Congenital Adrenal Hyperplasia: The Clinical Problem, and the Application of a New Method of Chromatography of the 17 Ketogenic Steroids to the Diagnosis and the Control of Treatment of Congenital Adrenal Hyperplasia

Abstract Not Provided

Impact of Food Assistance Programs on Food Insecurity, Diet Quality and Obesity

Obesity remains a public health issue in the United States because it contributes to chronic diseases. The Supplemental Nutrition Assistance Program (SNAP) was designed to increase food security, alleviate hunger, and increase access to a healthful diet; however, it may have the opposite effect and contribute to obesity. The purpose of this study was to examine to what extent participation in SNAP impacts food insecurity, diet quality, and obesity in U.S. adults. The social-ecological model guided the study which was conducted using a quantitative a cross-sectional research design and secondary analyses of the 2013-2014 National Health and Nutrition Examination Survey (NHANES). The sample consisted of all adults 25 years and older included in the NHANES. Logistic regression analysis results indicated marginal food security was associated with obesity among SNAP (OR = 1.28) and NON-SNAP (OR = 1.54). Full food security was associated with obesity (OR = 1.65) only among NON-SNAP. Among both groups, the greater the diet quality reported the greater the odds of obesity. Poverty mediated the association between marginal food security and obesity only among NON-SNAP participants. Adjusting for socio-economic factors SNAP modified the effect between food security and obesity (OR = 1.30) and diet quality was associated with obesity (OR = 1.72). The results of this study may be uses as support for policies and programs to improve the nutritional impact of SNAP and targeted interventions to address food security in low-income adults

Covering Kids & Families: A Continuing Program for Increasing Insurance Coverage Among Low-Income Families

Explores the impact of the program's coalition-based approaches to improving Children's Health Insurance and Medicaid programs to increase enrollment on policy and procedures and assesses the outcomes of policy change on coverage of children and parents

Recent study brings good news about the soybean aphid

Increased activity of bean leaf beetles and soybean aphids in Iowa soybean fields has challenged many of us over the last five years. Not just because of the sap feeding and leaf defoliation that can cause significant yield loss but also because we are dealing with another yield robber that we often can\u27t see. Iowa soybean fields can be infected with bean pod mottle and soybean {m}osaic viruses that are transmitted by bean leaf beetles and soybean aphids, respectively. The challenge that we are dealing with is, first of all, we can\u27t always see that we have a virus in soybean, and yield loss caused by viruses can occur even when symptoms are not apparent

IMPACT: The Journal of the Center for Interdisciplinary Teaching and Learning. Volume 9, Issue 1, Winter 2020

Explicitly established to foreground interdisciplinary teaching and learning, Impact also welcomes evidence and discussion of experiential learning. Often the two – interdisciplinary teaching and experiential learning – co-exist. Yet even when they do not, both practices model how to think in myriad ways and to notice how knowledge is constructed. As our winter 2019 issue makes clear, interdisciplinary teaching and learning and experiential learning often begin with questions. Why does it matter that students grapple directly with archival material? What happens when undergraduates practice psychology by training dogs? Do students understand financial literacy? This issue also asks questions about students’ reading habits and faculty expectations of them as readers

The potency of the broad spectrum bacteriocin, bactofencin A, against staphylococci is highly dependent on primary structure, N-terminal charge and disulphide formation

Bactofencin A is a novel class IId bacteriocin, produced by the intestinal isolate Lactobacillus salivarius DPC6502, which has potent activity against medically significant pathogens including Staphylococcus aureus. This bacteriocin is unusual in that it has a highly cationic N terminus and a single disulfide bond between Cys7 and Cys22, resulting in a large C terminal loop. In this study, a library of synthetic bactofencin A variants were screened against the mastitis isolate, S. aureus DPC5246, to identify key residues responsible for activity. It was apparent that substituting either cysteine of the disulfide bond with either serine or alanine significantly reduced the activity of the bacteriocin, confirming the importance of the C terminal loop. Substituting N terminal amino acids with alanine had no effect on activity, whereas sequential removal of the N terminal positively charged residues resulted in an increasingly inactive peptide. A complete (synthetic) alanine scanning analysis revealed that the residues between Val9 and Gly17 were most affected by substitution suggesting that this area has a major influence on the potency of the bacteriocin. Substituting residues in the loop region between Cys7 and Cys22 for D-amino acid equivalents had a more detrimental effect on activity than L-alanine substitutions. Specifically Y10A, N11A, P15A and T16A are active at 4, 16, 1 and 16 μM respectively while their D equivalents were inactive at 1000 μM, the highest concentration tested. Ultimately, this study identifies the critical features in the primary structure of the bacteriocin which gives it such potent activity against pathogenic staphylococci

Informal Science Experiences among Urban and Rural Youth: Exploring Differences at the Intersections of Socioeconomic Status, Gender and Ethnicity

