Neurobiological effects of probiotic-supplemented diets in acutely stressed male Long-Evans rats: Evidence of enhanced resilience

Introduction ~Considering that the human intestine is home to almost 100 trillion microorganisms including bacteria (Cryan, 2011), recent research has focused on the role of the microbiome in neurobiological functions such as stress, anxiety and coping responses. ~Focusing on animal models, previous findings indicate that modifications of the gut microbiota via antibiotics and certain probiotics alter the anxiety response via the vagus nerve & immune system mediation (MacQueen et al., 2017). ~Recently, the concept of Psychobiotics has been introduced to refer to the use of microbiota to positively influence mental health outcomes (Foster et al., 2017)

The Raccoon Project: Exploring Optimal Neural Functions in a Novel Model Species

This study explored the different determinants of optimal neural functions in raccoons as a novel scientific model, this investigation included the study of behavioral and cognitive flexibility, hormonal changes, and brain markers such as neural counts

The Raccoon Project: Exploring Optimal Neural Functions in a Novel Model Species

This study explored the different determinants of optimal neural functions in raccoons as a novel scientific model; specifically, this investigation this included the study of behavioral and cognitive flexibility, hormonal changes, and brain markers such as neural counts. Behavioral testing was conducted at the USDA National Wildlife Research Center – Predator Research Facility in Ft. Collins, CO in collaboration with Dr. Sarah Benson-Amram’s research team at the University of Wyoming. The behavioral assessments allowed for the classification of the animals into a solvers group or a non-solvers group. Fecal samples were used to determine hormonal markers of resilience such as DHEA, Corticosterone (CORT) and the DHEA/CORT ratio. Brain cell counts using DAPI and NeuN staining were determined through the use of the isotropic fractionation method. In general, the findings suggested some markers of emotional resilience in Raccoons through the study of their hormonal changes. In addition, we were able to learn more about the brain structure and cell counts of these novel model species. In sum, this study explored the connections between cognitive flexibility and urban adaptation in terms of cognitive flexibility and immunohistochemical indicators in the brains of raccoons

Cytoarchitectural characteristics associated with cognitive flexibility in raccoons

With rates of psychiatric illnesses such as depression continuing to rise, additional preclinical models are needed to facilitate translational neuroscience research. In the current study, the raccoon (Procyon lotor) was investigated due to its similarities with primate brains, including comparable proportional neuronal densities, cortical magnification of the forepaw area, and cortical gyrification. Specifically, we report on the cytoarchitectural characteristics of raccoons profiled as high, intermediate, or low solvers in a multiaccess problem-solving task. Isotropic fractionation indicated that high-solvers had significantly more cells in the hippocampus (HC) than the other solving groups; further, a nonsignificant trend suggested that this increase in cell profile density was due to increased nonneuronal (e.g., glial) cells. Group differences were not observed in the cellular density of the somatosensory cortex. Thionin-based staining confirmed the presence of von Economo neurons (VENs) in the frontoinsular cortex, although no impact of solving ability on VEN cell profile density levels was observed. Elongated fusiform cells were quantified in the HC dentate gyrus where high-solvers were observed to have higher levels of this cell type than the other solving groups. In sum, the current findings suggest that varying cytoarchitectural phenotypes contribute to cognitive flexibility. Additional research is necessary to determine the translational value of cytoarchitectural distribution patterns on adaptive behavioral outcomes associated with cognitive performance and mental health

Cytoarchitectural Characteristics Associated with Cognitive Flexibility in Raccoons

With rates of psychiatric illnesses such as depression continuing to rise, additional preclinical models are needed to facilitate translational neuroscience research. In the current study, the raccoon (Procyon lotor) was investigated due to its similarities with primate brains, including comparable proportional neuronal densities, cortical magnification of the forepaw area, and cortical gyrification. Specifically, we report on the cytoarchitectural characteristics of raccoons profiled as high, intermediate, or low solvers in a multiaccess problem-solving task. Isotropic fractionation indicated that high-solvers had significantly more cells in the hippocampus (HC) than the other solving groups; further, a nonsignificant trend suggested that this increase in cell profile density was due to increased nonneuronal (e.g., glial) cells. Group differences were not observed in the cellular density of the somatosensory cortex. Thionin-based staining confirmed the presence of von Economo neurons (VENs) in the frontoinsular cortex, although no impact of solving ability on VEN cell profile density levels was observed. Elongated fusiform cells were quantified in the HC dentate gyrus where high-solvers were observed to have higher levels of this cell type than the other solving groups. In sum, the current findings suggest that varying cytoarchitectural phenotypes contribute to cognitive flexibility. Additional research is necessary to determine the translational value of cytoarchitectural distribution patterns on adaptive behavioral outcomes associated with cognitive performance and mental health