Teaching old NCATs new tricks: using non-canonical amino acid tagging to study neuronal plasticity

The non-canonical amino acid labeling techniques BONCAT (bioorthogonal non-canonical amino acid tagging) and FUNCAT (fluorescent non-canonical amino acid tagging) enable the specific identification and visualization of newly synthesized proteins. Recently, these techniques have been applied to neuronal systems to elucidate protein synthesis dynamics during plasticity, identify stimulation-induced proteomes and subproteomes and to investigate local protein synthesis in specific subcellular compartments. The next generation of tools and applications, reviewed here, includes the development of new tags, the quantitative identification of newly synthesized proteins, the application of NCAT to whole animals, and the ability to genetically restrict NCAT labeling. These techniques will enable not only improved detection but also allow new scientific questions to be tackled

Spitzer Observations of Cold Dust Galaxies

We combine new Spitzer Space Telescope observations in the mid- and far-infrared with SCUBA 850 micron observations to improve the measurement of dust temperatures, masses and luminosities for 11 galaxies of the SCUBA Local Universe Galaxy Survey (SLUGS). By fitting dust models we measure typical dust masses of 10E7.9 M_sol and dust luminosities of ~ 10E10 L_sol, for galaxies with modest star formation rates. The data presented in this paper combined with previous observations show that cold dust is present in all types of spiral galaxies and is a major contributor to their total luminosity. Because of the lower dust temperature of the SCUBA sources measured in this paper, they have flatter Far-IR nu F_nu(160um)/nu F_nu(850um) slopes than the larger Spitzer Nearby Galaxies Survey (SINGS), the sample that provides the best measurements of the dust properties of galaxies in the nearby universe. The new data presented here added to SINGS extend the parameter space that is well covered by local galaxies, providing a comprehensive set of templates that can be used to interpret the observations of nearby and distant galaxies.Comment: Accepted by A.J. 16 pages, 10 figures, 7 tables. High resolution version at http://mips.as.arizona.edu/~cnaw/slugs_hires.pd

Probing the origin of extragalactic magnetic fields with Fast Radio Bursts

The joint analysis of the Dispersion and Faraday Rotation Measure from distant, polarised Fast Radio Bursts may be used to put constraints on the origin and distribution of extragalactic magnetic fields on cosmological scales. While the combination of Dispersion and Faraday Rotation Measure can in principle give the average magnetic fields along the line-of-sight,in practice this method must be used with care because it strongly depends on the assumed magnetisation model on large cosmological scales. Our simulations show that the observation of Rotation Measures with greater than or equal to 1 − 10 rad/m2 in ∼ 10^2 − 10^3 Fast Radio Bursts will likely be able to discriminate between extreme scenarios for the origin of cosmic magnetic fields, independent of the exact distribution of sources with redshift. This represent a strong case for incoming (e.g. ALERT, CHIME) and future (e.g. with the Square Kilometer Array) radio polarisation surveys of the sky

Hunting for planets in the HL Tau disk

Recent ALMA images of HL Tau show gaps in the dusty disk that may be caused by planetary bodies. Given the young age of this system, if confirmed, this finding would imply very short timescales for planet formation, probably in a gravitationally unstable disk. To test this scenario, we searched for young planets by means of direct imaging in the L'-band using the Large Binocular Telescope Interferometer mid-infrared camera. At the location of two prominent dips in the dust distribution at ~70AU (~0.5") from the central star we reach a contrast level of ~7.5mag. We did not detect any point source at the location of the rings. Using evolutionary models we derive upper limits of ~10-15MJup at <=0.5-1Ma for the possible planets. With these sensitivity limits we should have been able to detect companions sufficiently massive to open full gaps in the disk. The structures detected at mm-wavelengths could be gaps in the distributions of large grains on the disk midplane, caused by planets not massive enough to fully open gaps. Future ALMA observations of the molecular gas density profile and kinematics as well as higher contrast infrared observations may be able to provide a definitive answer.Comment: Accepted for publication on ApJ Letter

Associations between anxiety, body mass index, and sex hormones in women

Background: Several studies have shown a positive association between anxiety and obesity, particularly in women. We aimed to study whether sex hormone alterations related to obesity might play a role in this association. Patients and methods: Data for this study were obtained from a population-based cohort study (the LIFE-Adult-Study). A total of 3,124 adult women (970 premenopausal and 2,154 postmenopausal) were included into the analyses. The anxiety symptomatology was assessed using the GAD-7 questionnaire (cut-off ≥ 10 points). Sex hormones were measured from fasting serum samples. Results: We did not find significant differences in anxiety prevalence in premenopausal obese women compared with normal-weight controls (4.8% vs. 5.5%). Both obesity and anxiety symptomatology were separately associated with the same sex hormone alteration in premenopausal women: higher total testosterone level (0.97 ± 0.50 in obese vs. 0.86 ± 0.49 nmol/L in normal-weight women, p = 0.026 and 1.04 ± 0.59 in women with vs. 0.88 ± 0.49 nmol/L in women without anxiety symptomatology, p = 0.023). However, women with anxiety symptomatology had non-significantly higher estradiol levels than women without anxiety symptomatology (548.0 ± 507.6 vs. 426.2 ± 474.0 pmol/L), whereas obesity was associated with lower estradiol levels compared with those in normal-weight group (332.7 ± 386.5 vs. 470.8 ± 616.0 pmol/L). Women with anxiety symptomatology had also significantly higher testosterone and estradiol composition (p = 0.006). No associations of sex hormone levels and BMI with anxiety symptomatology in postmenopausal women were found. Conclusions: Although both obesity and anxiety symptomatology were separately associated with higher testosterone level, there was an opposite impact of anxiety and obesity on estradiol levels in premenopausal women. We did not find an evidence that the sex hormone alterations related to obesity are playing a significant role in anxiety symptomatology in premenopausal women. This could be the explanation why we did not find an association between obesity and anxiety. In postmenopausal women, other mechanisms seem to work than in the premenopausal group

A disrupted circumstellar torus inside eta Carinae's Homunculus Nebula

We present thermal infrared images of the bipolar nebula surrounding eta Carinae at six wavelengths from 4.8 to 24.5 microns. These were obtained with the MIRAC3 camera system at the Magellan Observatory. Our images reveal new intricate structure in the bright core of the nebula, allowing us to re-evaluate interpretations of morphology seen in images with lower resolution. Complex structures in the core might not arise from a pair of overlapping rings or a cool (110 K) and very massive dust torus, as has been suggested recently. Instead, it seems more likely that the arcs and compact knots comprise a warm (350 K) disrupted torus at the intersection of the larger polar lobes. Some of the arcs appear to break out of the inner core region, and may be associated with equatorial features seen in optical images. The torus could have been disrupted by a post-eruption stellar wind, or by ejecta from the Great Eruption itself if the torus existed before that event. Kinematic data are required to rule out either possibility.Comment: 8 pages, 3 figures (Fig. 1 in color); to appear in ApJ Letter