Bordetella pertussis population dynamics and phylogeny in Japan after adoption of acellular pertussis vaccines

Bordetella pertussis, the causative agent of whooping cough, has experienced a resurgence in the past 15 years, despite the existence of both whole-cell and acellular vaccines. Here, we performed whole genome sequencing analysis of 149 clinical strains, provided by the National Institute of Infectious Diseases (NIID), Japan, isolated in 1982-2014, after Japan became the first country to adopt acellular vaccines against B. pertussis. Additionally, we sequenced 39 strains provided by the Konan Kosei Hospital in Aichi prefecture, Japan, isolated in 2008-2013. The genome sequences afforded insight into B. pertussis genome variability and population dynamics in Japan, and revealed that the B. pertussis population in Japan was characterized by two major clades that divided more than 40 years ago. The pertactin gene was disrupted in about 20 % of the 149 NIID isolates, by either a deletion within the signal sequence (ΔSS) or the insertion of IS element IS481 (prn :: IS481). Phylogeny suggests that the parent clones for these isolates originated in Japan. Divergence dating traced the first generation of the pertactin-deficient mutants in Japan to around 1990, and indicated that strains containing the alternative pertactin allele prn2 may have appeared in Japan around 1974. Molecular clock data suggested that observed fluctuations in B. pertussis population size may have coincided with changes in vaccine usage in the country. The continuing failure to eradicate the disease warrants an exploration of novel vaccine compositions

Early Results of a Wildfire Monitoring Microsatellite UNIFORM-1

UNIFORM (UNiversity International FORmation Mission) is a capacity building program in microsatellite field including satellites, ground stations, and data platform. The program, sponsored by the Ministry of Education, Culture, Sports and Technology (MEXT) of Japan, aims to increase the number of players in the small satellite community through education of both domestic and international young engineers, by providing them with an opportunity to study, build, and operate microsatellites. The first satellite of the program, UNIFORM-1 was launched on May 24th 2014. UNIFORM-1 is a 50-kg earth observation satellite whose mission is wildfiremonitoring using a microbolometer. Since then it has been in operation, successfully capturing several events on the ground including wildfires and volcanic activities. This paper presents in-orbit results of UNIFORM-1 mission, critical bus subsystems including EPS and AOCS, and lessons learned from its operations

The safety for drinking of hot spring hydrogen water

The safety for drinking of hot spring hydrogen water was examined for 27 healthy women. The research method was the crossover comparison examination of hot spring hydrogen water and tap water for four weeks. The blood cell count, liver function, kidney function, plasma lipids, and electrolytes were measured before and after drinking. Mean corpuscular volume and mean corpuscular hemoglobin concentration significantly decreased after drinking of hot spring hydrogen water and tap water. The plasma total protein, blood urea nitrogen, and total cholesterol significantly decreased after drinking the hot spring hydrogen water. Other data showed no significant changes. These results suggested that the drinking of hot spring hydrogen water was almost safe

PAX5 alterations in an infant case of KMT2A-rearranged leukemia with lineage switch

Lineage switch is a rare event at leukemic relapse. While mostly known to occur in KMT2A-rearranged infant leukemia, the underlying mechanism is yet to be depicted. This case report describes a female infant who achieved remission of KMT2A-MLLT3-rearranged acute monocytic leukemia, but 6 months thereafter, relapsed as KMT2A-MLLT3-rearranged acute lymphocytic leukemia. Whole exome sequencing of the bone marrow obtained pre-post lineage switch revealed two somatic mutations of PAX5 in the relapse sample. These two PAX5 alterations were suggested to be loss of function, thus to have played the driver role in the lineage switch from acute monocytic leukemia to acute lymphocytic leukemia