Aromatic Components of Several Table Grapes Produced in Japan

生食用の雑種4品種('キャンベル・アーリー','巨峰','竜宝','マスカット・ベーリーA')とV.viniferaの'ネオマスカッﾄ'の香気成分を調査した.また,果汁中の揮発性成分をLiquid-liquid extraction法,Solvent extraction法,Head-space analysis法の3種類の方法で抽出し,その結果を比較検討した. 'ネオマスカット'からはV.viniferaの代表的な香気成分であるリナール,α-テルピネオール,シトロネロール,ネロール,ゲラニオールが検出された.とくにゲラニオールが量的に多かった.雑種4品種からは多量の2-フェニルエチルアルコールが検出された.しかしV.labruscaに特徴的に含まれるとされるアントラニル酸メチルは検出されなかった. Liquid-liquid extraction法, Solvent extraction法の2つの抽出方法では,検出成分の種類は変わらなかったが,Solvent extraction法の方が揮発性成分が量的に多く検出された. Head-space analysis 法では得られた揮発性成分が少なく固定もできなかった

Isolation and Characterization of Renal Erythropoietin-Producing Cells from Genetically Produced Anemia Mice

Understanding the nature of renal erythropoietin-producing cells (REPs) remains a central challenge for elucidating the mechanisms involved in hypoxia and/or anemia-induced erythropoietin (Epo) production in adult mammals. Previous studies have shown that REPs are renal peritubular cells, but further details are lacking. Here, we describe an approach to isolate and characterize REPs. We bred mice bearing an Epo gene allele to which green fluorescent protein (GFP) reporter cDNA was knocked-in (EpoGFP) with mice bearing an Epo gene allele lacking the 3′ enhancer (EpoΔ3′E). Mice harboring the mutant EpoGFP/Δ3′E gene exhibited anemia (average Hematocrit 18% at 4 to 6 days after birth), and this perinatal anemia enabled us to identify and purify REPs based on GFP expression from the kidney. Light and confocal microscopy revealed that GFP immunostaining was confined to fibroblastic cells that reside in the peritubular interstitial space, confirming our previous observation in Epo-GFP transgenic reporter assays. Flow cytometry analyses revealed that the GFP fraction constitutes approximately 0.2% of the whole kidney cells and 63% of GFP-positive cells co-express CD73 (a marker for cortical fibroblasts and Epo-expressing cells in the kidney). Quantitative RT-PCR analyses confirmed that Epo expression was increased by approximately 100-fold in the purified population of REPs compared with that of the unsorted cells or CD73-positive fraction. Gene expression analyses showed enrichment of Hif2α and Hif3α mRNA in the purified population of REPs. The genetic approach described here provides a means to isolate a pure population of REPs, allowing the analysis of gene expression of a defined population of cells essential for Epo production in the kidney. This has provided evidence that positive regulation by HIF2α and negative regulation by HIF3α might be necessary for correct renal Epo induction. (282 words

Minimally Invasive Spinal Stabilization with Denosumab before Total Spondylectomy for a Collapsing Lower Lumbar Spinal Giant Cell Tumor

A 21-year-old man consulted our hospital for treatment of a spinal giant cell tumor (GCT) of Enneking stage III. Lower lumbar-spine tumors and severe spinal canal stenosis are associated with high risk for surgical mor-bidity. Stability was temporarily secured with a percutaneous pedicle screw fixation in combination with deno-sumab, which shrank the tumor. Total en bloc spondylectomy was then performed 6 months after initiation of denosumab, and the patient was followed for 3 years. There was no local recurrence, and bony fusion was obtained. Minimally invasive surgery and denosumab allowed safer and easier treatment of a collapsing lower lumbar extra-compartmental GCT

Dynamically Integrated Transport Approach for High-Energy Nuclear Collisions at High Baryon Density

To explore the structure of the QCD phase diagram in high baryon density domain, several high-energy nuclear collision experiments in a wide range of beam energies are currently performed or planned using many accelerator facilities. In these experiments search for a first-order phase transition and the QCD critical point is one of the most important topics. To find the signature of the phase transition, experimental data should be compared to appropriate dynamical models which quantitatively describe the process of the collisions. In this study we develop a new dynamical model on the basis of the non-equilibrium hadronic transport model JAM and 3+1D hydrodynamics. We show that the new model reproduce well the experimental beam-energy dependence of hadron yields and particle ratio by the partial thermalization of the system in our core-corona approach.Comment: 4 pages, 3 figures; contribution to the proceedings of the 8th International Conference on Quarks and Nuclear Physics (QNP2018), Tsukuba, November 13-17, 201

A Common Proper Motion Stellar Companion to HAT-P-7

We report that HAT-P-7 has a common proper motion stellar companion. The companion is located at approx. 3.9 arcsec to the east and estimated as an M5.5V dwarf based on its colors. We also confirm the presence of the third companion, which was first reported by Winn et al. (2009), based on long-term radial velocity measurements. We revisit the migration mechanism of HAT-P-7b given the presence of those companions, and propose sequential Kozai migration as a likely scenario in this system. This scenario may explain the reason for an outlier in the discussion of the spin-orbit alignment timescale for HAT-P-7b by Albrecht et al. (2012)

Traumatic Globe Luxation with Complete Optic Nerve Transection Caused by Heavy Object Compression

Traumatic eyeball luxation is a rare clinical condition with a dramatic presentation. Here, we describe a unique case of traumatic globe luxation and complete optic nerve transection caused by heavy object compression. A 45-year-old male automobile mechanic was injured when a truck slipped from its supports, crushing his head and face. On arrival, his right eyeball was obviously displaced anteriorly and he had no light perception. Computed tomography revealed complex frontal bone and facial fractures with underlying brain contusion in addition to complete transection of the right optic nerve. The patient was successfully treated using a multidisciplinary approach