The current study explores patterns of informal science experiences among youth in urban and rural middle schools by gender and socioeconomic status. Data come from surveys in two Midwestern middle schools, one in a mid-sized city, and the other in a rural-remote town. We asked about participation in informal science activities (e.g. visiting zoos or museums, or watching shows about science) and if youth had participated in science-focused clubs in the last 12 months (e.g. after-school science clubs, 4-H, scouts). Rural youth reported lower rates of participation in after-school science clubs and a greater desire to participate in after-school science programming than urban youth. Latino/a youth tend to have fewer informal science experiences than non- Latino/a youth, particularly in urban areas. There were few differences in informal science experiences between boys and girls, but in urban areas, girls report more science experiences than boys. Reported science experiences are overall higher in urban areas, yet youth with fewer resources (i.e. books in the home) have fewer informal science experiences overall. This study sheds new light on how socioeconomic status, gender, ethnicity, and geography interact with one another to shape youth science exposure and interest

MMA, A Computer Code for Multi-Model Analysis

This report documents the Multi-Model Analysis (MMA) computer code. MMA can be used to evaluate results from alternative models of a single system using the same set of observations for all models. As long as the observations, the observation weighting, and system being represented are the same, the models can differ in nearly any way imaginable. For example, they may include different processes, different simulation software, different temporal definitions (for example, steady-state and transient models could be considered), and so on. The multiple models need to be calibrated by nonlinear regression. Calibration of the individual models needs to be completed before application of MMA. MMA can be used to rank models and calculate posterior model probabilities. These can be used to (1) determine the relative importance of the characteristics embodied in the alternative models, (2) calculate model-averaged parameter estimates and predictions, and (3) quantify the uncertainty of parameter estimates and predictions in a way that integrates the variations represented by the alternative models. There is a lack of consensus on what model analysis methods are best, so MMA provides four default methods. Two are based on Kullback-Leibler information, and use the AIC (Akaike Information Criterion) or AICc (second-order-bias-corrected AIC) model discrimination criteria. The other two default methods are the BIC (Bayesian Information Criterion) and the KIC (Kashyap Information Criterion) model discrimination criteria. Use of the KIC criterion is equivalent to using the maximum-likelihood Bayesian model averaging (MLBMA) method. AIC, AICc, and BIC can be derived from Frequentist or Bayesian arguments. The default methods based on Kullback-Leibler information have a number of theoretical advantages, including that they tend to favor more complicated models as more data become available than do the other methods, which makes sense in many situations

Using a College Curriculum to Integrate Informal Science Learning Opportunities for University and Middle School Students

We used community partnerships to develop an integrated science-learning program focused on two groups of learners – university and middle school (MS) students – to increase students’ interest and confidence in science as well as motivation to pursue science. Key program elements include a university course for undergraduate and graduate students, university student-led weekly afterschool clubs held at local middle schools (mostly Title I), and a capstone museum science festival led by university and MS students. Across nine course offerings, 78 university students conducted 25 clubs at seven middle schools and engaged at least 240 MS students. The capstone science festival engaged ~1,200 public participants across six events. We evaluated the program in two phases. Quantitative and qualitative assessments show that university students enjoyed the course and increased their ability to describe complex scientific phenomenon to youth. Middle school students reported significant increases in science interest, science understanding, and understanding scientists (1st evaluation phase); and increased interest in a career in science and in their perception of others seeing them as a scientist (2nd phase). Consistent with prior research, overall we found an increase in interest and understanding of science, science identity, and interest in future science careers for MS students

Relatedness between the two-component lantibiotics lacticin 3147 and staphylococcin C55 based on structure, genetics and biological activity

BACKGROUND: Two component lantibiotics, such as the plasmid-encoded lacticin 3147 produced by Lactococcus lactis DPC3147 and staphylococcin C55 produced by Staphylococcus aureus C55, represent an emerging subgroup of bacteriocins. These two bacteriocins are particularly closely related, exhibiting 86% (LtnA1 and C55α) and 55% (LtnA2 and C55β) identity in their component peptides. The aim of this study was to investigate, for the first time for any two component bacteriocins, the significance of the relatedness between these two systems. RESULTS: So close is this relatedness that the hybrid peptide pairs LtnA1:C55β and C55α:LtnA2 were found to have activities in the single nanomolar range, comparing well with the native pairings. To determine whether this flexibility extended to the associated post-translational modification/processing machinery, the staphylococcin C55 structural genes were directly substituted for their lacticin 3147 counterparts in the ltn operon on the large conjugative lactococcal plasmid pMRC01. It was established that the lacticin LtnA1 post-translational and processing machinery could produce functionally active C55α, but not C55β. In order to investigate in closer detail the significance of the differences between LtnA1 and C55α, three residues in LtnA1 were replaced with the equivalent residues in C55α. Surprisingly, one such mutant LtnA1-Leu21Ala was not produced. This may be significant given the positioning of this residue in a putative lipid II binding loop. CONCLUSION: It is apparent, despite sharing striking similarities in terms of structure and activity, that these two complex bacteriocins display some highly dedicated features particular to either system