Precipitation forecast input to hydrological modelling

Tables showing patient details according to the genetic background. (DOCX 184 kb

Flow chart of study design.

<p>For biomarker discovery in total 125 seminal plasma samples were used from 70 patients with PCa, 21 patients with benign prostate hyperplasia (BPH), 25 patients with chronic prostatitis (CP) and 9 healthy control (HC). This pool of available samples was used in varying composition in three study arms. In study A “Diagnostic Markers” 50/125 patients with and without prostate cancer (22 PCa, 14 CP; 9 BPH and 5 HC) were used for biomarker discovery and the remaining 75/125 patients (48 PCa, 12 BPH, 11 CP, and 4HC) were used for diagnostic performance tests. In Study B “Advanced Disease Markers” available PCa samples (n = 70) were stratified according to Gleason score. For biomarker discovery patients with Gleason score <7 (n = 21) and Gleason score >7 (n = 16) were compared. The remaining 33/70 patients with Gleason score 7 (28 indolent disease </p

Biomarker signatures.

<p>Normalized molecular weight (700–25.000 Da) in logarithmic scale is plotted against normalized migration time (15–45 min). The mean signal intensity of the polypeptide peak is given in 3D-depiction. Averaged data sets of the training set are shown.</p

Polypeptides constituting the biomarker signatures 21PP, 5PP, and 11PP, respectively.

<p>ID: polypeptide identifier annotated by the SQL database (ID).</p>+<p>: upregulated biomarkers: mean(case)/mean(control);</p><p>downregulated biomarkers: -mean(control)/mean(case).</p>#<p>: Biomarker of 21PP and 11PP.</p

Patient descriptive statistics.

<p>CP  =  chronic prostatitis; HC  =  healthy control; BPH  =  benign prostata hyperplasia; PCa  =  prostate carcinoma; n.a.  =  not applicable or not available; §  =  sign. vs. BPH; $  =  sign. vs. PCa (two-tailed Kruskal-Wallis parameter free ANOVA with Dunn’s Multiple Comparison Test); D’Amico <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0067514#pone.0067514-DAmico1" target="_blank">[28]</a> adopted by the AUA  =  American Urology Association <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0067514#pone.0067514-Hanno1" target="_blank">[47]</a>; NCCN  =  National Comprehensive Cancer Network <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0067514#pone.0067514-Mohler1" target="_blank">[29]</a>; EAU  =  European Association of Urology <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0067514#pone.0067514-Heidenreich1" target="_blank">[31]</a>; RTOG  =  Radiation Therapy Oncology Group <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0067514#pone.0067514-Roach1" target="_blank">[30]</a>; CAPRA  =  Cancer of the Prostate Risk Assessment Score <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0067514#pone.0067514-Cooperberg2" target="_blank">[48]</a>.</p

Biomarker performance validation.

<p>(<b>A</b>).Box and whisker plots of obtained 11PP results in the test cohort of PCa patients with GS 7 stratified according to TNM, (<b>B</b>) EAU, and (<b>C</b>) NCCN classification systems. Black squares indicate medians and whiskers 1.5-times the interquartile ranges. Rank correlation coefficients rho, the respective 95% CI and P-values are given above. (<b>D</b>) ROC curve (black lines) for 11PP classification of the independent validation cohort of PCa patients with GS 7 with either indolent (N = 28) or advanced (N = 5) disease according to EAU classification as reference standard. 95% confidence intervals are plotted as dashed lines. Diagonal line represents guessing probability with an area under the curve of 0.5. 95% confidence intervals (CI) are displayed as dashed lines.</p

Biomarker sequence data.

<p>ID: polypeptide identifier annotated by the SQL database (ID); Theo. Mass: theoretical mass of the peptide sequence; ▵M: Mass difference between experimental and theoretical mass normalized to theoretical mass in parts per million [ppm]. m: oxidized Methionine.</p

Assessment of biomarker stability and reproducibility.

<p>(<b>A</b>) An average of 1887±202 polypeptides was detected in each of the 14 measurements stored for different times at RT. The mean is marked with a bold line; standard deviation is highlighted in grey. (<b>B</b>) Beyond this qualitative assessment biomarker signatures were applied to the 14 stability replicates to obtain quantitative data of time-dependent stability of seminal plasma. For 21PP ranked correlation analysis revealed a significant decrease of SVM scores over time with Spearman’s rho of –0.576 (95% CI –0.854 to –0.07, P = 0.0379). Regression analysis unveiled a decrease rate of –0.05 a.u. (<2%) per hour. 5PP and 11PP displayed no significant time dependency. (<b>C</b>) Analytical precision of the established SVM classifiers was assessed by applying it to 15 CE-MS data sets obtained from independent replicates of a sample of a 57 years old patient with significant BPH. Mean classification scores were 0.619±0.07, 2.290±0.81, and -1.239±0.18 for 21PP, 5PP, and 11PP respectively. Coefficients of variations were calculated by dividing standard deviations by the observed overall range of SVM scores [highlighted in grey, 21PP from –1.50 to +1.50 (3.0 a.u.), 5PP from 4.50 to +3.0 (7.5 a.u.), and 11PP from –1.50 to +1.50 (3.0 a.u.)]. Coefficients of variations were 2.2%, 10.8%, and 6.1%, respectively. Classification cut offs are represented by horizontal lines. The boxes depict means and standard deviation as whiskers.</p

Additional file 3: Figure S3. of Inhibition of fatty acid desaturation is detrimental to cancer cell survival in metabolically compromised environments

Spectrum of free fatty acids of DU145 cells after SCD inhibition. DU145 cells were treated with SCD inhibitors (100nM) or solvent (DMSO) for 48hrs in medium with low serum or supplemented with BSA or BSA-coupled oleic acid (BSA-oleate). (a) Amounts of different FFA species. (b) Amounts of selected saturated and mono-unsaturated FFAs. (c) Relative contribution of FFA species to total FFA pool. (d) Relative contribution of selected saturated and mono-unsaturated FFA species to total FFA pool. Data represent mean ±SEM of three independent biological replicates. Statistical comparisons were performed using Student t-test (*p≤0.05). (PDF 648 kb

Project of Politeia as a base for impletion cross theme on education of a democracy citizen at the base level of primary school

I'm focused on the education of democratic citizen in the base level of Primary School in my diploma work "The project of Politeia as a base for impletion cross theme on education of a democracy citizen at the base level of primary school". I'm giving an information connected with education of citizen culture with general educational programme at primary schools and finally with the project Politeia which was the fundamental idea and impulse for this work. One part of this work is the analysis of the reform in the sphere of citizen culture education, aims and methods. The empirical part in focused on a creation of materials to be used for the realisation of the Cross Theme Education of a Democratic Citizen that gives full use at the base level of a primary school. The mentioned project brings working sheets above all that are accompanied by additional possible activities - for example malingering game. The empirical part was verified in practice at the second and third stage of the Primary School in Prague and it was appraised by teachers with different duration of their teaching career