161 research outputs found
サンプリング方式と尤度関数 (統計数理研究所 研究活動 (平成5年度 研究報告会要旨))
創立50周年記念号(2
The determinant factors for model resolutions obtained using CryoEM method
The CryoEM single particle imaging method has recently received broad
attention in the field of structural biology for determining the structures of
biological molecules. The structures can be resolved to near-atomic resolutions
after rending a large number of CryoEM images measuring molecules in different
orientations. However, the factors for model resolution need to be further
explored. Here, we provide a theoretical framework in conjunction with
numerical simulations to gauge the influence of several key factors that are
determinant in model resolution. We found that the number of measured
projection images and the quality of each measurement (quantified using average
signal-noise-ratio) can be combined to a single factor, which is dominant to
the constructed model resolution. Furthermore, the intrinsic thermal motion of
the molecules and the defocus levels of the electron microscope both have
significant effects on the model resolution. These effects can be
quantitatively summarized using an analytical formula that provides a
theoretical guideline on structure resolutions for given experimental
measurements
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