell BiologyPřírodovědecká fakultaFaculty of Scienc

A study of consumer behavior and willingness to pay towards cosmetic products of generation Z in Hochiminh city

The global cosmetics industry is experiencing robust growth and Generation Z (Gen Z) is a potential customer source of this market. This research examines customers’ intentions to purchase cosmetic goods of Gen Z in Hochiminh city using perceived value factors as the antecedents of attitude in the model. A cross-sectional study, applying a convenient sampling method, was conducted to collect data from Gen Z people in January 2023. There were no specific cosmetics products included in the study. Descriptive analysis and Partial Least Square (PLS) method of Structural Equation Modeling (SEM) with SmartPLS 4.0.8.7 software were used to analyze the research data. The PLS-SEM analysis of 723 responses showed that attitudes toward purchasing cosmetic products are significantly positively impacted by perceived environmental value. A more positive attitude would result from increased brand credibility and product understanding of cosmetics. There was no evidence to support the impact of other perceived values (specifically, health, safety, social, spiritual, and ethical) on attitudes toward consumer behavior. Attitude was important in predicting willingness to pay (both direct and indirect). This study helps industry professionals to advance the qualities of cosmetic products by increasing and improving environmental value, product knowledge, and brand credibility. The eco-friendly pattern of production and marketing strategies focusing on product ingredients, manufacturing processes, and quality standards are necessary to enhance brand credibility and product understanding, align with consumer preferences and achieve greater success in the market

ĐỊNH DANH VÀ NHÂN GIỐNG GỪNG ĐEN (Distichochlamys citrea) BẰNG PHƯƠNG PHÁP GIÂM HOM

Distichochlamys citrea is a medicinal plant endemic to Vietnam and has valuable benefits for treating a variety of diseases. The black ginger samples collected from Bach Ma National Park have differences in colour and shape of stems and leaves. These two characteristics are essential for identifying the correct D. citrea species. We determined the morphological and molecular characteristics of the D. citrea samples and evaluated the effect of factors on the propagation of these species with the rhizome cutting method. Using the ITS marker, we recognised that these black ginger samples belong to Distichochlamys citrea species. The 2 g rhizome is suitable for cutting. Trichoderma (0.2 kg/kg rhizome) was utilized for treating the cutting rhizome. The substrate containing coir, sand, clean soil, earthworm manure, and rice husks (the ratio of 1:1:1:1:1) is the best for the growth of the cuttings. A 100 ppm IBA solution is the best for the growth and rooting of cutting plantlets. After nine months of growing, we reached the following results: 3.2 shoots/cutting rhizome, 9.6 roots/cutting rhizome, root length of 21.29 cm, plant height of 25.75 cm, and 3.58 leaves/plant.Distichochlamys citrea là loại dược liệu đặc hữu của Việt Nam và có tác dụng quý trong điều trị nhiều loại bệnh. Các mẫu gừng đen thu thập từ Vườn Quốc gia Bạch Mã có sự khác biệt về màu sắc và dạng thân, lá, nên việc xác định chính xác loài dựa vào hai đặc điểm này là cần thiết để thu đúng loài D. citrea. Chúng tôi đã xác định đặc điểm hình thái và phân tử của các mẫu gừng đen và đánh giá ảnh hưởng của các nhân tố đến nhân giống cây D. citrea bằng phương pháp giâm hom. Với chỉ thị ITS, chúng tôi nhận thấy các mẫu gừng này thuộc loài Distichochlamys citrea. Củ có khối lượng 2 g là thích hợp cho giâm hom. Việc sử dụng Trichoderma (0,2 kg/kg hom củ) cho xử lý hom củ và giâm trên giá thể gồm xơ dừa, cát, đất sạch, phân giun quế và trấu hun (tỷ lệ 1:1:1:1:1) có ảnh hưởng tốt nhất đến sinh trưởng của cây gừng đen. Dung dịch tưới IBA 100 ppm là phù hợp cho sinh trưởng và tạo rễ của các cây giâm hom. Sau chín tháng giâm củ, chúng tôi đạt được kết quả sau: 3,2 chồi/hom, 9,6 rễ/hom, rễ dài 21,29 cm, cây cao 25,75 cm và 3,58 lá/cây