Short day length-induced decrease of cesium uptake without altering potassium uptake manner in poplar

Short day length-induced alteration of potassium (K) localization in perennial trees is believed to be a mechanism for surviving and adapting to severe winters. To investigate the relationship between cesium (Cs) and K localizations, a model tree poplar, hybrid aspen T89, was employed. Under short day length conditions, the amount of 137Cs absorbed through the root and translocated to the root was drastically reduced, but 42K was not. Potassium uptake from the rhizosphere is mediated mainly by KUP/HAK/KT and CNGC transporters. In poplar, however, these genes were constantly expressed under short-day conditions except for a slight increase in the expression a KUP/HAK/KT gene six weeks after the onset of the short-day treatment. These results indicated that the suppression of 137Cs uptake was triggered by short day length but not regulated by competitive Cs+ and K+ transport. We hypothesize that there are separately regulated Cs+ and K+ transport systems in poplar

CDK19-related disorder results from both loss-of-function and gain-of-function de novo missense variants

Purpose To expand the recent description of a new neurodevelopmental syndrome related to alterations in CDK19. Methods Individuals were identified through international collaboration. Functional studies included autophosphorylation assays for CDK19 Gly28Arg and Tyr32His variants and in vivo zebrafish assays of the CDK19(G28R) and CDK19(Y32H). Results We describe 11 unrelated individuals (age range: 9 months to 14 years) with de novo missense variants mapped to the kinase domain of CDK19, including two recurrent changes at residues Tyr32 and Gly28. In vitro autophosphorylation and substrate phosphorylation assays revealed that kinase activity of protein was lower for p.Gly28Arg and higher for p.Tyr32His substitutions compared with that of the wild-type protein. Injection of CDK19 messenger RNA (mRNA) with either the Tyr32His or the Gly28Arg variants using in vivo zebrafish model significantly increased fraction of embryos with morphological abnormalities. Overall, the phenotype of the now 14 individuals with CDK19-related disorder includes universal developmental delay and facial dysmorphism, hypotonia (79%), seizures (64%), ophthalmologic anomalies (64%), and autism/autistic traits (56%). Conclusion CDK19 de novo missense variants are responsible for a novel neurodevelopmental disorder. Both kinase assay and zebrafish experiments showed that the pathogenetic mechanism may be more diverse than previously thought.Peer reviewe

The Super-ego in \u22Sensei and his will\u22 from \u22KOKORO\u22 Sensei and a death instinct

This essay deals with \u22Sensei and his will\u22 in Natsume Soseki\u27s work \u22KOKORO\u22 What I try to analyze here is Sensei\u27s intrapersonal psychology, in other words, the relation between Sensei\u27s Super-ego and ego. The reason for this approach is because the transformation of Sensei\u27s \u22nature\u22 into \u22Fukashigina osoroshii chikara\u22 or \u22strange and terrible force\u22 after his friend K commits suicide reminds me of Freud\u27s theory of super-ego development. However, it is not my intention to draw a moral conclusion about egoism and punishment. I want to consider this \u22Fukashigina osoroshii chikara\u22 of the super-ego as a death instinct, diverted towards the external world and turned against the self. And through this attempt, I also deal with Soseki\u27s \u22nature\u22

Self identity in \u22Kofu\u22 by Soseki

海老井英次教授退官記念号〈Special Issue〉dedicated to Professor EBII EijiThe narrator in \u22Kofu\u22 (The Miner) by Soseki Natsume maintains that the \u22unity\u22 and \u22coherence\u22 of \u22personality\u22 portrayed in \u22novels\u22 does not exist in reality; moreover, since there is no plot to events in real life, it is impossible to describe them in the form of a novel. He questions the \u22unity\u22 and \u22coherence\u22 or \u22logical form\u22 of languages, questioning personal identity and causality of plot. As a result, he comes to think that novels should depict \u22self\u22 and \u22events\u22 as they are, even though both are incoherent and full of contradictions. However, since narration itself is a linguistic activity, a certain \u22unity\u22 and \u22coherence\u22 or \u22logical form\u22 is inevitable in narrating the incoherence and contradictions. This essay considers whether \u22Kofu\u22 is itself free from such a contradiction or not

A case of severe pneumonia with viremia caused by adenovirus B7 identified by off-label use of a multiplex PCR system

Severe infection with human adenovirus (HAdV) is uncommon in adults, and the lack of reliable point-of-care testing makes the diagnosis challenging. A 39-year-old immunocompetent Indian man developed severe pneumonia, and his condition became life-threatening despite antimicrobial therapy. While sputum and blood cultures remained negative, a multiplex PCR respiratory panel (Filmarray Respiratory Panel), which is only approved for use with nasopharyngeal samples, detected HAdV in the serum and tracheal aspirates on day 5. We therefore initiated ganciclovir, steroids, and intravenous immunoglobulin. The patient’s respiratory condition improved significantly, and he eventually recovered without complications. We later confirmed that conventional PCR of serum detected HAdV-B7. Our case illustrated that a respiratory panel using multiplex PCR successfully detected HAdV in unapproved samples. Such off-label analyses may support the early diagnosis of infections caused by pathogens that are difficult to identify by routine microbiological examination