Systemic approaches to improvement in sugarcane production and supply : Umfolozi and Felixton Mill areas.

Doctor of Philosophy in Business Administration. University of KwaZulu-Natal, Westville 2013.Within the South African sugar industry, several possibilities for performance improvement exist. Present inefficiencies arise largely from the complexity of integrated sugarcane production and supply systems. Research has mainly concentrated on technical, hard aspects, such as mill and transport efficiency and sugarcane quality in an attempt to optimise these systems by optimising their parts. Soft issues, like communication, trust, and values have been neglected. This study considers sugarcane production and supply systems more holistically and places a particular focus on soft and leadership issues. Two systems methodologies, Soft Systems Methodology (SSM) and the Viable System Model (VSM) were applied to investigate the complexity of two large sugarcane production and supply systems in KwaZulu-Natal, South Africa in the Felixton and Umfolozi milling areas. These methodologies were combined with a qualitative approach which facilitated a thorough exploration of crucial soft and leadership issues. The outcome of the empirical work showed that a core issue challenging both milling areas and the entire sugar industry is the presence of fragmentation. Factors that contribute to fragmentation and suggestions for its handling are presented. SSM and VSM fostered an in-depth understanding of the studied system, yet their ability to suggest improvements was not confirmed. Since it is argued that this was largely impeded by the conditions of the study, the thesis overall supports the suitability of both methodologies in the sugar industry context and encourages their further use. The thesis emphasises the necessity to adopt a holistic approach and pay attention to soft issues when dealing with sugarcane production and supply systems. By implication, systemic approaches in general seem significant in this context. Neither SSM nor VSM were previously utilised to investigate a sugarcane production and supply system, hence this thesis makes a meaningful contribution to the existing body of SSM and VSM knowledge. It highlights the strengths and shortfalls of these systems methodologies in the applied context and presents derived methodological lessons. These lessons broaden the knowledge of employing SSM and VSM and support their application in practice

Inhibition of DNA polymerase reactions by pyrimidine nucleotide analogues lacking the 2-keto group

To investigate the influence of the pyrimidine 2-keto group on selection of nucleotides for incorporation into DNA by polymerases, we have prepared two C nucleoside triphosphates that are analogues of dCTP and dTTP, namely 2-amino-5-(2′-deoxy-β-d-ribofuranosyl) pyridine-5′-triphosphate (d*CTP) and 5-(2′-deoxy-β-d-ribofuranosyl)-3-methyl-2-pyridone-5′-triphosphate (d*TTP) respectively. Both proved strongly inhibitory to PCR catalysed by Taq polymerase; d*TTP rather more so than d*CTP. In primer extension experiments conducted with either Taq polymerase or the Klenow fragment of Escherichia coli DNA polymerase I, both nucleotides failed to substitute for their natural pyrimidine counterparts. Neither derivative was incorporated as a chain terminator. Their capacity to inhibit DNA polymerase activity may well result from incompatibility with the correctly folded form of the polymerase enzyme needed to stabilize the transition state and catalyse phosphodiester bond formatio

Applications of Micro-Raman Spectroscopy in Cultural Heritage – Examples from the Laboratory for Conservation Research of the Collections Centre of the Swiss National Museums

The paper reports on three applications of micro-Raman spectroscopy which were carried out in the Laboratory for Conservation Research of the Collections Centre of the Swiss National Museums. The first application addresses archaeological questions about three early mediaeval fibulae which were decorated with gemstones. The determination of different corrosion products on pigeon cameras by micro-Raman spectroscopy is described in the second part of this article. The last case study discusses an example from the field of preventive conservation where changes on metal coupons from the so-called Oddy test were analysed